Amplify antimicrobial photo dynamic therapy efficacy with poly-beta-amino esters (PBAEs)

Abstract Light-activated antimicrobial agents (photosensitisers) are promising alternatives to antibiotics for the treatment of skin infections and wounds through antimicrobial photo dynamic therapy (aPDT); utilisation of this technique is still restricted by general low efficacy requiring long expo...

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Autores principales:	Stefano Perni, Emily C. Preedy, Polina Prokopovich
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6330b1258d84117929ccf7e61040faa
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spelling	oai:doaj.org-article:a6330b1258d84117929ccf7e61040faa2021-12-02T18:17:42ZAmplify antimicrobial photo dynamic therapy efficacy with poly-beta-amino esters (PBAEs)10.1038/s41598-021-86773-32045-2322https://doaj.org/article/a6330b1258d84117929ccf7e61040faa2021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86773-3https://doaj.org/toc/2045-2322Abstract Light-activated antimicrobial agents (photosensitisers) are promising alternatives to antibiotics for the treatment of skin infections and wounds through antimicrobial photo dynamic therapy (aPDT); utilisation of this technique is still restricted by general low efficacy requiring long exposure time (in the order of tens of minutes) that make the treatment very resource intensive. We report for the first time the possibility of harvesting the cell penetrating properties of poly-beta-amino esters (PBAEs) in combination with toluidine blue O (TBO) to shorten aPDT exposure time. Candidates capable of inactivation rates 30 times quicker than pure TBO were discovered and further improvements through PBAE backbone optimisation could be foreseen. Efficacy of the complexes was PBAE-dependent on a combination of TBO uptake and a newly discovered and unexpected role of PBAEs on reactive species production. Chemometric approach of partial least square regression was employed to assess the critical PBAE properties involved in this newly observed phenomenon in order to elicit a possible mechanism. The superior antimicrobial performance of this new approach benefits from the use of well established, low-cost and safe dye (TBO) coupled with inexpensive, widely tested and biodegradable polymers also known to be safe. Moreover, no adverse cytotoxic effects of the PBAEs adjuvated TBO delivery have been observed on a skin cells in vitro model demonstrating the safety profile of this new technology.Stefano PerniEmily C. PreedyPolina ProkopovichNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefano Perni Emily C. Preedy Polina Prokopovich Amplify antimicrobial photo dynamic therapy efficacy with poly-beta-amino esters (PBAEs)
description	Abstract Light-activated antimicrobial agents (photosensitisers) are promising alternatives to antibiotics for the treatment of skin infections and wounds through antimicrobial photo dynamic therapy (aPDT); utilisation of this technique is still restricted by general low efficacy requiring long exposure time (in the order of tens of minutes) that make the treatment very resource intensive. We report for the first time the possibility of harvesting the cell penetrating properties of poly-beta-amino esters (PBAEs) in combination with toluidine blue O (TBO) to shorten aPDT exposure time. Candidates capable of inactivation rates 30 times quicker than pure TBO were discovered and further improvements through PBAE backbone optimisation could be foreseen. Efficacy of the complexes was PBAE-dependent on a combination of TBO uptake and a newly discovered and unexpected role of PBAEs on reactive species production. Chemometric approach of partial least square regression was employed to assess the critical PBAE properties involved in this newly observed phenomenon in order to elicit a possible mechanism. The superior antimicrobial performance of this new approach benefits from the use of well established, low-cost and safe dye (TBO) coupled with inexpensive, widely tested and biodegradable polymers also known to be safe. Moreover, no adverse cytotoxic effects of the PBAEs adjuvated TBO delivery have been observed on a skin cells in vitro model demonstrating the safety profile of this new technology.
format	article
author	Stefano Perni Emily C. Preedy Polina Prokopovich
author_facet	Stefano Perni Emily C. Preedy Polina Prokopovich
author_sort	Stefano Perni
title	Amplify antimicrobial photo dynamic therapy efficacy with poly-beta-amino esters (PBAEs)
title_short	Amplify antimicrobial photo dynamic therapy efficacy with poly-beta-amino esters (PBAEs)
title_full	Amplify antimicrobial photo dynamic therapy efficacy with poly-beta-amino esters (PBAEs)
title_fullStr	Amplify antimicrobial photo dynamic therapy efficacy with poly-beta-amino esters (PBAEs)
title_full_unstemmed	Amplify antimicrobial photo dynamic therapy efficacy with poly-beta-amino esters (PBAEs)
title_sort	amplify antimicrobial photo dynamic therapy efficacy with poly-beta-amino esters (pbaes)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a6330b1258d84117929ccf7e61040faa
work_keys_str_mv	AT stefanoperni amplifyantimicrobialphotodynamictherapyefficacywithpolybetaaminoesterspbaes AT emilycpreedy amplifyantimicrobialphotodynamictherapyefficacywithpolybetaaminoesterspbaes AT polinaprokopovich amplifyantimicrobialphotodynamictherapyefficacywithpolybetaaminoesterspbaes
_version_	1718378291644071936

Amplify antimicrobial photo dynamic therapy efficacy with poly-beta-amino esters (PBAEs)

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