Rapid and efficient immunomagnetic isolation of endothelial cells from human peripheral nerves

Abstract Endothelial cells (ECs) have gained an increased scientific focus since they were reported to provide guidance for Schwann cells and subsequently following axons after nerve injuries. However, previous protocols for the isolation of nerve-derived ECs from human nerves are ineffective regard...

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Autores principales:	Patrick Dömer, Janine Kayal, Ulrike Janssen-Bienhold, Bettina Kewitz, Thomas Kretschmer, Christian Heinen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a62feb788c984593bc77309f18d78491
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spelling	oai:doaj.org-article:a62feb788c984593bc77309f18d784912021-12-02T10:49:11ZRapid and efficient immunomagnetic isolation of endothelial cells from human peripheral nerves10.1038/s41598-021-81361-x2045-2322https://doaj.org/article/a62feb788c984593bc77309f18d784912021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81361-xhttps://doaj.org/toc/2045-2322Abstract Endothelial cells (ECs) have gained an increased scientific focus since they were reported to provide guidance for Schwann cells and subsequently following axons after nerve injuries. However, previous protocols for the isolation of nerve-derived ECs from human nerves are ineffective regarding time and yield. Therefore, we established a novel and efficient protocol for the isolation of ECs from human peripheral nerves by means of immunomagnetic CD31-antibody conjugated Dynabeads and assessed the purity of the isolated cells. The easy-to-follow and time-effective isolation method allows the isolation of > 95% pure ECs. The isolated ECs were shown to express highly specific EC marker proteins and revealed functional properties by formation of CD31 and VE-cadherin positive adherens junctions, as well as ZO-1 positive tight-junctions. Moreover, the formation of capillary EC-tubes was observed in-vitro. The novel protocol for the isolation of human nerve-derived ECs allows and simplifies the usage of ECs in research of the human blood-nerve-barrier and peripheral nerve regeneration. Additionally, a potential experimental application of patient-derived nerve ECs in the in-vitro vascularization of artificial nerve grafts is feasible.Patrick DömerJanine KayalUlrike Janssen-BienholdBettina KewitzThomas KretschmerChristian HeinenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patrick Dömer Janine Kayal Ulrike Janssen-Bienhold Bettina Kewitz Thomas Kretschmer Christian Heinen Rapid and efficient immunomagnetic isolation of endothelial cells from human peripheral nerves
description	Abstract Endothelial cells (ECs) have gained an increased scientific focus since they were reported to provide guidance for Schwann cells and subsequently following axons after nerve injuries. However, previous protocols for the isolation of nerve-derived ECs from human nerves are ineffective regarding time and yield. Therefore, we established a novel and efficient protocol for the isolation of ECs from human peripheral nerves by means of immunomagnetic CD31-antibody conjugated Dynabeads and assessed the purity of the isolated cells. The easy-to-follow and time-effective isolation method allows the isolation of > 95% pure ECs. The isolated ECs were shown to express highly specific EC marker proteins and revealed functional properties by formation of CD31 and VE-cadherin positive adherens junctions, as well as ZO-1 positive tight-junctions. Moreover, the formation of capillary EC-tubes was observed in-vitro. The novel protocol for the isolation of human nerve-derived ECs allows and simplifies the usage of ECs in research of the human blood-nerve-barrier and peripheral nerve regeneration. Additionally, a potential experimental application of patient-derived nerve ECs in the in-vitro vascularization of artificial nerve grafts is feasible.
format	article
author	Patrick Dömer Janine Kayal Ulrike Janssen-Bienhold Bettina Kewitz Thomas Kretschmer Christian Heinen
author_facet	Patrick Dömer Janine Kayal Ulrike Janssen-Bienhold Bettina Kewitz Thomas Kretschmer Christian Heinen
author_sort	Patrick Dömer
title	Rapid and efficient immunomagnetic isolation of endothelial cells from human peripheral nerves
title_short	Rapid and efficient immunomagnetic isolation of endothelial cells from human peripheral nerves
title_full	Rapid and efficient immunomagnetic isolation of endothelial cells from human peripheral nerves
title_fullStr	Rapid and efficient immunomagnetic isolation of endothelial cells from human peripheral nerves
title_full_unstemmed	Rapid and efficient immunomagnetic isolation of endothelial cells from human peripheral nerves
title_sort	rapid and efficient immunomagnetic isolation of endothelial cells from human peripheral nerves
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a62feb788c984593bc77309f18d78491
work_keys_str_mv	AT patrickdomer rapidandefficientimmunomagneticisolationofendothelialcellsfromhumanperipheralnerves AT janinekayal rapidandefficientimmunomagneticisolationofendothelialcellsfromhumanperipheralnerves AT ulrikejanssenbienhold rapidandefficientimmunomagneticisolationofendothelialcellsfromhumanperipheralnerves AT bettinakewitz rapidandefficientimmunomagneticisolationofendothelialcellsfromhumanperipheralnerves AT thomaskretschmer rapidandefficientimmunomagneticisolationofendothelialcellsfromhumanperipheralnerves AT christianheinen rapidandefficientimmunomagneticisolationofendothelialcellsfromhumanperipheralnerves
_version_	1718396610138865664

Rapid and efficient immunomagnetic isolation of endothelial cells from human peripheral nerves

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