Biphasic electrical currents stimulation promotes both proliferation and differentiation of fetal neural stem cells.

The use of non-chemical methods to differentiate stem cells has attracted researchers from multiple disciplines, including the engineering and the biomedical fields. No doubt, growth factor based methods are still the most dominant of achieving some level of proliferation and differentiation control...

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Autores principales:	Keun-A Chang, Jin Won Kim, Jeong A Kim, Sung Eun Lee, Saeromi Kim, Won Hyuk Suh, Hye-Sun Kim, Sunghoon Kwon, Sung June Kim, Yoo-Hun Suh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a60e255cd2a74fe6a2ad772b8919a3b3
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spelling	oai:doaj.org-article:a60e255cd2a74fe6a2ad772b8919a3b32021-11-18T06:55:51ZBiphasic electrical currents stimulation promotes both proliferation and differentiation of fetal neural stem cells.1932-620310.1371/journal.pone.0018738https://doaj.org/article/a60e255cd2a74fe6a2ad772b8919a3b32011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21533199/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203The use of non-chemical methods to differentiate stem cells has attracted researchers from multiple disciplines, including the engineering and the biomedical fields. No doubt, growth factor based methods are still the most dominant of achieving some level of proliferation and differentiation control--however, chemical based methods are still limited by the quality, source, and amount of the utilized reagents. Well-defined non-chemical methods to differentiate stem cells allow stem cell scientists to control stem cell biology by precisely administering the pre-defined parameters, whether they are structural cues, substrate stiffness, or in the form of current flow. We have developed a culture system that allows normal stem cell growth and the option of applying continuous and defined levels of electric current to alter the cell biology of growing cells. This biphasic current stimulator chip employing ITO electrodes generates both positive and negative currents in the same culture chamber without affecting surface chemistry. We found that biphasic electrical currents (BECs) significantly increased the proliferation of fetal neural stem cells (NSCs). Furthermore, BECs also promoted the differentiation of fetal NSCs into neuronal cells, as assessed using immunocytochemistry. Our results clearly show that BECs promote both the proliferation and neuronal differentiation of fetal NSCs. It may apply to the development of strategies that employ NSCs in the treatment of various neurodegenerative diseases, such as Alzheimer's and Parkinson's diseases.Keun-A ChangJin Won KimJeong A KimSung Eun LeeSaeromi KimWon Hyuk SuhHye-Sun KimSunghoon KwonSung June KimYoo-Hun SuhPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e18738 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keun-A Chang Jin Won Kim Jeong A Kim Sung Eun Lee Saeromi Kim Won Hyuk Suh Hye-Sun Kim Sunghoon Kwon Sung June Kim Yoo-Hun Suh Biphasic electrical currents stimulation promotes both proliferation and differentiation of fetal neural stem cells.
description	The use of non-chemical methods to differentiate stem cells has attracted researchers from multiple disciplines, including the engineering and the biomedical fields. No doubt, growth factor based methods are still the most dominant of achieving some level of proliferation and differentiation control--however, chemical based methods are still limited by the quality, source, and amount of the utilized reagents. Well-defined non-chemical methods to differentiate stem cells allow stem cell scientists to control stem cell biology by precisely administering the pre-defined parameters, whether they are structural cues, substrate stiffness, or in the form of current flow. We have developed a culture system that allows normal stem cell growth and the option of applying continuous and defined levels of electric current to alter the cell biology of growing cells. This biphasic current stimulator chip employing ITO electrodes generates both positive and negative currents in the same culture chamber without affecting surface chemistry. We found that biphasic electrical currents (BECs) significantly increased the proliferation of fetal neural stem cells (NSCs). Furthermore, BECs also promoted the differentiation of fetal NSCs into neuronal cells, as assessed using immunocytochemistry. Our results clearly show that BECs promote both the proliferation and neuronal differentiation of fetal NSCs. It may apply to the development of strategies that employ NSCs in the treatment of various neurodegenerative diseases, such as Alzheimer's and Parkinson's diseases.
format	article
author	Keun-A Chang Jin Won Kim Jeong A Kim Sung Eun Lee Saeromi Kim Won Hyuk Suh Hye-Sun Kim Sunghoon Kwon Sung June Kim Yoo-Hun Suh
author_facet	Keun-A Chang Jin Won Kim Jeong A Kim Sung Eun Lee Saeromi Kim Won Hyuk Suh Hye-Sun Kim Sunghoon Kwon Sung June Kim Yoo-Hun Suh
author_sort	Keun-A Chang
title	Biphasic electrical currents stimulation promotes both proliferation and differentiation of fetal neural stem cells.
title_short	Biphasic electrical currents stimulation promotes both proliferation and differentiation of fetal neural stem cells.
title_full	Biphasic electrical currents stimulation promotes both proliferation and differentiation of fetal neural stem cells.
title_fullStr	Biphasic electrical currents stimulation promotes both proliferation and differentiation of fetal neural stem cells.
title_full_unstemmed	Biphasic electrical currents stimulation promotes both proliferation and differentiation of fetal neural stem cells.
title_sort	biphasic electrical currents stimulation promotes both proliferation and differentiation of fetal neural stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a60e255cd2a74fe6a2ad772b8919a3b3
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Biphasic electrical currents stimulation promotes both proliferation and differentiation of fetal neural stem cells.

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