A single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales
Abstract In Enterobacterales, the most common carbapenemases are Ambler’s class A (KPC-like), class B (NDM-, VIM- or IMP-like) or class D (OXA-48-like) enzymes. This study describes the characterization of twenty-four OXA-23 or OXA-58 producing-Proteus mirabilis isolates recovered from human and vet...
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Nature Portfolio
2020
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oai:doaj.org-article:a61067770b2c4ff9b0017099f69997912021-12-02T17:52:14ZA single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales10.1038/s41598-020-66161-z2045-2322https://doaj.org/article/a61067770b2c4ff9b0017099f69997912020-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-66161-zhttps://doaj.org/toc/2045-2322Abstract In Enterobacterales, the most common carbapenemases are Ambler’s class A (KPC-like), class B (NDM-, VIM- or IMP-like) or class D (OXA-48-like) enzymes. This study describes the characterization of twenty-four OXA-23 or OXA-58 producing-Proteus mirabilis isolates recovered from human and veterinary samples from France and Belgium. Twenty-two P. mirabilis isolates producing either OXA-23 (n = 21) or OXA-58 (n = 1), collected between 2013 and 2018, as well as 2 reference strains isolated in 1996 and 2015 were fully sequenced. Phylogenetic analysis revealed that 22 of the 24 isolates, including the isolate from 1996, belonged to a single lineage that has disseminated in humans and animals over a long period of time. The bla OXA-23 gene was located on the chromosome and was part of a composite transposon, Tn6703, bracketed by two copies of IS15∆II. Sequencing using Pacbio long read technology of OXA-23-producing P. mirabilis VAC allowed the assembly of a 55.5-kb structure encompassing the bla OXA-23 gene in that isolate. By contrast to the bla OXA-23 genes, the bla OXA-58 gene of P. mirabilis CNR20130297 was identified on a 6-kb plasmid. The acquisition of the bla OXA-58 gene on this plasmid involved XerC-XerD recombinases. Our results suggest that a major clone of OXA-23-producing P. mirabilis is circulating in France and Belgium since 1996.Rémy A. BonninDelphine GirlichAgnès B. JoussetLauraine GauthierGaëlle CuzonPierre BogaertsMarisa HaenniJean-Yves MadecElodie Couvé-DeaconOlivier BarraudNicolas FortineauPhilippe GlaserYouri GlupczynskiLaurent DortetThierry NaasNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-9 (2020) |
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DOAJ |
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EN |
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Medicine R Science Q |
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Medicine R Science Q Rémy A. Bonnin Delphine Girlich Agnès B. Jousset Lauraine Gauthier Gaëlle Cuzon Pierre Bogaerts Marisa Haenni Jean-Yves Madec Elodie Couvé-Deacon Olivier Barraud Nicolas Fortineau Philippe Glaser Youri Glupczynski Laurent Dortet Thierry Naas A single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales |
| description |
Abstract In Enterobacterales, the most common carbapenemases are Ambler’s class A (KPC-like), class B (NDM-, VIM- or IMP-like) or class D (OXA-48-like) enzymes. This study describes the characterization of twenty-four OXA-23 or OXA-58 producing-Proteus mirabilis isolates recovered from human and veterinary samples from France and Belgium. Twenty-two P. mirabilis isolates producing either OXA-23 (n = 21) or OXA-58 (n = 1), collected between 2013 and 2018, as well as 2 reference strains isolated in 1996 and 2015 were fully sequenced. Phylogenetic analysis revealed that 22 of the 24 isolates, including the isolate from 1996, belonged to a single lineage that has disseminated in humans and animals over a long period of time. The bla OXA-23 gene was located on the chromosome and was part of a composite transposon, Tn6703, bracketed by two copies of IS15∆II. Sequencing using Pacbio long read technology of OXA-23-producing P. mirabilis VAC allowed the assembly of a 55.5-kb structure encompassing the bla OXA-23 gene in that isolate. By contrast to the bla OXA-23 genes, the bla OXA-58 gene of P. mirabilis CNR20130297 was identified on a 6-kb plasmid. The acquisition of the bla OXA-58 gene on this plasmid involved XerC-XerD recombinases. Our results suggest that a major clone of OXA-23-producing P. mirabilis is circulating in France and Belgium since 1996. |
| format |
article |
| author |
Rémy A. Bonnin Delphine Girlich Agnès B. Jousset Lauraine Gauthier Gaëlle Cuzon Pierre Bogaerts Marisa Haenni Jean-Yves Madec Elodie Couvé-Deacon Olivier Barraud Nicolas Fortineau Philippe Glaser Youri Glupczynski Laurent Dortet Thierry Naas |
| author_facet |
Rémy A. Bonnin Delphine Girlich Agnès B. Jousset Lauraine Gauthier Gaëlle Cuzon Pierre Bogaerts Marisa Haenni Jean-Yves Madec Elodie Couvé-Deacon Olivier Barraud Nicolas Fortineau Philippe Glaser Youri Glupczynski Laurent Dortet Thierry Naas |
| author_sort |
Rémy A. Bonnin |
| title |
A single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales |
| title_short |
A single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales |
| title_full |
A single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales |
| title_fullStr |
A single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales |
| title_full_unstemmed |
A single Proteus mirabilis lineage from human and animal sources: a hidden reservoir of OXA-23 or OXA-58 carbapenemases in Enterobacterales |
| title_sort |
single proteus mirabilis lineage from human and animal sources: a hidden reservoir of oxa-23 or oxa-58 carbapenemases in enterobacterales |
| publisher |
Nature Portfolio |
| publishDate |
2020 |
| url |
https://doaj.org/article/a61067770b2c4ff9b0017099f6999791 |
| work_keys_str_mv |
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