CSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens

Off-target effects often confound the interpretation of CRISPR screens. Here, the authors introduce a computational method that corrects for off-targets in gRNA depletion assays, circumventing the need to completely discard unspecific guides.

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Autores principales:	Alexendar R. Perez, Laura Sala, Richard K. Perez, Joana A. Vidigal
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/a61d9a2685ec4a239acd6ead2d346875
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spelling	oai:doaj.org-article:a61d9a2685ec4a239acd6ead2d3468752021-11-14T12:35:46ZCSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens10.1038/s41467-021-26722-w2041-1723https://doaj.org/article/a61d9a2685ec4a239acd6ead2d3468752021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26722-whttps://doaj.org/toc/2041-1723Off-target effects often confound the interpretation of CRISPR screens. Here, the authors introduce a computational method that corrects for off-targets in gRNA depletion assays, circumventing the need to completely discard unspecific guides.Alexendar R. PerezLaura SalaRichard K. PerezJoana A. VidigalNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Alexendar R. Perez Laura Sala Richard K. Perez Joana A. Vidigal CSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens
description	Off-target effects often confound the interpretation of CRISPR screens. Here, the authors introduce a computational method that corrects for off-targets in gRNA depletion assays, circumventing the need to completely discard unspecific guides.
format	article
author	Alexendar R. Perez Laura Sala Richard K. Perez Joana A. Vidigal
author_facet	Alexendar R. Perez Laura Sala Richard K. Perez Joana A. Vidigal
author_sort	Alexendar R. Perez
title	CSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens
title_short	CSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens
title_full	CSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens
title_fullStr	CSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens
title_full_unstemmed	CSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens
title_sort	csc software corrects off-target mediated grna depletion in crispr-cas9 essentiality screens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a61d9a2685ec4a239acd6ead2d346875
work_keys_str_mv	AT alexendarrperez cscsoftwarecorrectsofftargetmediatedgrnadepletionincrisprcas9essentialityscreens AT laurasala cscsoftwarecorrectsofftargetmediatedgrnadepletionincrisprcas9essentialityscreens AT richardkperez cscsoftwarecorrectsofftargetmediatedgrnadepletionincrisprcas9essentialityscreens AT joanaavidigal cscsoftwarecorrectsofftargetmediatedgrnadepletionincrisprcas9essentialityscreens
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CSC software corrects off-target mediated gRNA depletion in CRISPR-Cas9 essentiality screens

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