Genetic diversity assessment of ancient mulberry (Morus spp.) in Lebanon using morphological, chemical and molecular markers (SSR and ISSR)

Lebanon has ancient mulberry trees which are the remnants of the abundant orchards that dominated its lands during the nineteenth century. Lebanese mulberry germplasm has not been assessed yet. This study aims to collect local old rainfed mulberry accessions from different geographical regions and...

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Autores principales:	Aline Kadri, Shoaib Saleh, Ahmad Elbitar, Ali Chehade
Formato:	article
Lenguaje:	EN
Publicado:	Firenze University Press 2021
Materias:	germplasm microsatellite markers morphological descriptors polymorphism principal component analysis Biology (General) QH301-705.5 Botany QK1-989
Acceso en línea:	https://doaj.org/article/a64d1fb140154a2db2ab715dd153b2e7
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spelling	oai:doaj.org-article:a64d1fb140154a2db2ab715dd153b2e72021-11-08T13:15:45ZGenetic diversity assessment of ancient mulberry (Morus spp.) in Lebanon using morphological, chemical and molecular markers (SSR and ISSR)10.36253/ahsc-83760394-61691592-1573https://doaj.org/article/a64d1fb140154a2db2ab715dd153b2e72021-06-01T00:00:00Zhttps://oaj.fupress.net/index.php/ahs/article/view/8376https://doaj.org/toc/0394-6169https://doaj.org/toc/1592-1573 Lebanon has ancient mulberry trees which are the remnants of the abundant orchards that dominated its lands during the nineteenth century. Lebanese mulberry germplasm has not been assessed yet. This study aims to collect local old rainfed mulberry accessions from different geographical regions and assess their diversity by using morphological and molecular markers (SSR and ISSR). Genetic diversity of 70 accessions of mulberry were evaluated by using 27 morphological traits. The dendrogram based on the morphological attributes showed a relative separation of the different accessions based on fruits color and taste. Molecular analysis was performed for the accessions by using selected SSR and ISSR primers. The primers marked a high discriminating power (0.7 to 0.89). The dendrogram constructed on the base of UPGMA method showed 13 different groups. The clustering patterns indicated no location nor local name specificity among mulberry accessions. The combination of SSR and ISSR primers was informative for estimating the extent of mulberry genetic diversity. It can be concluded that there is a high level of genetic diversity within mulberry trees in Lebanon. These results will be useful for mulberry germplasm management in terms of biodiversity protection and as a valuable source of gene pool for crop improvement. Aline KadriShoaib SalehAhmad ElbitarAli ChehadeFirenze University Pressarticlegermplasmmicrosatellite markersmorphological descriptorspolymorphismprincipal component analysisBiology (General)QH301-705.5BotanyQK1-989ENAdvances in Horticultural Science, Vol 35, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	germplasm microsatellite markers morphological descriptors polymorphism principal component analysis Biology (General) QH301-705.5 Botany QK1-989
spellingShingle	germplasm microsatellite markers morphological descriptors polymorphism principal component analysis Biology (General) QH301-705.5 Botany QK1-989 Aline Kadri Shoaib Saleh Ahmad Elbitar Ali Chehade Genetic diversity assessment of ancient mulberry (Morus spp.) in Lebanon using morphological, chemical and molecular markers (SSR and ISSR)
description	Lebanon has ancient mulberry trees which are the remnants of the abundant orchards that dominated its lands during the nineteenth century. Lebanese mulberry germplasm has not been assessed yet. This study aims to collect local old rainfed mulberry accessions from different geographical regions and assess their diversity by using morphological and molecular markers (SSR and ISSR). Genetic diversity of 70 accessions of mulberry were evaluated by using 27 morphological traits. The dendrogram based on the morphological attributes showed a relative separation of the different accessions based on fruits color and taste. Molecular analysis was performed for the accessions by using selected SSR and ISSR primers. The primers marked a high discriminating power (0.7 to 0.89). The dendrogram constructed on the base of UPGMA method showed 13 different groups. The clustering patterns indicated no location nor local name specificity among mulberry accessions. The combination of SSR and ISSR primers was informative for estimating the extent of mulberry genetic diversity. It can be concluded that there is a high level of genetic diversity within mulberry trees in Lebanon. These results will be useful for mulberry germplasm management in terms of biodiversity protection and as a valuable source of gene pool for crop improvement.
format	article
author	Aline Kadri Shoaib Saleh Ahmad Elbitar Ali Chehade
author_facet	Aline Kadri Shoaib Saleh Ahmad Elbitar Ali Chehade
author_sort	Aline Kadri
title	Genetic diversity assessment of ancient mulberry (Morus spp.) in Lebanon using morphological, chemical and molecular markers (SSR and ISSR)
title_short	Genetic diversity assessment of ancient mulberry (Morus spp.) in Lebanon using morphological, chemical and molecular markers (SSR and ISSR)
title_full	Genetic diversity assessment of ancient mulberry (Morus spp.) in Lebanon using morphological, chemical and molecular markers (SSR and ISSR)
title_fullStr	Genetic diversity assessment of ancient mulberry (Morus spp.) in Lebanon using morphological, chemical and molecular markers (SSR and ISSR)
title_full_unstemmed	Genetic diversity assessment of ancient mulberry (Morus spp.) in Lebanon using morphological, chemical and molecular markers (SSR and ISSR)
title_sort	genetic diversity assessment of ancient mulberry (morus spp.) in lebanon using morphological, chemical and molecular markers (ssr and issr)
publisher	Firenze University Press
publishDate	2021
url	https://doaj.org/article/a64d1fb140154a2db2ab715dd153b2e7
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Genetic diversity assessment of ancient mulberry (Morus spp.) in Lebanon using morphological, chemical and molecular markers (SSR and ISSR)

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