Trabecular meshwork gene expression after selective laser trabeculoplasty.

<h4>Background</h4>Trabecular meshwork and Schlemm's canal are the tissues appointed to modulate the aqueous humour outflow from the anterior chamber. The impairment of their functions drives to an intraocular pressure increase. The selective laser trabeculoplasty is a laser therapy...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alberto Izzotti, Mariagrazia Longobardi, Cristina Cartiglia, Federico Rathschuler, Sergio Claudio Saccà
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a5f72884d2cb4a819da21b1cf2ef1ed3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a5f72884d2cb4a819da21b1cf2ef1ed3
record_format	dspace
spelling	oai:doaj.org-article:a5f72884d2cb4a819da21b1cf2ef1ed32021-11-18T06:50:51ZTrabecular meshwork gene expression after selective laser trabeculoplasty.1932-620310.1371/journal.pone.0020110https://doaj.org/article/a5f72884d2cb4a819da21b1cf2ef1ed32011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21747927/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Trabecular meshwork and Schlemm's canal are the tissues appointed to modulate the aqueous humour outflow from the anterior chamber. The impairment of their functions drives to an intraocular pressure increase. The selective laser trabeculoplasty is a laser therapy of the trabecular meshwork able to decrease intraocular pressure. The exact response mechanism to this treatment has not been clearly delineated yet. The herein presented study is aimed at studying the gene expression changes induced in trabecular meshwork cells by selective laser trabeculoplasty (SLT) in order to better understand the mechanisms subtending its efficacy.<h4>Methodology/principal findings</h4>Primary human trabecular meshwork cells cultured in fibroblast medium underwent selective laser trabeculoplasty treatment. RNA was extracted from a pool of cells 30 minutes after treatment while the remaining cells were further cultured and RNA was extracted respectively 2 and 6 hours after treatment. Control cells stored in incubator in absence of SLT treatment were used as reference samples. Gene expression was evaluated by hybridization on miRNA-microarray and laser scanner analysis. Scanning electron microscopic examination was performed on 2 Trabecular meshwork samples after SLT at 4(th) and 6(th) hour from treatment. On the whole, selective laser trabeculoplasty modulates in trabecular meshwork the expression of genes involved in cell motility, intercellular connections, extracellular matrix production, protein repair, DNA repair, membrane repair, reactive oxygen species production, glutamate toxicity, antioxidant activities, and inflammation.<h4>Conclusions/significance</h4>SLT did not induce any phenotypic alteration in TM samples. TM is a complex tissue possessing a great variety of function pivotal for the active regulation of aqueous humour outflow from the anterior chamber. SLT is able to modulate these functions at the postgenomic molecular level without inducing damage either at molecular or phenotypic levels.Alberto IzzottiMariagrazia LongobardiCristina CartigliaFederico RathschulerSergio Claudio SaccàPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e20110 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alberto Izzotti Mariagrazia Longobardi Cristina Cartiglia Federico Rathschuler Sergio Claudio Saccà Trabecular meshwork gene expression after selective laser trabeculoplasty.
description	<h4>Background</h4>Trabecular meshwork and Schlemm's canal are the tissues appointed to modulate the aqueous humour outflow from the anterior chamber. The impairment of their functions drives to an intraocular pressure increase. The selective laser trabeculoplasty is a laser therapy of the trabecular meshwork able to decrease intraocular pressure. The exact response mechanism to this treatment has not been clearly delineated yet. The herein presented study is aimed at studying the gene expression changes induced in trabecular meshwork cells by selective laser trabeculoplasty (SLT) in order to better understand the mechanisms subtending its efficacy.<h4>Methodology/principal findings</h4>Primary human trabecular meshwork cells cultured in fibroblast medium underwent selective laser trabeculoplasty treatment. RNA was extracted from a pool of cells 30 minutes after treatment while the remaining cells were further cultured and RNA was extracted respectively 2 and 6 hours after treatment. Control cells stored in incubator in absence of SLT treatment were used as reference samples. Gene expression was evaluated by hybridization on miRNA-microarray and laser scanner analysis. Scanning electron microscopic examination was performed on 2 Trabecular meshwork samples after SLT at 4(th) and 6(th) hour from treatment. On the whole, selective laser trabeculoplasty modulates in trabecular meshwork the expression of genes involved in cell motility, intercellular connections, extracellular matrix production, protein repair, DNA repair, membrane repair, reactive oxygen species production, glutamate toxicity, antioxidant activities, and inflammation.<h4>Conclusions/significance</h4>SLT did not induce any phenotypic alteration in TM samples. TM is a complex tissue possessing a great variety of function pivotal for the active regulation of aqueous humour outflow from the anterior chamber. SLT is able to modulate these functions at the postgenomic molecular level without inducing damage either at molecular or phenotypic levels.
format	article
author	Alberto Izzotti Mariagrazia Longobardi Cristina Cartiglia Federico Rathschuler Sergio Claudio Saccà
author_facet	Alberto Izzotti Mariagrazia Longobardi Cristina Cartiglia Federico Rathschuler Sergio Claudio Saccà
author_sort	Alberto Izzotti
title	Trabecular meshwork gene expression after selective laser trabeculoplasty.
title_short	Trabecular meshwork gene expression after selective laser trabeculoplasty.
title_full	Trabecular meshwork gene expression after selective laser trabeculoplasty.
title_fullStr	Trabecular meshwork gene expression after selective laser trabeculoplasty.
title_full_unstemmed	Trabecular meshwork gene expression after selective laser trabeculoplasty.
title_sort	trabecular meshwork gene expression after selective laser trabeculoplasty.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a5f72884d2cb4a819da21b1cf2ef1ed3
work_keys_str_mv	AT albertoizzotti trabecularmeshworkgeneexpressionafterselectivelasertrabeculoplasty AT mariagrazialongobardi trabecularmeshworkgeneexpressionafterselectivelasertrabeculoplasty AT cristinacartiglia trabecularmeshworkgeneexpressionafterselectivelasertrabeculoplasty AT federicorathschuler trabecularmeshworkgeneexpressionafterselectivelasertrabeculoplasty AT sergioclaudiosacca trabecularmeshworkgeneexpressionafterselectivelasertrabeculoplasty
_version_	1718424311284367360

Trabecular meshwork gene expression after selective laser trabeculoplasty.

Ejemplares similares