Empagliflozin Protects Against Proximal Renal Tubular Cell Injury Induced by High Glucose via Regulation of Hypoxia-Inducible Factor 1-Alpha
Angelamellisy Revelian Ndibalema,1,2 Deo Kabuye,3,4 Si Wen,1 Lulu Li,1 Xin Li,1 Qiuling Fan1 1Department of Nephrology, The First Hospital of China Medical University, Shenyang 110001, People’s Republic of China; 2Kairuki Hospital, Dar es Salaam, Tanzania; 3Department of Laboratory Medicin...
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| Formato: | article |
| Lenguaje: | EN |
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Dove Medical Press
2020
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| Acceso en línea: | https://doaj.org/article/a643b230f7d6409eb67c42e9d3aa3e48 |
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| Sumario: | Angelamellisy Revelian Ndibalema,1,2 Deo Kabuye,3,4 Si Wen,1 Lulu Li,1 Xin Li,1 Qiuling Fan1 1Department of Nephrology, The First Hospital of China Medical University, Shenyang 110001, People’s Republic of China; 2Kairuki Hospital, Dar es Salaam, Tanzania; 3Department of Laboratory Medicine, The First Affiliated Hospital of China Medical University, Shenyang, 110001, People’s Republic of China; 4Kalisizo Hospital Uganda, Kalisizo, UgandaCorrespondence: Qiuling Fan Tel +86 13904012680Email cmufql@163.comBackground: Evidence from both animal and human studies clearly supports the renal beneficial effects of empagliflozin (emp), a sodium glucose co-transporter 2 (SGLT2) inhibitor, but the mechanism in which it exerts its effect is not well understood. In this study, we investigated the capability of emp on reducing hyperglycemia-induced renal proximal tubular epithelial cells injury and we evaluated if the renoprotective effect of emp associates with hypoxia-inducible factor-1α (HIF-1α).Materials and Methods: Human kidney cell lines (HK-2 cells) were incubated in normoxia, high glucose with or without emp treatment for 72 hours to evaluate the induction of HIF-1α, glucose transporter-1, SGLT2, the fibrosis signal pathway and epithelial–mesenchymal transition (EMT) markers.Results: High glucose (HG) increased expression of Collagen IV, Fibronectin, transforming growth factor-beta1 (TGF-β 1). However, emp treatment remarkably decreased expression of TGF-β 1, accumulation of extracellular matrix proteins (Fibronectin, Collagen IV), as well as (phosphorylated-smad3) P-smad3. HG increased SGLT2 protein expression compared to normal glucose (NG) while emp significantly decreased SGLT2 expression. Furthermore, emp decreased high glucose-induced alpha-smooth muscle actin (α-SMA) expression and reversed epithelial marker (E-catherin) suppression induced by high glucose. In addition, emp treatment for 72 h increased expression of HIF-1α protein (95% CI: -0.5918 to – 0.002338, at 100nM, P < 0.05, 95% CI – 0.6631 to – 0.07367 at 500nM, P < 0.05) in hyperglycemic normoxic HK-2 cells. Furthermore, we observed increased expression of GLUT-1 protein after emp treatment and remarkably decreased cell proliferation.Conclusion: Emp treatment protected proximal renal tubular cells injury induced by high glucose. Induction of HIF-1α expression by emp may play an essential role in the protection of high glucose-induced proximal renal tubular epithelial cells injury.Keywords: humans, animals, transforming growth factor-beta1, empagliflozin, diabetic nephropathies, glucose |
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