Cell-by-cell alignment of repeated specular microscopy images from the same eye.

<h4>Purpose</h4>Modern specular microscopes (SM) robustly depict the same central area of the corneal endothelium at different time points through a built-in fixation light. However, repeated image acquisitions slightly shift and rotate because of minute changes in head position in the c...

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Autores principales:	Daniel Böhringer, Stefan Lang, Thomas Reinhard
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a63f661183f6406c8ca6e2e3ff7edc3f
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spelling	oai:doaj.org-article:a63f661183f6406c8ca6e2e3ff7edc3f2021-11-18T07:53:22ZCell-by-cell alignment of repeated specular microscopy images from the same eye.1932-620310.1371/journal.pone.0059261https://doaj.org/article/a63f661183f6406c8ca6e2e3ff7edc3f2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23516618/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Purpose</h4>Modern specular microscopes (SM) robustly depict the same central area of the corneal endothelium at different time points through a built-in fixation light. However, repeated image acquisitions slightly shift and rotate because of minute changes in head position in the chin and forehead rest. This prevents the manual retrieval of individual corneal endothelial cells (CECs) in repeated measurements because SM images usually lack obvious landmarks. We devised and validated an image registration algorithm that aligns SM images from the same eye to make corresponding CECs coincide.<h4>Methods</h4>We retrospectively selected 27 image pairs for the presence of significant image overlap. Each image pair had been recorded on the same day and of the same eye. We applied our registration method in each image pair. Two observers independently validated, by means of alternation flicker, that the image pairs had been correctly aligned. We also repeatedly applied our registration method on unrelated image pairs by randomly drawing images and making certain that the images did not originate from the same eye. This was done to assess the specifity of our method.<h4>Results</h4>All automated registrations of the same-day and same-eye image pairs were accurate. However, one single image incorrectly failed to trigger the non-match diagnosis twice in 81 registration attempts between unrelated images. As it turned out, this particular image depicted only 73 CECs. The average number of CECs was 253 (range 73-393).<h4>Conclusion</h4>Repeated non-contact SM images can be automatedly aligned so that the corresponding CECs coincide. Any successful alignment can be considered as proof of the retrieval of identical CECs as soon as at least 100 CEC centroids have been identified. We believe our method is the first to robustly confirm endothelial stability in individual eyes.Daniel BöhringerStefan LangThomas ReinhardPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 3, p e59261 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Böhringer Stefan Lang Thomas Reinhard Cell-by-cell alignment of repeated specular microscopy images from the same eye.
description	<h4>Purpose</h4>Modern specular microscopes (SM) robustly depict the same central area of the corneal endothelium at different time points through a built-in fixation light. However, repeated image acquisitions slightly shift and rotate because of minute changes in head position in the chin and forehead rest. This prevents the manual retrieval of individual corneal endothelial cells (CECs) in repeated measurements because SM images usually lack obvious landmarks. We devised and validated an image registration algorithm that aligns SM images from the same eye to make corresponding CECs coincide.<h4>Methods</h4>We retrospectively selected 27 image pairs for the presence of significant image overlap. Each image pair had been recorded on the same day and of the same eye. We applied our registration method in each image pair. Two observers independently validated, by means of alternation flicker, that the image pairs had been correctly aligned. We also repeatedly applied our registration method on unrelated image pairs by randomly drawing images and making certain that the images did not originate from the same eye. This was done to assess the specifity of our method.<h4>Results</h4>All automated registrations of the same-day and same-eye image pairs were accurate. However, one single image incorrectly failed to trigger the non-match diagnosis twice in 81 registration attempts between unrelated images. As it turned out, this particular image depicted only 73 CECs. The average number of CECs was 253 (range 73-393).<h4>Conclusion</h4>Repeated non-contact SM images can be automatedly aligned so that the corresponding CECs coincide. Any successful alignment can be considered as proof of the retrieval of identical CECs as soon as at least 100 CEC centroids have been identified. We believe our method is the first to robustly confirm endothelial stability in individual eyes.
format	article
author	Daniel Böhringer Stefan Lang Thomas Reinhard
author_facet	Daniel Böhringer Stefan Lang Thomas Reinhard
author_sort	Daniel Böhringer
title	Cell-by-cell alignment of repeated specular microscopy images from the same eye.
title_short	Cell-by-cell alignment of repeated specular microscopy images from the same eye.
title_full	Cell-by-cell alignment of repeated specular microscopy images from the same eye.
title_fullStr	Cell-by-cell alignment of repeated specular microscopy images from the same eye.
title_full_unstemmed	Cell-by-cell alignment of repeated specular microscopy images from the same eye.
title_sort	cell-by-cell alignment of repeated specular microscopy images from the same eye.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a63f661183f6406c8ca6e2e3ff7edc3f
work_keys_str_mv	AT danielbohringer cellbycellalignmentofrepeatedspecularmicroscopyimagesfromthesameeye AT stefanlang cellbycellalignmentofrepeatedspecularmicroscopyimagesfromthesameeye AT thomasreinhard cellbycellalignmentofrepeatedspecularmicroscopyimagesfromthesameeye
_version_	1718422820244946944

Cell-by-cell alignment of repeated specular microscopy images from the same eye.

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