Vitrification Ability of Combined and Single Cryoprotective Agents

Cryoprotective agents (CPA) are an important part of many current vitrification methods. The vitrification ability of CPAs influences the probability of the glass transition and water crystallization occurrence. Thermal characteristics and the vitrification ability of two combined CPAs (PVS2 and PVS...

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Autores principales:	Milos Faltus, Alois Bilavcik, Jiri Zamecnik
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	cryopreservation cryoprotectant differential scanning calorimetry glass transition vitrification water crystallization Botany QK1-989
Acceso en línea:	https://doaj.org/article/a5eafbe19bd348c78ae2ed587f07a333
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spelling	oai:doaj.org-article:a5eafbe19bd348c78ae2ed587f07a3332021-11-25T18:46:14ZVitrification Ability of Combined and Single Cryoprotective Agents10.3390/plants101123922223-7747https://doaj.org/article/a5eafbe19bd348c78ae2ed587f07a3332021-11-01T00:00:00Zhttps://www.mdpi.com/2223-7747/10/11/2392https://doaj.org/toc/2223-7747Cryoprotective agents (CPA) are an important part of many current vitrification methods. The vitrification ability of CPAs influences the probability of the glass transition and water crystallization occurrence. Thermal characteristics and the vitrification ability of two combined CPAs (PVS2 and PVS3), common plant vitrification solutions, and four single CPAs (ethylene glycol, DMSO, glycerol, and sucrose), the components of the mentioned PVSs, were evaluated utilizing a differential scanning calorimetry (DSC) during standard cooling/warming rates of 10 °C min<sup>−1</sup>. The effect of solute concentration on their vitrification ability was shown in the CPAs tested. Four typical concentration regions at which the glassy state and/or crystallization occurred were defined. We suggest the solute concentration of 0.7 g g<sup>−1</sup> as the universal vitrification concentration, characterized by an actual Tg of CPA solution and limited water crystallization. Knowledge of the thermal properties of CPAs allows the design of new combined CPAs with the required vitrification ability respecting the cryopreservation method used and the characteristics of the cryopreserved sample.Milos FaltusAlois BilavcikJiri ZamecnikMDPI AGarticlecryopreservationcryoprotectantdifferential scanning calorimetryglass transitionvitrificationwater crystallizationBotanyQK1-989ENPlants, Vol 10, Iss 2392, p 2392 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cryopreservation cryoprotectant differential scanning calorimetry glass transition vitrification water crystallization Botany QK1-989
spellingShingle	cryopreservation cryoprotectant differential scanning calorimetry glass transition vitrification water crystallization Botany QK1-989 Milos Faltus Alois Bilavcik Jiri Zamecnik Vitrification Ability of Combined and Single Cryoprotective Agents
description	Cryoprotective agents (CPA) are an important part of many current vitrification methods. The vitrification ability of CPAs influences the probability of the glass transition and water crystallization occurrence. Thermal characteristics and the vitrification ability of two combined CPAs (PVS2 and PVS3), common plant vitrification solutions, and four single CPAs (ethylene glycol, DMSO, glycerol, and sucrose), the components of the mentioned PVSs, were evaluated utilizing a differential scanning calorimetry (DSC) during standard cooling/warming rates of 10 °C min<sup>−1</sup>. The effect of solute concentration on their vitrification ability was shown in the CPAs tested. Four typical concentration regions at which the glassy state and/or crystallization occurred were defined. We suggest the solute concentration of 0.7 g g<sup>−1</sup> as the universal vitrification concentration, characterized by an actual Tg of CPA solution and limited water crystallization. Knowledge of the thermal properties of CPAs allows the design of new combined CPAs with the required vitrification ability respecting the cryopreservation method used and the characteristics of the cryopreserved sample.
format	article
author	Milos Faltus Alois Bilavcik Jiri Zamecnik
author_facet	Milos Faltus Alois Bilavcik Jiri Zamecnik
author_sort	Milos Faltus
title	Vitrification Ability of Combined and Single Cryoprotective Agents
title_short	Vitrification Ability of Combined and Single Cryoprotective Agents
title_full	Vitrification Ability of Combined and Single Cryoprotective Agents
title_fullStr	Vitrification Ability of Combined and Single Cryoprotective Agents
title_full_unstemmed	Vitrification Ability of Combined and Single Cryoprotective Agents
title_sort	vitrification ability of combined and single cryoprotective agents
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a5eafbe19bd348c78ae2ed587f07a333
work_keys_str_mv	AT milosfaltus vitrificationabilityofcombinedandsinglecryoprotectiveagents AT aloisbilavcik vitrificationabilityofcombinedandsinglecryoprotectiveagents AT jirizamecnik vitrificationabilityofcombinedandsinglecryoprotectiveagents
_version_	1718410732224118784

Vitrification Ability of Combined and Single Cryoprotective Agents

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