Identification of allergens for food-dependent exercise-induced anaphylaxis to shrimp

Abstract Shrimp is a causative food that elicits food-dependent exercise-induced anaphylaxis (FDEIA). In this study, we sought to identify IgE-binding allergens in patients with shrimp-FDEIA. Sera were obtained from eight patients with shrimp-FDEIA and two healthy control subjects. Proteins were ext...

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Autores principales:	Shiori Akimoto, Tomoharu Yokooji, Ryohei Ogino, Yuko Chinuki, Takanori Taogoshi, Atsuko Adachi, Eishin Morita, Hiroaki Matsuo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a5fdc7b7833243d082aeeaa2d4656511
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spelling	oai:doaj.org-article:a5fdc7b7833243d082aeeaa2d46565112021-12-02T15:52:59ZIdentification of allergens for food-dependent exercise-induced anaphylaxis to shrimp10.1038/s41598-021-84752-22045-2322https://doaj.org/article/a5fdc7b7833243d082aeeaa2d46565112021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84752-2https://doaj.org/toc/2045-2322Abstract Shrimp is a causative food that elicits food-dependent exercise-induced anaphylaxis (FDEIA). In this study, we sought to identify IgE-binding allergens in patients with shrimp-FDEIA. Sera were obtained from eight patients with shrimp-FDEIA and two healthy control subjects. Proteins were extracted from four shrimp species by homogenization in Tris buffer. Immunoblot analysis revealed that IgE from patient sera bound strongly to a 70-kDa and a 43-kDa protein in a preparation of Tris-soluble extracts from Litopenaeus vannamei. Mass spectrometry identified the 70-kDa and 43-kDa proteins as a P75 homologue and fructose 1,6-bisphosphate aldolase (FBPA), respectively. To confirm that the putative shrimp allergens were specifically recognized by serum IgE from shrimp-FDEIA patients, the two proteins were purified by ammonium sulfate precipitation followed by reversed-phase HPLC and/or anion-exchange hydrophobic interaction chromatography and then subjected to immunoblot analysis. Purified P75 homologue and FBPA were positively bound by serum IgE from one and three, respectively, of the eight patients with shrimp-FDEIA, but not by sera from control subjects. Thus, P75 homologue and FBPA are identified as IgE-binding allergens for shrimp-FDEIA. These findings could be useful for the development of diagnostic tools and desensitization therapy for shrimp-FDEIA patients.Shiori AkimotoTomoharu YokoojiRyohei OginoYuko ChinukiTakanori TaogoshiAtsuko AdachiEishin MoritaHiroaki MatsuoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shiori Akimoto Tomoharu Yokooji Ryohei Ogino Yuko Chinuki Takanori Taogoshi Atsuko Adachi Eishin Morita Hiroaki Matsuo Identification of allergens for food-dependent exercise-induced anaphylaxis to shrimp
description	Abstract Shrimp is a causative food that elicits food-dependent exercise-induced anaphylaxis (FDEIA). In this study, we sought to identify IgE-binding allergens in patients with shrimp-FDEIA. Sera were obtained from eight patients with shrimp-FDEIA and two healthy control subjects. Proteins were extracted from four shrimp species by homogenization in Tris buffer. Immunoblot analysis revealed that IgE from patient sera bound strongly to a 70-kDa and a 43-kDa protein in a preparation of Tris-soluble extracts from Litopenaeus vannamei. Mass spectrometry identified the 70-kDa and 43-kDa proteins as a P75 homologue and fructose 1,6-bisphosphate aldolase (FBPA), respectively. To confirm that the putative shrimp allergens were specifically recognized by serum IgE from shrimp-FDEIA patients, the two proteins were purified by ammonium sulfate precipitation followed by reversed-phase HPLC and/or anion-exchange hydrophobic interaction chromatography and then subjected to immunoblot analysis. Purified P75 homologue and FBPA were positively bound by serum IgE from one and three, respectively, of the eight patients with shrimp-FDEIA, but not by sera from control subjects. Thus, P75 homologue and FBPA are identified as IgE-binding allergens for shrimp-FDEIA. These findings could be useful for the development of diagnostic tools and desensitization therapy for shrimp-FDEIA patients.
format	article
author	Shiori Akimoto Tomoharu Yokooji Ryohei Ogino Yuko Chinuki Takanori Taogoshi Atsuko Adachi Eishin Morita Hiroaki Matsuo
author_facet	Shiori Akimoto Tomoharu Yokooji Ryohei Ogino Yuko Chinuki Takanori Taogoshi Atsuko Adachi Eishin Morita Hiroaki Matsuo
author_sort	Shiori Akimoto
title	Identification of allergens for food-dependent exercise-induced anaphylaxis to shrimp
title_short	Identification of allergens for food-dependent exercise-induced anaphylaxis to shrimp
title_full	Identification of allergens for food-dependent exercise-induced anaphylaxis to shrimp
title_fullStr	Identification of allergens for food-dependent exercise-induced anaphylaxis to shrimp
title_full_unstemmed	Identification of allergens for food-dependent exercise-induced anaphylaxis to shrimp
title_sort	identification of allergens for food-dependent exercise-induced anaphylaxis to shrimp
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a5fdc7b7833243d082aeeaa2d4656511
work_keys_str_mv	AT shioriakimoto identificationofallergensforfooddependentexerciseinducedanaphylaxistoshrimp AT tomoharuyokooji identificationofallergensforfooddependentexerciseinducedanaphylaxistoshrimp AT ryoheiogino identificationofallergensforfooddependentexerciseinducedanaphylaxistoshrimp AT yukochinuki identificationofallergensforfooddependentexerciseinducedanaphylaxistoshrimp AT takanoritaogoshi identificationofallergensforfooddependentexerciseinducedanaphylaxistoshrimp AT atsukoadachi identificationofallergensforfooddependentexerciseinducedanaphylaxistoshrimp AT eishinmorita identificationofallergensforfooddependentexerciseinducedanaphylaxistoshrimp AT hiroakimatsuo identificationofallergensforfooddependentexerciseinducedanaphylaxistoshrimp
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Identification of allergens for food-dependent exercise-induced anaphylaxis to shrimp

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