Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i>

Many plant proteins with extracellular leucine-rich repeat (eLRR) domains play an important role in plant immunity. However, the role of one class of eLRR plant proteins—the simple eLRR proteins—in plant defenses against herbivores remains largely unknown. Here, we found that a simple eLRR protein O...

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Autores principales:	Miaofen Ye, Peng Kuai, Shuting Chen, Na Lin, Meng Ye, Lingfei Hu, Yonggen Lou
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	rice <i>OsI-BAK1</i> <i>Nilaparvata lugens</i> ethylene plant defense responses simple extracellular leucine-rich repeat protein Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/a5fa5d25ca144a8691b56a8288ec4d0f
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spelling	oai:doaj.org-article:a5fa5d25ca144a8691b56a8288ec4d0f2021-11-25T17:54:05ZSilencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i>10.3390/ijms2222121821422-00671661-6596https://doaj.org/article/a5fa5d25ca144a8691b56a8288ec4d0f2021-11-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/22/12182https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067Many plant proteins with extracellular leucine-rich repeat (eLRR) domains play an important role in plant immunity. However, the role of one class of eLRR plant proteins—the simple eLRR proteins—in plant defenses against herbivores remains largely unknown. Here, we found that a simple eLRR protein OsI-BAK1 in rice localizes to the plasma membrane. Its expression was induced by mechanical wounding, the infestation of gravid females of brown planthopper (BPH) <i>Nilaparvata lugens</i> or white-backed planthopper <i>Sogatella furcifera</i> and treatment with methyl jasmonate or abscisic acid. Silencing <i>OsI-BAK1</i> (ir-<i>ibak1</i>) in rice enhanced the BPH-induced transcript levels of three defense-related WRKY genes (<i>OsWRKY24</i>, <i>OsWRKY53</i> and <i>OsWRKY70</i>) but decreased the induced levels of ethylene. Bioassays revealed that the hatching rate was significantly lower in BPH eggs laid on ir-<i>ibak1</i> plants than wild-type (WT) plants; moreover, gravid BPH females preferred to oviposit on WT plants over ir-<i>ibak1</i> plants. The exogenous application of ethephon on ir-<i>ibak1</i> plants eliminated the BPH oviposition preference between WT and ir-<i>ibak1</i> plants but had no effect on the hatching rate of BPH eggs. These findings suggest that OsI-BAK1 acts as a negative modulator of defense responses in rice to BPH and that BPH might exploit this modulator for its own benefit.Miaofen YePeng KuaiShuting ChenNa LinMeng YeLingfei HuYonggen LouMDPI AGarticlerice<i>OsI-BAK1</i><i>Nilaparvata lugens</i>ethyleneplant defense responsessimple extracellular leucine-rich repeat proteinBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 12182, p 12182 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rice <i>OsI-BAK1</i> <i>Nilaparvata lugens</i> ethylene plant defense responses simple extracellular leucine-rich repeat protein Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	rice <i>OsI-BAK1</i> <i>Nilaparvata lugens</i> ethylene plant defense responses simple extracellular leucine-rich repeat protein Biology (General) QH301-705.5 Chemistry QD1-999 Miaofen Ye Peng Kuai Shuting Chen Na Lin Meng Ye Lingfei Hu Yonggen Lou Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i>
description	Many plant proteins with extracellular leucine-rich repeat (eLRR) domains play an important role in plant immunity. However, the role of one class of eLRR plant proteins—the simple eLRR proteins—in plant defenses against herbivores remains largely unknown. Here, we found that a simple eLRR protein OsI-BAK1 in rice localizes to the plasma membrane. Its expression was induced by mechanical wounding, the infestation of gravid females of brown planthopper (BPH) <i>Nilaparvata lugens</i> or white-backed planthopper <i>Sogatella furcifera</i> and treatment with methyl jasmonate or abscisic acid. Silencing <i>OsI-BAK1</i> (ir-<i>ibak1</i>) in rice enhanced the BPH-induced transcript levels of three defense-related WRKY genes (<i>OsWRKY24</i>, <i>OsWRKY53</i> and <i>OsWRKY70</i>) but decreased the induced levels of ethylene. Bioassays revealed that the hatching rate was significantly lower in BPH eggs laid on ir-<i>ibak1</i> plants than wild-type (WT) plants; moreover, gravid BPH females preferred to oviposit on WT plants over ir-<i>ibak1</i> plants. The exogenous application of ethephon on ir-<i>ibak1</i> plants eliminated the BPH oviposition preference between WT and ir-<i>ibak1</i> plants but had no effect on the hatching rate of BPH eggs. These findings suggest that OsI-BAK1 acts as a negative modulator of defense responses in rice to BPH and that BPH might exploit this modulator for its own benefit.
format	article
author	Miaofen Ye Peng Kuai Shuting Chen Na Lin Meng Ye Lingfei Hu Yonggen Lou
author_facet	Miaofen Ye Peng Kuai Shuting Chen Na Lin Meng Ye Lingfei Hu Yonggen Lou
author_sort	Miaofen Ye
title	Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i>
title_short	Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i>
title_full	Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i>
title_fullStr	Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i>
title_full_unstemmed	Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i>
title_sort	silencing a simple extracellular leucine-rich repeat gene <i>osi-bak1</i> enhances the resistance of rice to brown planthopper <i>nilaparvata lugens</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a5fa5d25ca144a8691b56a8288ec4d0f
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Silencing a Simple Extracellular Leucine-Rich Repeat Gene <i>OsI-BAK1</i> Enhances the Resistance of Rice to Brown Planthopper <i>Nilaparvata lugens</i>

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