Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts

Abstract With the increasing demand for higher gene carrier performance, a multifunctional vector could immensely simplify gene delivery for disease treatment; nevertheless, the current non- viral vectors lack self-tracking ability. Here, a type of novel, dual-functional cationic carbon dots (CDs),...

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Autores principales:	Xia Cao, Jianping Wang, Wenwen Deng, Jingjing Chen, Yan Wang, Jie Zhou, Pan Du, Wenqian Xu, Qiang Wang, Qilong Wang, Qingtong Yu, Myron Spector, Jiangnan Yu, Ximing Xu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a5eade4c344243929a4ad2aafa674839
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spelling	oai:doaj.org-article:a5eade4c344243929a4ad2aafa6748392021-12-02T16:08:01ZPhotoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts10.1038/s41598-018-25330-x2045-2322https://doaj.org/article/a5eade4c344243929a4ad2aafa6748392018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25330-xhttps://doaj.org/toc/2045-2322Abstract With the increasing demand for higher gene carrier performance, a multifunctional vector could immensely simplify gene delivery for disease treatment; nevertheless, the current non- viral vectors lack self-tracking ability. Here, a type of novel, dual-functional cationic carbon dots (CDs), produced through one-step, microwave-assisted pyrolysis of arginine and glucose, have been utilized as both a self-imaging agent and a non-viral gene vector for chondrogenesis from fibroblasts. The cationic CDs could condense the model gene plasmid SOX9 (pSOX9) to form ultra-small (10–30 nm) nanoparticles which possessed several favorable properties, including high solubility, tunable fluorescence, high yield, low cytotoxicity and outstanding biocompatibility. The MTT assay indicated that CDs/pSOX9 nanoparticles had little cytotoxicity against mouse embryonic fibroblasts (MEFs) compared to Lipofectamine2000 and PEI (25 kDa). Importantly, the CDs/pSOX9 nanoparticles with tunable fluorescence not only enabled the intracellular tracking of the nanoparticles, but also could successfully deliver the pSOX9 into MEFs with significantly high efficiency. Furthermore, the CDs/pSOX9 nanoparticles-mediated transfection of MEFs showed obvious chondrogenic differentiation. Altogether, these findings demonstrated that the CDs prepared in this study could serve as a paradigmatic example of the dual-functional reagent for both self-imaging and effective non-viral gene delivery.Xia CaoJianping WangWenwen DengJingjing ChenYan WangJie ZhouPan DuWenqian XuQiang WangQilong WangQingtong YuMyron SpectorJiangnan YuXiming XuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xia Cao Jianping Wang Wenwen Deng Jingjing Chen Yan Wang Jie Zhou Pan Du Wenqian Xu Qiang Wang Qilong Wang Qingtong Yu Myron Spector Jiangnan Yu Ximing Xu Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts
description	Abstract With the increasing demand for higher gene carrier performance, a multifunctional vector could immensely simplify gene delivery for disease treatment; nevertheless, the current non- viral vectors lack self-tracking ability. Here, a type of novel, dual-functional cationic carbon dots (CDs), produced through one-step, microwave-assisted pyrolysis of arginine and glucose, have been utilized as both a self-imaging agent and a non-viral gene vector for chondrogenesis from fibroblasts. The cationic CDs could condense the model gene plasmid SOX9 (pSOX9) to form ultra-small (10–30 nm) nanoparticles which possessed several favorable properties, including high solubility, tunable fluorescence, high yield, low cytotoxicity and outstanding biocompatibility. The MTT assay indicated that CDs/pSOX9 nanoparticles had little cytotoxicity against mouse embryonic fibroblasts (MEFs) compared to Lipofectamine2000 and PEI (25 kDa). Importantly, the CDs/pSOX9 nanoparticles with tunable fluorescence not only enabled the intracellular tracking of the nanoparticles, but also could successfully deliver the pSOX9 into MEFs with significantly high efficiency. Furthermore, the CDs/pSOX9 nanoparticles-mediated transfection of MEFs showed obvious chondrogenic differentiation. Altogether, these findings demonstrated that the CDs prepared in this study could serve as a paradigmatic example of the dual-functional reagent for both self-imaging and effective non-viral gene delivery.
format	article
author	Xia Cao Jianping Wang Wenwen Deng Jingjing Chen Yan Wang Jie Zhou Pan Du Wenqian Xu Qiang Wang Qilong Wang Qingtong Yu Myron Spector Jiangnan Yu Ximing Xu
author_facet	Xia Cao Jianping Wang Wenwen Deng Jingjing Chen Yan Wang Jie Zhou Pan Du Wenqian Xu Qiang Wang Qilong Wang Qingtong Yu Myron Spector Jiangnan Yu Ximing Xu
author_sort	Xia Cao
title	Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts
title_short	Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts
title_full	Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts
title_fullStr	Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts
title_full_unstemmed	Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts
title_sort	photoluminescent cationic carbon dots as efficient non-viral delivery of plasmid sox9 and chondrogenesis of fibroblasts
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a5eade4c344243929a4ad2aafa674839
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Photoluminescent Cationic Carbon Dots as efficient Non-Viral Delivery of Plasmid SOX9 and Chondrogenesis of Fibroblasts

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