The maintenance of microbial community in human fecal samples by a cost effective preservation buffer

Abstract In the burgeoning microbiome field, powerful sequencing approaches and accompanied bioanalytical methods have made tremendous contributions to the discoveries of breakthroughs, which favor to unravel the intimate interplay between gut microbiota and human health. The proper preservation of...

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Autores principales:	Chongming Wu, Tianda Chen, Wenyi Xu, Tingting Zhang, Yuwei Pei, Yanan Yang, Fang Zhang, Hao Guo, Qingshi Wang, Li Wang, Bowen Zhao
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a651a3c12a0149c4982938019c6c9d2d
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spelling	oai:doaj.org-article:a651a3c12a0149c4982938019c6c9d2d2021-12-02T16:10:37ZThe maintenance of microbial community in human fecal samples by a cost effective preservation buffer10.1038/s41598-021-92869-72045-2322https://doaj.org/article/a651a3c12a0149c4982938019c6c9d2d2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-92869-7https://doaj.org/toc/2045-2322Abstract In the burgeoning microbiome field, powerful sequencing approaches and accompanied bioanalytical methods have made tremendous contributions to the discoveries of breakthroughs, which favor to unravel the intimate interplay between gut microbiota and human health. The proper preservation of samples before being processed is essential to guarantee the authenticity and reliability of microbiome studies. Hence, the development of preservation methods is extremely important to hold samples eligible for the consequent analysis, especially population cohort-based investigations or those spanning species or geography, which frequently facing difficulties in suppling freezing conditions. Although there are several commercial products available, the exploration of cost-efficient and ready-to-use preservation methods are still in a large demand. Here, we performed shotgun metagenomic sequencing and demonstrated that microbial consortia in human fecal samples were substantially preserved within a temporary storage of 4 h, independent of the storage temperature. We also verified a previous reported self-made preservation buffer (PB buffer) could not only preserve fecal microbiota at room temperature up to 4 weeks but also enable samples to endure a high temperature condition which mimics temperature variations in summer logistics. Moreover, PB buffer exhibited suitability for human saliva as well. Collectively, PB buffer may be a valuable choice to stabilize samples if neither freezing facilities nor liquid nitrogen is available.Chongming WuTianda ChenWenyi XuTingting ZhangYuwei PeiYanan YangFang ZhangHao GuoQingshi WangLi WangBowen ZhaoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chongming Wu Tianda Chen Wenyi Xu Tingting Zhang Yuwei Pei Yanan Yang Fang Zhang Hao Guo Qingshi Wang Li Wang Bowen Zhao The maintenance of microbial community in human fecal samples by a cost effective preservation buffer
description	Abstract In the burgeoning microbiome field, powerful sequencing approaches and accompanied bioanalytical methods have made tremendous contributions to the discoveries of breakthroughs, which favor to unravel the intimate interplay between gut microbiota and human health. The proper preservation of samples before being processed is essential to guarantee the authenticity and reliability of microbiome studies. Hence, the development of preservation methods is extremely important to hold samples eligible for the consequent analysis, especially population cohort-based investigations or those spanning species or geography, which frequently facing difficulties in suppling freezing conditions. Although there are several commercial products available, the exploration of cost-efficient and ready-to-use preservation methods are still in a large demand. Here, we performed shotgun metagenomic sequencing and demonstrated that microbial consortia in human fecal samples were substantially preserved within a temporary storage of 4 h, independent of the storage temperature. We also verified a previous reported self-made preservation buffer (PB buffer) could not only preserve fecal microbiota at room temperature up to 4 weeks but also enable samples to endure a high temperature condition which mimics temperature variations in summer logistics. Moreover, PB buffer exhibited suitability for human saliva as well. Collectively, PB buffer may be a valuable choice to stabilize samples if neither freezing facilities nor liquid nitrogen is available.
format	article
author	Chongming Wu Tianda Chen Wenyi Xu Tingting Zhang Yuwei Pei Yanan Yang Fang Zhang Hao Guo Qingshi Wang Li Wang Bowen Zhao
author_facet	Chongming Wu Tianda Chen Wenyi Xu Tingting Zhang Yuwei Pei Yanan Yang Fang Zhang Hao Guo Qingshi Wang Li Wang Bowen Zhao
author_sort	Chongming Wu
title	The maintenance of microbial community in human fecal samples by a cost effective preservation buffer
title_short	The maintenance of microbial community in human fecal samples by a cost effective preservation buffer
title_full	The maintenance of microbial community in human fecal samples by a cost effective preservation buffer
title_fullStr	The maintenance of microbial community in human fecal samples by a cost effective preservation buffer
title_full_unstemmed	The maintenance of microbial community in human fecal samples by a cost effective preservation buffer
title_sort	maintenance of microbial community in human fecal samples by a cost effective preservation buffer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a651a3c12a0149c4982938019c6c9d2d
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The maintenance of microbial community in human fecal samples by a cost effective preservation buffer

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