Distribution of genetic markers at workers of aluminum industry with professional fluorosis: serum proteins and erythrocyte isoenzymes

The aim of this work was the study of the association of biochemical markers of genes with the risk of the development of professional and work-related fluorosis in people, working in the aluminum industry. From 303 to 387 employees of Novokuznetsk aluminum factory were examined for various polymorp...

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Autor principal:	N. I. Gafarov
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2013
Materias:	fluorosis genetic markers of resistance and susceptibility Science Q
Acceso en línea:	https://doaj.org/article/a60ce68722b248afb972e85da9348f03
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spelling	oai:doaj.org-article:a60ce68722b248afb972e85da9348f032021-11-23T06:14:27ZDistribution of genetic markers at workers of aluminum industry with professional fluorosis: serum proteins and erythrocyte isoenzymes2541-94202587-9596https://doaj.org/article/a60ce68722b248afb972e85da9348f032013-05-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/1488https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596The aim of this work was the study of the association of biochemical markers of genes with the risk of the development of professional and work-related fluorosis in people, working in the aluminum industry. From 303 to 387 employees of Novokuznetsk aluminum factory were examined for various polymorphic genetic systems. The main group consisted of patients with fluorosis who were tested in the clinic of Research Institute of Complex Problems of Hygiene and Occupational Diseases RAMS. The control group (110 people) consisted of apparently healthy individuals of the same professions, working on Novokuznetsk aluminum factory, but without a diagnosis of occupational disease. We studied the serum proteins haptoglobin (HP), group-specific component GC, erythrocyte acid phosphatase ACP, fluorescent esterase EsD, protease inhibitor a-1-antitrypsin AAT. The material for the study was the venous blood samples. The study was conducted with the use of electrophoresis in polyacrylamide and the starch gels followed by histochemical staining electrophoregrams. Variants of protease inhibitor a-1-antitrypsin (AAT) were separated by isoelectric focusing in polyacrylamide gel. For analyzing the association studies of gene markers of the risk of develop ment (or resistance to the development) of fluorosis the criteria χ2 and OR were used. It was revealed that variants AcP aa, AcP bb, AcP bc, EsD 2-2, PI M3M3 and GC, combinations (1-1) + PI (MR), GC (11) + PI (M3M3), PI ( MR) + EsD (2-2), GC (1-1) + PI (M2M3), EsD (2-2) + AcP aa, GC (1-1) + EsD (2-2) referred to the risk genotypes of development of fluorosis. Two-component combinations of genotypes that were also associated with an increased risk of development of professional fluorosis were identified: GC (1-1) + PI (MR), GC (1-1) + PI (M3M3), PI (MR) + EsD (2-2 ); GC (1-1) + PI (M2M3), EsD (2-2) + AcP aa; GC (1-1) + EsD (2-2). For three-way combinations of genotypes are GC (1-1) + PI (MR) + AcP AA and GC (1-1) + EsD (2-2) + AcP cc. Genotypes resistant variants are: PI M1M1, EsD 1-1, AcP ab, AcP ac.N. I. GafarovScientific Сentre for Family Health and Human Reproduction Problemsarticlefluorosisgenetic markers of resistance and susceptibilityScienceQRUActa Biomedica Scientifica, Vol 0, Iss 3(2), Pp 48-52 (2013)
institution	DOAJ
collection	DOAJ
language	RU
topic	fluorosis genetic markers of resistance and susceptibility Science Q
spellingShingle	fluorosis genetic markers of resistance and susceptibility Science Q N. I. Gafarov Distribution of genetic markers at workers of aluminum industry with professional fluorosis: serum proteins and erythrocyte isoenzymes
description	The aim of this work was the study of the association of biochemical markers of genes with the risk of the development of professional and work-related fluorosis in people, working in the aluminum industry. From 303 to 387 employees of Novokuznetsk aluminum factory were examined for various polymorphic genetic systems. The main group consisted of patients with fluorosis who were tested in the clinic of Research Institute of Complex Problems of Hygiene and Occupational Diseases RAMS. The control group (110 people) consisted of apparently healthy individuals of the same professions, working on Novokuznetsk aluminum factory, but without a diagnosis of occupational disease. We studied the serum proteins haptoglobin (HP), group-specific component GC, erythrocyte acid phosphatase ACP, fluorescent esterase EsD, protease inhibitor a-1-antitrypsin AAT. The material for the study was the venous blood samples. The study was conducted with the use of electrophoresis in polyacrylamide and the starch gels followed by histochemical staining electrophoregrams. Variants of protease inhibitor a-1-antitrypsin (AAT) were separated by isoelectric focusing in polyacrylamide gel. For analyzing the association studies of gene markers of the risk of develop ment (or resistance to the development) of fluorosis the criteria χ2 and OR were used. It was revealed that variants AcP aa, AcP bb, AcP bc, EsD 2-2, PI M3M3 and GC, combinations (1-1) + PI (MR), GC (11) + PI (M3M3), PI ( MR) + EsD (2-2), GC (1-1) + PI (M2M3), EsD (2-2) + AcP aa, GC (1-1) + EsD (2-2) referred to the risk genotypes of development of fluorosis. Two-component combinations of genotypes that were also associated with an increased risk of development of professional fluorosis were identified: GC (1-1) + PI (MR), GC (1-1) + PI (M3M3), PI (MR) + EsD (2-2 ); GC (1-1) + PI (M2M3), EsD (2-2) + AcP aa; GC (1-1) + EsD (2-2). For three-way combinations of genotypes are GC (1-1) + PI (MR) + AcP AA and GC (1-1) + EsD (2-2) + AcP cc. Genotypes resistant variants are: PI M1M1, EsD 1-1, AcP ab, AcP ac.
format	article
author	N. I. Gafarov
author_facet	N. I. Gafarov
author_sort	N. I. Gafarov
title	Distribution of genetic markers at workers of aluminum industry with professional fluorosis: serum proteins and erythrocyte isoenzymes
title_short	Distribution of genetic markers at workers of aluminum industry with professional fluorosis: serum proteins and erythrocyte isoenzymes
title_full	Distribution of genetic markers at workers of aluminum industry with professional fluorosis: serum proteins and erythrocyte isoenzymes
title_fullStr	Distribution of genetic markers at workers of aluminum industry with professional fluorosis: serum proteins and erythrocyte isoenzymes
title_full_unstemmed	Distribution of genetic markers at workers of aluminum industry with professional fluorosis: serum proteins and erythrocyte isoenzymes
title_sort	distribution of genetic markers at workers of aluminum industry with professional fluorosis: serum proteins and erythrocyte isoenzymes
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2013
url	https://doaj.org/article/a60ce68722b248afb972e85da9348f03
work_keys_str_mv	AT nigafarov distributionofgeneticmarkersatworkersofaluminumindustrywithprofessionalfluorosisserumproteinsanderythrocyteisoenzymes
_version_	1718417152464125952

Distribution of genetic markers at workers of aluminum industry with professional fluorosis: serum proteins and erythrocyte isoenzymes

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