Crayfish hemocytes develop along the granular cell lineage

Abstract Despite the central role of hemocytes in crustacean immunity, the process of hemocyte differentiation and maturation remains unclear. In some decapods, it has been proposed that the two main types of hemocytes, granular cells (GCs) and semigranular cells (SGCs), differentiate along separate...

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Autores principales:	Fang Li, Zaichao Zheng, Hongyu Li, Rongrong Fu, Limei Xu, Feng Yang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a649785d900943f7a1587c091c9e84f5
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spelling	oai:doaj.org-article:a649785d900943f7a1587c091c9e84f52021-12-02T18:02:50ZCrayfish hemocytes develop along the granular cell lineage10.1038/s41598-021-92473-92045-2322https://doaj.org/article/a649785d900943f7a1587c091c9e84f52021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92473-9https://doaj.org/toc/2045-2322Abstract Despite the central role of hemocytes in crustacean immunity, the process of hemocyte differentiation and maturation remains unclear. In some decapods, it has been proposed that the two main types of hemocytes, granular cells (GCs) and semigranular cells (SGCs), differentiate along separate lineages. However, our current findings challenge this model. By tracking newly produced hemocytes and transplanted cells, we demonstrate that almost all the circulating hemocytes of crayfish belong to the GC lineage. SGCs and GCs may represent hemocytes of different developmental stages rather than two types of fully differentiated cells. Hemocyte precursors produced by progenitor cells differentiate in the hematopoietic tissue (HPT) for 3 ~ 4 days. Immature hemocytes are released from HPT in the form of SGCs and take 1 ~ 3 months to mature in the circulation. GCs represent the terminal stage of development. They can survive for as long as 2 months. The changes in the expression pattern of marker genes during GC differentiation support our conclusions. Further analysis of hemocyte phagocytosis indicates the existence of functionally different subpopulations. These findings may reshape our understanding of crustacean hematopoiesis and may lead to reconsideration of the roles and relationship of circulating hemocytes.Fang LiZaichao ZhengHongyu LiRongrong FuLimei XuFeng YangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fang Li Zaichao Zheng Hongyu Li Rongrong Fu Limei Xu Feng Yang Crayfish hemocytes develop along the granular cell lineage
description	Abstract Despite the central role of hemocytes in crustacean immunity, the process of hemocyte differentiation and maturation remains unclear. In some decapods, it has been proposed that the two main types of hemocytes, granular cells (GCs) and semigranular cells (SGCs), differentiate along separate lineages. However, our current findings challenge this model. By tracking newly produced hemocytes and transplanted cells, we demonstrate that almost all the circulating hemocytes of crayfish belong to the GC lineage. SGCs and GCs may represent hemocytes of different developmental stages rather than two types of fully differentiated cells. Hemocyte precursors produced by progenitor cells differentiate in the hematopoietic tissue (HPT) for 3 ~ 4 days. Immature hemocytes are released from HPT in the form of SGCs and take 1 ~ 3 months to mature in the circulation. GCs represent the terminal stage of development. They can survive for as long as 2 months. The changes in the expression pattern of marker genes during GC differentiation support our conclusions. Further analysis of hemocyte phagocytosis indicates the existence of functionally different subpopulations. These findings may reshape our understanding of crustacean hematopoiesis and may lead to reconsideration of the roles and relationship of circulating hemocytes.
format	article
author	Fang Li Zaichao Zheng Hongyu Li Rongrong Fu Limei Xu Feng Yang
author_facet	Fang Li Zaichao Zheng Hongyu Li Rongrong Fu Limei Xu Feng Yang
author_sort	Fang Li
title	Crayfish hemocytes develop along the granular cell lineage
title_short	Crayfish hemocytes develop along the granular cell lineage
title_full	Crayfish hemocytes develop along the granular cell lineage
title_fullStr	Crayfish hemocytes develop along the granular cell lineage
title_full_unstemmed	Crayfish hemocytes develop along the granular cell lineage
title_sort	crayfish hemocytes develop along the granular cell lineage
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a649785d900943f7a1587c091c9e84f5
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_version_	1718378887315980288

Crayfish hemocytes develop along the granular cell lineage

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