Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates

Background: Escherichia coli ST131, mainly its H30 clade, is the leading cause of extraintestinal E. coli infections but its correlates of virulence are undefined. Materials and methods: We tested in a murine sepsis model 84 ST131 isolates that differed by country of origin (Spain vs. USA), clonal s...

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Autores principales:	Irene Merino, Stephen B. Porter, Brian Johnston, Connie Clabots, Paul Thuras, Patricia Ruiz-Garbajosa, Rafael Cantón, James R. Johnson
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2020
Materias:	e. coli virulence expec mouse sepsis model Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/a6121412f42142cf8f2a4f9e1c9088a3
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spelling	oai:doaj.org-article:a6121412f42142cf8f2a4f9e1c9088a32021-11-17T14:21:58ZMolecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates2150-55942150-560810.1080/21505594.2020.1747799https://doaj.org/article/a6121412f42142cf8f2a4f9e1c9088a32020-12-01T00:00:00Zhttp://dx.doi.org/10.1080/21505594.2020.1747799https://doaj.org/toc/2150-5594https://doaj.org/toc/2150-5608Background: Escherichia coli ST131, mainly its H30 clade, is the leading cause of extraintestinal E. coli infections but its correlates of virulence are undefined. Materials and methods: We tested in a murine sepsis model 84 ST131 isolates that differed by country of origin (Spain vs. USA), clonal subset, resistance markers, and virulence genes (VGs). Virulence outcomes, including illness severity score (ISS) and “killer” status (>80% mouse lethality), were compared statistically with clonal subset, individual and combined VGs, molecularly defined extraintestinal and uropathogenic E. coli (ExPEC, UPEC) status, and country of origin. Results: Virulence varied widely by strain. Univariable correlates of median ISS and percent “killer” (outcomes if variable present vs. absent) included pap (ISS, 4.4 vs. 3.8; “killer”, 71% vs. 46%), kpsMII (4.1 vs. 2.3; 59% vs. 25%), K2/K100 (4.4 vs. 3.2; 77% vs. 41%), ExPEC (4.2 vs. 2.2; 62% vs. 17%), Spanish origin (4.3 vs. 3.1; 65% vs. 36%), and H30R1 subset (2.5 vs. 4.1; 35% vs. 59%). With multivariable adjustment, ExPEC status was the only consistently significantly predictive variable. Conclusion: Within ST131 the strongest predictor of experimental virulence was molecularly defined ExPEC status. Clonal subsets seemed to behave differently in the murine sepsis model by country of origin.Irene MerinoStephen B. PorterBrian JohnstonConnie ClabotsPaul ThurasPatricia Ruiz-GarbajosaRafael CantónJames R. JohnsonTaylor & Francis Grouparticlee. colivirulenceexpecmouse sepsis modelInfectious and parasitic diseasesRC109-216ENVirulence, Vol 11, Iss 1, Pp 327-336 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	e. coli virulence expec mouse sepsis model Infectious and parasitic diseases RC109-216
spellingShingle	e. coli virulence expec mouse sepsis model Infectious and parasitic diseases RC109-216 Irene Merino Stephen B. Porter Brian Johnston Connie Clabots Paul Thuras Patricia Ruiz-Garbajosa Rafael Cantón James R. Johnson Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates
description	Background: Escherichia coli ST131, mainly its H30 clade, is the leading cause of extraintestinal E. coli infections but its correlates of virulence are undefined. Materials and methods: We tested in a murine sepsis model 84 ST131 isolates that differed by country of origin (Spain vs. USA), clonal subset, resistance markers, and virulence genes (VGs). Virulence outcomes, including illness severity score (ISS) and “killer” status (>80% mouse lethality), were compared statistically with clonal subset, individual and combined VGs, molecularly defined extraintestinal and uropathogenic E. coli (ExPEC, UPEC) status, and country of origin. Results: Virulence varied widely by strain. Univariable correlates of median ISS and percent “killer” (outcomes if variable present vs. absent) included pap (ISS, 4.4 vs. 3.8; “killer”, 71% vs. 46%), kpsMII (4.1 vs. 2.3; 59% vs. 25%), K2/K100 (4.4 vs. 3.2; 77% vs. 41%), ExPEC (4.2 vs. 2.2; 62% vs. 17%), Spanish origin (4.3 vs. 3.1; 65% vs. 36%), and H30R1 subset (2.5 vs. 4.1; 35% vs. 59%). With multivariable adjustment, ExPEC status was the only consistently significantly predictive variable. Conclusion: Within ST131 the strongest predictor of experimental virulence was molecularly defined ExPEC status. Clonal subsets seemed to behave differently in the murine sepsis model by country of origin.
format	article
author	Irene Merino Stephen B. Porter Brian Johnston Connie Clabots Paul Thuras Patricia Ruiz-Garbajosa Rafael Cantón James R. Johnson
author_facet	Irene Merino Stephen B. Porter Brian Johnston Connie Clabots Paul Thuras Patricia Ruiz-Garbajosa Rafael Cantón James R. Johnson
author_sort	Irene Merino
title	Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates
title_short	Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates
title_full	Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates
title_fullStr	Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates
title_full_unstemmed	Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates
title_sort	molecularly defined extraintestinal pathogenic escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among e. coli st131 isolates
publisher	Taylor & Francis Group
publishDate	2020
url	https://doaj.org/article/a6121412f42142cf8f2a4f9e1c9088a3
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Molecularly defined extraintestinal pathogenic Escherichia coli status predicts virulence in a murine sepsis model better than does virotype, individual virulence genes, or clonal subset among E. coli ST131 isolates

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