3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering

Liver-associated diseases and tissue engineering approaches based on in vitro culture of functional Primary human hepatocytes (PHH) had been restricted by the rapid de-differentiation in 2D culture conditions which restricted their usability. It was proven that cells growing in 3D format can better...

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Autores principales:	Amit Panwar, Prativa Das, Lay Poh Tan
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	liver tissue engineering hepatic organoids hepatic function hepatocytes 3D culture Technology T Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a602ace351044574b942e5322d2efe0f
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spelling	oai:doaj.org-article:a602ace351044574b942e5322d2efe0f2021-11-25T16:46:40Z3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering10.3390/bioengineering81101852306-5354https://doaj.org/article/a602ace351044574b942e5322d2efe0f2021-11-01T00:00:00Zhttps://www.mdpi.com/2306-5354/8/11/185https://doaj.org/toc/2306-5354Liver-associated diseases and tissue engineering approaches based on in vitro culture of functional Primary human hepatocytes (PHH) had been restricted by the rapid de-differentiation in 2D culture conditions which restricted their usability. It was proven that cells growing in 3D format can better mimic the in vivo microenvironment, and thus help in maintaining metabolic activity, phenotypic properties, and longevity of the in vitro cultures. Again, the culture method and type of cell population are also recognized as important parameters for functional maintenance of primary hepatocytes. Hepatic organoids formed by self-assembly of hepatic cells are microtissues, and were able to show long-term in vitro maintenance of hepato-specific characteristics. Thus, hepatic organoids were recognized as an effective tool for screening potential cures and modeling liver diseases effectively. The current review summarizes the importance of 3D hepatic organoid culture over other conventional 2D and 3D culture models and its applicability in Liver tissue engineering.Amit PanwarPrativa DasLay Poh TanMDPI AGarticleliver tissue engineeringhepatic organoidshepatic functionhepatocytes3D cultureTechnologyTBiology (General)QH301-705.5ENBioengineering, Vol 8, Iss 185, p 185 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	liver tissue engineering hepatic organoids hepatic function hepatocytes 3D culture Technology T Biology (General) QH301-705.5
spellingShingle	liver tissue engineering hepatic organoids hepatic function hepatocytes 3D culture Technology T Biology (General) QH301-705.5 Amit Panwar Prativa Das Lay Poh Tan 3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering
description	Liver-associated diseases and tissue engineering approaches based on in vitro culture of functional Primary human hepatocytes (PHH) had been restricted by the rapid de-differentiation in 2D culture conditions which restricted their usability. It was proven that cells growing in 3D format can better mimic the in vivo microenvironment, and thus help in maintaining metabolic activity, phenotypic properties, and longevity of the in vitro cultures. Again, the culture method and type of cell population are also recognized as important parameters for functional maintenance of primary hepatocytes. Hepatic organoids formed by self-assembly of hepatic cells are microtissues, and were able to show long-term in vitro maintenance of hepato-specific characteristics. Thus, hepatic organoids were recognized as an effective tool for screening potential cures and modeling liver diseases effectively. The current review summarizes the importance of 3D hepatic organoid culture over other conventional 2D and 3D culture models and its applicability in Liver tissue engineering.
format	article
author	Amit Panwar Prativa Das Lay Poh Tan
author_facet	Amit Panwar Prativa Das Lay Poh Tan
author_sort	Amit Panwar
title	3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering
title_short	3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering
title_full	3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering
title_fullStr	3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering
title_full_unstemmed	3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering
title_sort	3d hepatic organoid-based advancements in liver tissue engineering
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a602ace351044574b942e5322d2efe0f
work_keys_str_mv	AT amitpanwar 3dhepaticorganoidbasedadvancementsinlivertissueengineering AT prativadas 3dhepaticorganoidbasedadvancementsinlivertissueengineering AT laypohtan 3dhepaticorganoidbasedadvancementsinlivertissueengineering
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3D Hepatic Organoid-Based Advancements in LIVER Tissue Engineering

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