Constraint-based modeling of yeast mitochondria reveals the dynamics of protein import and iron-sulfur cluster biogenesis

Summary: Mitochondria are a hallmark of eukaryal cells and play an important role in cellular metabolism. There is a vast amount of knowledge available on mitochondrial metabolism and essential mitochondrial functions, such as protein import and iron-sulfur cluster biosynthesis, including multiple s...

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Autores principales:	Carl Malina, Francesca Di Bartolomeo, Eduard J. Kerkhoven, Jens Nielsen
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cellular physiology Cell biology Integrative aspects of cell biology Systems biology In silico biology Science Q
Acceso en línea:	https://doaj.org/article/a5f29949808a4ea5b5bf307bdf677aaf
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spelling	oai:doaj.org-article:a5f29949808a4ea5b5bf307bdf677aaf2021-11-20T05:09:33ZConstraint-based modeling of yeast mitochondria reveals the dynamics of protein import and iron-sulfur cluster biogenesis2589-004210.1016/j.isci.2021.103294https://doaj.org/article/a5f29949808a4ea5b5bf307bdf677aaf2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221012633https://doaj.org/toc/2589-0042Summary: Mitochondria are a hallmark of eukaryal cells and play an important role in cellular metabolism. There is a vast amount of knowledge available on mitochondrial metabolism and essential mitochondrial functions, such as protein import and iron-sulfur cluster biosynthesis, including multiple studies on the mitochondrial proteome. Therefore, there is a need for in silico approaches to facilitate the analysis of these data. Here, we present a detailed model of mitochondrial metabolism Saccharomyces cerevisiae, including protein import, iron-sulfur cluster biosynthesis, and a description of the coupling between charge translocation processes and ATP synthesis. Model analysis implied a dual dependence of absolute levels of proteins in protein import, iron-sulfur cluster biogenesis and cluster abundance on growth rate and respiratory activity. The model is instrumental in studying dynamics and perturbations in these processes and given the high conservation of mitochondrial metabolism in humans, it can provide insight into their role in human disease.Carl MalinaFrancesca Di BartolomeoEduard J. KerkhovenJens NielsenElsevierarticleCellular physiologyCell biologyIntegrative aspects of cell biologySystems biologyIn silico biologyScienceQENiScience, Vol 24, Iss 11, Pp 103294- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cellular physiology Cell biology Integrative aspects of cell biology Systems biology In silico biology Science Q
spellingShingle	Cellular physiology Cell biology Integrative aspects of cell biology Systems biology In silico biology Science Q Carl Malina Francesca Di Bartolomeo Eduard J. Kerkhoven Jens Nielsen Constraint-based modeling of yeast mitochondria reveals the dynamics of protein import and iron-sulfur cluster biogenesis
description	Summary: Mitochondria are a hallmark of eukaryal cells and play an important role in cellular metabolism. There is a vast amount of knowledge available on mitochondrial metabolism and essential mitochondrial functions, such as protein import and iron-sulfur cluster biosynthesis, including multiple studies on the mitochondrial proteome. Therefore, there is a need for in silico approaches to facilitate the analysis of these data. Here, we present a detailed model of mitochondrial metabolism Saccharomyces cerevisiae, including protein import, iron-sulfur cluster biosynthesis, and a description of the coupling between charge translocation processes and ATP synthesis. Model analysis implied a dual dependence of absolute levels of proteins in protein import, iron-sulfur cluster biogenesis and cluster abundance on growth rate and respiratory activity. The model is instrumental in studying dynamics and perturbations in these processes and given the high conservation of mitochondrial metabolism in humans, it can provide insight into their role in human disease.
format	article
author	Carl Malina Francesca Di Bartolomeo Eduard J. Kerkhoven Jens Nielsen
author_facet	Carl Malina Francesca Di Bartolomeo Eduard J. Kerkhoven Jens Nielsen
author_sort	Carl Malina
title	Constraint-based modeling of yeast mitochondria reveals the dynamics of protein import and iron-sulfur cluster biogenesis
title_short	Constraint-based modeling of yeast mitochondria reveals the dynamics of protein import and iron-sulfur cluster biogenesis
title_full	Constraint-based modeling of yeast mitochondria reveals the dynamics of protein import and iron-sulfur cluster biogenesis
title_fullStr	Constraint-based modeling of yeast mitochondria reveals the dynamics of protein import and iron-sulfur cluster biogenesis
title_full_unstemmed	Constraint-based modeling of yeast mitochondria reveals the dynamics of protein import and iron-sulfur cluster biogenesis
title_sort	constraint-based modeling of yeast mitochondria reveals the dynamics of protein import and iron-sulfur cluster biogenesis
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/a5f29949808a4ea5b5bf307bdf677aaf
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Constraint-based modeling of yeast mitochondria reveals the dynamics of protein import and iron-sulfur cluster biogenesis

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