A unique mitochondrial transcription factor B protein in Dictyostelium discoideum.

Unlike their bacteriophage homologs, mitochondrial RNA polymerases require the assistance of transcription factors in order to transcribe mitochondrial DNA efficiently. The transcription factor A family has been shown to be important for transcription of the human mitochondrial DNA, with some of its...

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Autores principales:	Sam Manna, Phuong Le, Christian Barth
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6153dc990b74e0f90ac0b905e526b67
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spelling	oai:doaj.org-article:a6153dc990b74e0f90ac0b905e526b672021-11-18T09:02:34ZA unique mitochondrial transcription factor B protein in Dictyostelium discoideum.1932-620310.1371/journal.pone.0070614https://doaj.org/article/a6153dc990b74e0f90ac0b905e526b672013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23923009/?tool=EBIhttps://doaj.org/toc/1932-6203Unlike their bacteriophage homologs, mitochondrial RNA polymerases require the assistance of transcription factors in order to transcribe mitochondrial DNA efficiently. The transcription factor A family has been shown to be important for transcription of the human mitochondrial DNA, with some of its regulatory activity located in its extended C-terminal tail. The mitochondrial transcription factor B family often has functions not only in transcription, but also in mitochondrial rRNA modification, a hallmark of its α-proteobacterial origin. We have identified and characterised a mitochondrial transcription factor B homolog in the soil dwelling cellular slime mould Dictyostelium discoideum, an organism widely established as a model for studying eukaryotic cell biology. Using in bacterio functional assays, we demonstrate that the mitochondrial transcription factor B homolog not only functions as a mitochondrial transcription factor, but that it also has a role in rRNA methylation. Additionally, we show that the transcriptional activation properties of the D. discoideum protein are located in its extended C-terminal tail, a feature not seen before in the mitochondrial transcription factor B family, but reminiscent of the human mitochondrial transcription factor A. This report contributes to our current understanding of the complexities of mitochondrial transcription, and its evolution in eukaryotes.Sam MannaPhuong LeChristian BarthPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e70614 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sam Manna Phuong Le Christian Barth A unique mitochondrial transcription factor B protein in Dictyostelium discoideum.
description	Unlike their bacteriophage homologs, mitochondrial RNA polymerases require the assistance of transcription factors in order to transcribe mitochondrial DNA efficiently. The transcription factor A family has been shown to be important for transcription of the human mitochondrial DNA, with some of its regulatory activity located in its extended C-terminal tail. The mitochondrial transcription factor B family often has functions not only in transcription, but also in mitochondrial rRNA modification, a hallmark of its α-proteobacterial origin. We have identified and characterised a mitochondrial transcription factor B homolog in the soil dwelling cellular slime mould Dictyostelium discoideum, an organism widely established as a model for studying eukaryotic cell biology. Using in bacterio functional assays, we demonstrate that the mitochondrial transcription factor B homolog not only functions as a mitochondrial transcription factor, but that it also has a role in rRNA methylation. Additionally, we show that the transcriptional activation properties of the D. discoideum protein are located in its extended C-terminal tail, a feature not seen before in the mitochondrial transcription factor B family, but reminiscent of the human mitochondrial transcription factor A. This report contributes to our current understanding of the complexities of mitochondrial transcription, and its evolution in eukaryotes.
format	article
author	Sam Manna Phuong Le Christian Barth
author_facet	Sam Manna Phuong Le Christian Barth
author_sort	Sam Manna
title	A unique mitochondrial transcription factor B protein in Dictyostelium discoideum.
title_short	A unique mitochondrial transcription factor B protein in Dictyostelium discoideum.
title_full	A unique mitochondrial transcription factor B protein in Dictyostelium discoideum.
title_fullStr	A unique mitochondrial transcription factor B protein in Dictyostelium discoideum.
title_full_unstemmed	A unique mitochondrial transcription factor B protein in Dictyostelium discoideum.
title_sort	unique mitochondrial transcription factor b protein in dictyostelium discoideum.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a6153dc990b74e0f90ac0b905e526b67
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A unique mitochondrial transcription factor B protein in Dictyostelium discoideum.

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