The Escherichia coli SOS gene dinF protects against oxidative stress and bile salts.

DNA is constantly damaged by physical and chemical factors, including reactive oxygen species (ROS), such as superoxide radical (O(2)(-)), hydrogen peroxide (H(2)O(2)) and hydroxyl radical (•OH). Specific mechanisms to protect and repair DNA lesions produced by ROS have been developed in living bein...

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Autores principales:	Jerónimo Rodríguez-Beltrán, Alexandro Rodríguez-Rojas, Javier R Guelfo, Alejandro Couce, Jesús Blázquez
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6192928393b4a8caf02702ee2118604
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spelling	oai:doaj.org-article:a6192928393b4a8caf02702ee21186042021-11-18T07:22:05ZThe Escherichia coli SOS gene dinF protects against oxidative stress and bile salts.1932-620310.1371/journal.pone.0034791https://doaj.org/article/a6192928393b4a8caf02702ee21186042012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22523558/?tool=EBIhttps://doaj.org/toc/1932-6203DNA is constantly damaged by physical and chemical factors, including reactive oxygen species (ROS), such as superoxide radical (O(2)(-)), hydrogen peroxide (H(2)O(2)) and hydroxyl radical (•OH). Specific mechanisms to protect and repair DNA lesions produced by ROS have been developed in living beings. In Escherichia coli the SOS system, an inducible response activated to rescue cells from severe DNA damage, is a network that regulates the expression of more than 40 genes in response to this damage, many of them playing important roles in DNA damage tolerance mechanisms. Although the function of most of these genes has been elucidated, the activity of some others, such as dinF, remains unknown. The DinF deduced polypeptide sequence shows a high homology with membrane proteins of the multidrug and toxic compound extrusion (MATE) family. We describe here that expression of dinF protects against bile salts, probably by decreasing the effects of ROS, which is consistent with the observed decrease in H(2)O(2)-killing and protein carbonylation. These results, together with its ability to decrease the level of intracellular ROS, suggests that DinF can detoxify, either direct or indirectly, oxidizing molecules that can damage DNA and proteins from both the bacterial metabolism and the environment. Although the exact mechanism of DinF activity remains to be identified, we describe for the first time a role for dinF.Jerónimo Rodríguez-BeltránAlexandro Rodríguez-RojasJavier R GuelfoAlejandro CouceJesús BlázquezPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e34791 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jerónimo Rodríguez-Beltrán Alexandro Rodríguez-Rojas Javier R Guelfo Alejandro Couce Jesús Blázquez The Escherichia coli SOS gene dinF protects against oxidative stress and bile salts.
description	DNA is constantly damaged by physical and chemical factors, including reactive oxygen species (ROS), such as superoxide radical (O(2)(-)), hydrogen peroxide (H(2)O(2)) and hydroxyl radical (•OH). Specific mechanisms to protect and repair DNA lesions produced by ROS have been developed in living beings. In Escherichia coli the SOS system, an inducible response activated to rescue cells from severe DNA damage, is a network that regulates the expression of more than 40 genes in response to this damage, many of them playing important roles in DNA damage tolerance mechanisms. Although the function of most of these genes has been elucidated, the activity of some others, such as dinF, remains unknown. The DinF deduced polypeptide sequence shows a high homology with membrane proteins of the multidrug and toxic compound extrusion (MATE) family. We describe here that expression of dinF protects against bile salts, probably by decreasing the effects of ROS, which is consistent with the observed decrease in H(2)O(2)-killing and protein carbonylation. These results, together with its ability to decrease the level of intracellular ROS, suggests that DinF can detoxify, either direct or indirectly, oxidizing molecules that can damage DNA and proteins from both the bacterial metabolism and the environment. Although the exact mechanism of DinF activity remains to be identified, we describe for the first time a role for dinF.
format	article
author	Jerónimo Rodríguez-Beltrán Alexandro Rodríguez-Rojas Javier R Guelfo Alejandro Couce Jesús Blázquez
author_facet	Jerónimo Rodríguez-Beltrán Alexandro Rodríguez-Rojas Javier R Guelfo Alejandro Couce Jesús Blázquez
author_sort	Jerónimo Rodríguez-Beltrán
title	The Escherichia coli SOS gene dinF protects against oxidative stress and bile salts.
title_short	The Escherichia coli SOS gene dinF protects against oxidative stress and bile salts.
title_full	The Escherichia coli SOS gene dinF protects against oxidative stress and bile salts.
title_fullStr	The Escherichia coli SOS gene dinF protects against oxidative stress and bile salts.
title_full_unstemmed	The Escherichia coli SOS gene dinF protects against oxidative stress and bile salts.
title_sort	escherichia coli sos gene dinf protects against oxidative stress and bile salts.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a6192928393b4a8caf02702ee2118604
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The Escherichia coli SOS gene dinF protects against oxidative stress and bile salts.

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