Theme and variations in the evolutionary pathways to virulence of an RNA plant virus species.

The diversity of a highly variable RNA plant virus was considered to determine the range of virulence substitutions, the evolutionary pathways to virulence, and whether intraspecific diversity modulates virulence pathways and propensity. In all, 114 isolates representative of the genetic and geograp...

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Autores principales:	Agnès Pinel-Galzi, Mbolarinosy Rakotomalala, Emmanuel Sangu, Fatogoma Sorho, Zakaria Kanyeka, Oumar Traoré, Drissa Sérémé, Nils Poulicard, Yvonne Rabenantoandro, Yacouba Séré, Gnissa Konaté, Alain Ghesquière, Eugénie Hébrard, Denis Fargette
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2007
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a615618b5c204367bf79a343371f4d90
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spelling	oai:doaj.org-article:a615618b5c204367bf79a343371f4d902021-11-25T05:46:49ZTheme and variations in the evolutionary pathways to virulence of an RNA plant virus species.1553-73661553-737410.1371/journal.ppat.0030180https://doaj.org/article/a615618b5c204367bf79a343371f4d902007-11-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.0030180https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The diversity of a highly variable RNA plant virus was considered to determine the range of virulence substitutions, the evolutionary pathways to virulence, and whether intraspecific diversity modulates virulence pathways and propensity. In all, 114 isolates representative of the genetic and geographic diversity of Rice yellow mottle virus (RYMV) in Africa were inoculated to several cultivars with eIF(iso)4G-mediated Rymv1-2 resistance. Altogether, 41 virulent variants generated from ten wild isolates were analyzed. Nonconservative amino acid replacements at five positions located within a stretch of 15 codons in the central region of the 79-aa-long protein VPg were associated with virulence. Virulence substitutions were fixed predominantly at codon 48 in most strains, whatever the host genetic background or the experimental conditions. There were one major and two isolate-specific mutational pathways conferring virulence at codon 48. In the prevalent mutational pathway I, arginine (AGA) was successively displaced by glycine (GGA) and glutamic acid (GAA). Substitutions in the other virulence codons were displaced when E48 was fixed. In the isolate-specific mutational pathway II, isoleucine (ATA) emerged and often later coexisted with valine (GTA). In mutational pathway III, arginine, with the specific S2/S3 strain codon usage AGG, was displaced by tryptophane (TGG). Mutational pathway I never arose in the widely spread West African S2/S3 strain because G48 was not infectious in the S2/S3 genetic context. Strain S2/S3 least frequently overcame resistance, whereas two geographically localized variants of the strain S4 had a high propensity to virulence. Codons 49 and 26 of the VPg, under diversifying selection, are candidate positions in modulating the genetic barriers to virulence. The theme and variations in the evolutionary pathways to virulence of RYMV illustrates the extent of parallel evolution within a highly variable RNA plant virus species.Agnès Pinel-GalziMbolarinosy RakotomalalaEmmanuel SanguFatogoma SorhoZakaria KanyekaOumar TraoréDrissa SéréméNils PoulicardYvonne RabenantoandroYacouba SéréGnissa KonatéAlain GhesquièreEugénie HébrardDenis FargettePublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 3, Iss 11, p e180 (2007)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Agnès Pinel-Galzi Mbolarinosy Rakotomalala Emmanuel Sangu Fatogoma Sorho Zakaria Kanyeka Oumar Traoré Drissa Sérémé Nils Poulicard Yvonne Rabenantoandro Yacouba Séré Gnissa Konaté Alain Ghesquière Eugénie Hébrard Denis Fargette Theme and variations in the evolutionary pathways to virulence of an RNA plant virus species.
description	The diversity of a highly variable RNA plant virus was considered to determine the range of virulence substitutions, the evolutionary pathways to virulence, and whether intraspecific diversity modulates virulence pathways and propensity. In all, 114 isolates representative of the genetic and geographic diversity of Rice yellow mottle virus (RYMV) in Africa were inoculated to several cultivars with eIF(iso)4G-mediated Rymv1-2 resistance. Altogether, 41 virulent variants generated from ten wild isolates were analyzed. Nonconservative amino acid replacements at five positions located within a stretch of 15 codons in the central region of the 79-aa-long protein VPg were associated with virulence. Virulence substitutions were fixed predominantly at codon 48 in most strains, whatever the host genetic background or the experimental conditions. There were one major and two isolate-specific mutational pathways conferring virulence at codon 48. In the prevalent mutational pathway I, arginine (AGA) was successively displaced by glycine (GGA) and glutamic acid (GAA). Substitutions in the other virulence codons were displaced when E48 was fixed. In the isolate-specific mutational pathway II, isoleucine (ATA) emerged and often later coexisted with valine (GTA). In mutational pathway III, arginine, with the specific S2/S3 strain codon usage AGG, was displaced by tryptophane (TGG). Mutational pathway I never arose in the widely spread West African S2/S3 strain because G48 was not infectious in the S2/S3 genetic context. Strain S2/S3 least frequently overcame resistance, whereas two geographically localized variants of the strain S4 had a high propensity to virulence. Codons 49 and 26 of the VPg, under diversifying selection, are candidate positions in modulating the genetic barriers to virulence. The theme and variations in the evolutionary pathways to virulence of RYMV illustrates the extent of parallel evolution within a highly variable RNA plant virus species.
format	article
author	Agnès Pinel-Galzi Mbolarinosy Rakotomalala Emmanuel Sangu Fatogoma Sorho Zakaria Kanyeka Oumar Traoré Drissa Sérémé Nils Poulicard Yvonne Rabenantoandro Yacouba Séré Gnissa Konaté Alain Ghesquière Eugénie Hébrard Denis Fargette
author_facet	Agnès Pinel-Galzi Mbolarinosy Rakotomalala Emmanuel Sangu Fatogoma Sorho Zakaria Kanyeka Oumar Traoré Drissa Sérémé Nils Poulicard Yvonne Rabenantoandro Yacouba Séré Gnissa Konaté Alain Ghesquière Eugénie Hébrard Denis Fargette
author_sort	Agnès Pinel-Galzi
title	Theme and variations in the evolutionary pathways to virulence of an RNA plant virus species.
title_short	Theme and variations in the evolutionary pathways to virulence of an RNA plant virus species.
title_full	Theme and variations in the evolutionary pathways to virulence of an RNA plant virus species.
title_fullStr	Theme and variations in the evolutionary pathways to virulence of an RNA plant virus species.
title_full_unstemmed	Theme and variations in the evolutionary pathways to virulence of an RNA plant virus species.
title_sort	theme and variations in the evolutionary pathways to virulence of an rna plant virus species.
publisher	Public Library of Science (PLoS)
publishDate	2007
url	https://doaj.org/article/a615618b5c204367bf79a343371f4d90
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Theme and variations in the evolutionary pathways to virulence of an RNA plant virus species.

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