Myc promoter-binding protein-1 (MBP-1) is a novel potential prognostic marker in invasive ductal breast carcinoma.

<h4>Background</h4>Alpha-enolase is a glycolytic enzyme that catalyses the formation of phosphoenolpyruvate in the cell cytoplasm. α-Enolase and the predominantly nuclear Myc promoter-binding protein-1 (MBP-1) originate from a single gene through the alternative use of translational star...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Mariavera Lo Presti, Arianna Ferro, Flavia Contino, Claudia Mazzarella, Silvia Sbacchi, Elena Roz, Carmelo Lupo, Giovanni Perconti, Agata Giallongo, Paola Migliorini, Antonio Marrazzo, Salvatore Feo
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2010
Materias:
R
Q
Acceso en línea:https://doaj.org/article/a6468528a5b34ea8ac7e8ee73efc9933
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:a6468528a5b34ea8ac7e8ee73efc9933
record_format dspace
spelling oai:doaj.org-article:a6468528a5b34ea8ac7e8ee73efc99332021-11-18T06:34:53ZMyc promoter-binding protein-1 (MBP-1) is a novel potential prognostic marker in invasive ductal breast carcinoma.1932-620310.1371/journal.pone.0012961https://doaj.org/article/a6468528a5b34ea8ac7e8ee73efc99332010-09-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20886042/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Alpha-enolase is a glycolytic enzyme that catalyses the formation of phosphoenolpyruvate in the cell cytoplasm. α-Enolase and the predominantly nuclear Myc promoter-binding protein-1 (MBP-1) originate from a single gene through the alternative use of translational starting sites. MBP-1 binds to the P2 c-myc promoter and competes with TATA-box binding protein (TBP) to suppress gene transcription. Although several studies have shown an antiproliferative effect of MBP-1 overexpression on several human cancer cells, to date detailed observations of α-enolase and MBP-1 relative expression in primary tumors versus normal tissues and their correlation with clinicopathological features have not been undertaken.<h4>Methodology and findings</h4>We analyzed α-enolase and MBP-1 expression in normal breast epithelium and primary invasive ductal breast carcinoma (IDC) from 177 patients by Western blot and immunohistochemical analyses, using highly specific anti-α-enolase monoclonal antibodies. A significant increase in the expression of cytoplasmic α-enolase was observed in 98% of the tumors analysed, compared to normal tissues. Nuclear MBP-1 was found in almost all the normal tissues while its expression was retained in only 35% of the tumors. Statistically significant associations were observed among the nuclear expression of MBP-1 and ErbB2 status, Ki-67 expression, node status and tumor grade. Furthermore MBP-1 expression was associated with good survival of patients with IDC.<h4>Conclusions</h4>MBP-1 functions in repressing c-myc gene expression and the results presented indicate that the loss of nuclear MBP-1 expression in a large number of IDC may be a critical step in the development and progression of breast cancer and a predictor of adverse outcome. Nuclear MBP-1 appears to be a novel and valuable histochemical marker with potential prognostic value in breast cancer.Mariavera Lo PrestiArianna FerroFlavia ContinoClaudia MazzarellaSilvia SbacchiElena RozCarmelo LupoGiovanni PercontiAgata GiallongoPaola MiglioriniAntonio MarrazzoSalvatore FeoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 9, p e12961 (2010)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Mariavera Lo Presti
Arianna Ferro
Flavia Contino
Claudia Mazzarella
Silvia Sbacchi
Elena Roz
Carmelo Lupo
Giovanni Perconti
Agata Giallongo
Paola Migliorini
Antonio Marrazzo
Salvatore Feo
Myc promoter-binding protein-1 (MBP-1) is a novel potential prognostic marker in invasive ductal breast carcinoma.
description <h4>Background</h4>Alpha-enolase is a glycolytic enzyme that catalyses the formation of phosphoenolpyruvate in the cell cytoplasm. α-Enolase and the predominantly nuclear Myc promoter-binding protein-1 (MBP-1) originate from a single gene through the alternative use of translational starting sites. MBP-1 binds to the P2 c-myc promoter and competes with TATA-box binding protein (TBP) to suppress gene transcription. Although several studies have shown an antiproliferative effect of MBP-1 overexpression on several human cancer cells, to date detailed observations of α-enolase and MBP-1 relative expression in primary tumors versus normal tissues and their correlation with clinicopathological features have not been undertaken.<h4>Methodology and findings</h4>We analyzed α-enolase and MBP-1 expression in normal breast epithelium and primary invasive ductal breast carcinoma (IDC) from 177 patients by Western blot and immunohistochemical analyses, using highly specific anti-α-enolase monoclonal antibodies. A significant increase in the expression of cytoplasmic α-enolase was observed in 98% of the tumors analysed, compared to normal tissues. Nuclear MBP-1 was found in almost all the normal tissues while its expression was retained in only 35% of the tumors. Statistically significant associations were observed among the nuclear expression of MBP-1 and ErbB2 status, Ki-67 expression, node status and tumor grade. Furthermore MBP-1 expression was associated with good survival of patients with IDC.<h4>Conclusions</h4>MBP-1 functions in repressing c-myc gene expression and the results presented indicate that the loss of nuclear MBP-1 expression in a large number of IDC may be a critical step in the development and progression of breast cancer and a predictor of adverse outcome. Nuclear MBP-1 appears to be a novel and valuable histochemical marker with potential prognostic value in breast cancer.
format article
author Mariavera Lo Presti
Arianna Ferro
Flavia Contino
Claudia Mazzarella
Silvia Sbacchi
Elena Roz
Carmelo Lupo
Giovanni Perconti
Agata Giallongo
Paola Migliorini
Antonio Marrazzo
Salvatore Feo
author_facet Mariavera Lo Presti
Arianna Ferro
Flavia Contino
Claudia Mazzarella
Silvia Sbacchi
Elena Roz
Carmelo Lupo
Giovanni Perconti
Agata Giallongo
Paola Migliorini
Antonio Marrazzo
Salvatore Feo
author_sort Mariavera Lo Presti
title Myc promoter-binding protein-1 (MBP-1) is a novel potential prognostic marker in invasive ductal breast carcinoma.
title_short Myc promoter-binding protein-1 (MBP-1) is a novel potential prognostic marker in invasive ductal breast carcinoma.
title_full Myc promoter-binding protein-1 (MBP-1) is a novel potential prognostic marker in invasive ductal breast carcinoma.
title_fullStr Myc promoter-binding protein-1 (MBP-1) is a novel potential prognostic marker in invasive ductal breast carcinoma.
title_full_unstemmed Myc promoter-binding protein-1 (MBP-1) is a novel potential prognostic marker in invasive ductal breast carcinoma.
title_sort myc promoter-binding protein-1 (mbp-1) is a novel potential prognostic marker in invasive ductal breast carcinoma.
publisher Public Library of Science (PLoS)
publishDate 2010
url https://doaj.org/article/a6468528a5b34ea8ac7e8ee73efc9933
work_keys_str_mv AT mariaveralopresti mycpromoterbindingprotein1mbp1isanovelpotentialprognosticmarkerininvasiveductalbreastcarcinoma
AT ariannaferro mycpromoterbindingprotein1mbp1isanovelpotentialprognosticmarkerininvasiveductalbreastcarcinoma
AT flaviacontino mycpromoterbindingprotein1mbp1isanovelpotentialprognosticmarkerininvasiveductalbreastcarcinoma
AT claudiamazzarella mycpromoterbindingprotein1mbp1isanovelpotentialprognosticmarkerininvasiveductalbreastcarcinoma
AT silviasbacchi mycpromoterbindingprotein1mbp1isanovelpotentialprognosticmarkerininvasiveductalbreastcarcinoma
AT elenaroz mycpromoterbindingprotein1mbp1isanovelpotentialprognosticmarkerininvasiveductalbreastcarcinoma
AT carmelolupo mycpromoterbindingprotein1mbp1isanovelpotentialprognosticmarkerininvasiveductalbreastcarcinoma
AT giovanniperconti mycpromoterbindingprotein1mbp1isanovelpotentialprognosticmarkerininvasiveductalbreastcarcinoma
AT agatagiallongo mycpromoterbindingprotein1mbp1isanovelpotentialprognosticmarkerininvasiveductalbreastcarcinoma
AT paolamigliorini mycpromoterbindingprotein1mbp1isanovelpotentialprognosticmarkerininvasiveductalbreastcarcinoma
AT antoniomarrazzo mycpromoterbindingprotein1mbp1isanovelpotentialprognosticmarkerininvasiveductalbreastcarcinoma
AT salvatorefeo mycpromoterbindingprotein1mbp1isanovelpotentialprognosticmarkerininvasiveductalbreastcarcinoma
_version_ 1718424498560040960