Some conditions apply: Systems for studying Plasmodium falciparum protein function.

Malaria, caused by infection with Plasmodium parasites, remains a significant global health concern. For decades, genetic intractability and limited tools hindered our ability to study essential proteins and pathways in Plasmodium falciparum, the parasite associated with the most severe malaria case...

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Autores principales:	Heather M Kudyba, David W Cobb, Joel Vega-Rodríguez, Vasant Muralidharan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a60a9861235342c0b60f5bedb84549c5
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spelling	oai:doaj.org-article:a60a9861235342c0b60f5bedb84549c52021-12-02T19:59:37ZSome conditions apply: Systems for studying Plasmodium falciparum protein function.1553-73661553-737410.1371/journal.ppat.1009442https://doaj.org/article/a60a9861235342c0b60f5bedb84549c52021-04-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1009442https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Malaria, caused by infection with Plasmodium parasites, remains a significant global health concern. For decades, genetic intractability and limited tools hindered our ability to study essential proteins and pathways in Plasmodium falciparum, the parasite associated with the most severe malaria cases. However, recent years have seen major leaps forward in the ability to genetically manipulate P. falciparum parasites and conditionally control protein expression/function. The conditional knockdown systems used in P. falciparum target all 3 components of the central dogma, allowing researchers to conditionally control gene expression, translation, and protein function. Here, we review some of the common knockdown systems that have been adapted or developed for use in P. falciparum. Much of the work done using conditional knockdown approaches has been performed in asexual, blood-stage parasites, but we also highlight their uses in other parts of the life cycle and discuss new ways of applying these systems outside of the intraerythrocytic stages. With the use of these tools, the field's understanding of parasite biology is ever increasing, and promising new pathways for antimalarial drug development are being discovered.Heather M KudybaDavid W CobbJoel Vega-RodríguezVasant MuralidharanPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 4, p e1009442 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Heather M Kudyba David W Cobb Joel Vega-Rodríguez Vasant Muralidharan Some conditions apply: Systems for studying Plasmodium falciparum protein function.
description	Malaria, caused by infection with Plasmodium parasites, remains a significant global health concern. For decades, genetic intractability and limited tools hindered our ability to study essential proteins and pathways in Plasmodium falciparum, the parasite associated with the most severe malaria cases. However, recent years have seen major leaps forward in the ability to genetically manipulate P. falciparum parasites and conditionally control protein expression/function. The conditional knockdown systems used in P. falciparum target all 3 components of the central dogma, allowing researchers to conditionally control gene expression, translation, and protein function. Here, we review some of the common knockdown systems that have been adapted or developed for use in P. falciparum. Much of the work done using conditional knockdown approaches has been performed in asexual, blood-stage parasites, but we also highlight their uses in other parts of the life cycle and discuss new ways of applying these systems outside of the intraerythrocytic stages. With the use of these tools, the field's understanding of parasite biology is ever increasing, and promising new pathways for antimalarial drug development are being discovered.
format	article
author	Heather M Kudyba David W Cobb Joel Vega-Rodríguez Vasant Muralidharan
author_facet	Heather M Kudyba David W Cobb Joel Vega-Rodríguez Vasant Muralidharan
author_sort	Heather M Kudyba
title	Some conditions apply: Systems for studying Plasmodium falciparum protein function.
title_short	Some conditions apply: Systems for studying Plasmodium falciparum protein function.
title_full	Some conditions apply: Systems for studying Plasmodium falciparum protein function.
title_fullStr	Some conditions apply: Systems for studying Plasmodium falciparum protein function.
title_full_unstemmed	Some conditions apply: Systems for studying Plasmodium falciparum protein function.
title_sort	some conditions apply: systems for studying plasmodium falciparum protein function.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a60a9861235342c0b60f5bedb84549c5
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Some conditions apply: Systems for studying Plasmodium falciparum protein function.

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