Non-specific interactions of antibody-oligonucleotide conjugates with living cells

Abstract Antibody-Oligonucleotide Conjugates (AOCs) represent an emerging class of functionalized antibodies that have already been used in a wide variety of applications. While the impact of dye and drug conjugation on antibodies’ ability to bind their target has been extensively studied, little is...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Victor Lehot, Isabelle Kuhn, Marc Nothisen, Stéphane Erb, Sergii Kolodych, Sarah Cianférani, Guilhem Chaubet, Alain Wagner
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a5fbede99bbf44099b9275daa73200ad
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a5fbede99bbf44099b9275daa73200ad
record_format	dspace
spelling	oai:doaj.org-article:a5fbede99bbf44099b9275daa73200ad2021-12-02T11:39:47ZNon-specific interactions of antibody-oligonucleotide conjugates with living cells10.1038/s41598-021-85352-w2045-2322https://doaj.org/article/a5fbede99bbf44099b9275daa73200ad2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-85352-whttps://doaj.org/toc/2045-2322Abstract Antibody-Oligonucleotide Conjugates (AOCs) represent an emerging class of functionalized antibodies that have already been used in a wide variety of applications. While the impact of dye and drug conjugation on antibodies’ ability to bind their target has been extensively studied, little is known about the effect caused by the conjugation of hydrophilic and charged payloads such as oligonucleotides on the functions of an antibody. Previous observations of non-specific interactions of nucleic acids with untargeted cells prompted us to further investigate their impact on AOC binding abilities and cell selectivity. We synthesized a series of single- and double-stranded AOCs, as well as a human serum albumin-oligonucleotide conjugate, and studied their interactions with both targeted and non-targeted living cells using a time-resolved analysis of ligand binding assay. Our results indicate that conjugation of single strand oligonucleotides to proteins induce consistent non-specific interactions with cell surfaces while double strand oligonucleotides have little or no effect, depending on the preparation method.Victor LehotIsabelle KuhnMarc NothisenStéphane ErbSergii KolodychSarah CianféraniGuilhem ChaubetAlain WagnerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Victor Lehot Isabelle Kuhn Marc Nothisen Stéphane Erb Sergii Kolodych Sarah Cianférani Guilhem Chaubet Alain Wagner Non-specific interactions of antibody-oligonucleotide conjugates with living cells
description	Abstract Antibody-Oligonucleotide Conjugates (AOCs) represent an emerging class of functionalized antibodies that have already been used in a wide variety of applications. While the impact of dye and drug conjugation on antibodies’ ability to bind their target has been extensively studied, little is known about the effect caused by the conjugation of hydrophilic and charged payloads such as oligonucleotides on the functions of an antibody. Previous observations of non-specific interactions of nucleic acids with untargeted cells prompted us to further investigate their impact on AOC binding abilities and cell selectivity. We synthesized a series of single- and double-stranded AOCs, as well as a human serum albumin-oligonucleotide conjugate, and studied their interactions with both targeted and non-targeted living cells using a time-resolved analysis of ligand binding assay. Our results indicate that conjugation of single strand oligonucleotides to proteins induce consistent non-specific interactions with cell surfaces while double strand oligonucleotides have little or no effect, depending on the preparation method.
format	article
author	Victor Lehot Isabelle Kuhn Marc Nothisen Stéphane Erb Sergii Kolodych Sarah Cianférani Guilhem Chaubet Alain Wagner
author_facet	Victor Lehot Isabelle Kuhn Marc Nothisen Stéphane Erb Sergii Kolodych Sarah Cianférani Guilhem Chaubet Alain Wagner
author_sort	Victor Lehot
title	Non-specific interactions of antibody-oligonucleotide conjugates with living cells
title_short	Non-specific interactions of antibody-oligonucleotide conjugates with living cells
title_full	Non-specific interactions of antibody-oligonucleotide conjugates with living cells
title_fullStr	Non-specific interactions of antibody-oligonucleotide conjugates with living cells
title_full_unstemmed	Non-specific interactions of antibody-oligonucleotide conjugates with living cells
title_sort	non-specific interactions of antibody-oligonucleotide conjugates with living cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a5fbede99bbf44099b9275daa73200ad
work_keys_str_mv	AT victorlehot nonspecificinteractionsofantibodyoligonucleotideconjugateswithlivingcells AT isabellekuhn nonspecificinteractionsofantibodyoligonucleotideconjugateswithlivingcells AT marcnothisen nonspecificinteractionsofantibodyoligonucleotideconjugateswithlivingcells AT stephaneerb nonspecificinteractionsofantibodyoligonucleotideconjugateswithlivingcells AT sergiikolodych nonspecificinteractionsofantibodyoligonucleotideconjugateswithlivingcells AT sarahcianferani nonspecificinteractionsofantibodyoligonucleotideconjugateswithlivingcells AT guilhemchaubet nonspecificinteractionsofantibodyoligonucleotideconjugateswithlivingcells AT alainwagner nonspecificinteractionsofantibodyoligonucleotideconjugateswithlivingcells
_version_	1718395717883527168

Non-specific interactions of antibody-oligonucleotide conjugates with living cells

Ejemplares similares