High genetic diversity among community-associated Staphylococcus aureus in Europe: results from a multicenter study.

<h4>Background</h4>Several studies have addressed the epidemiology of community-associated Staphylococcus aureus (CA-SA) in Europe; nonetheless, a comprehensive perspective remains unclear. In this study, we aimed to describe the population structure of CA-SA and to shed light on the ori...

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Autores principales:	Joana Rolo, Maria Miragaia, Agata Turlej-Rogacka, Joanna Empel, Ons Bouchami, Nuno A Faria, Ana Tavares, Waleria Hryniewicz, Ad C Fluit, Hermínia de Lencastre, CONCORD Working Group
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a653464a3f694518b30b81d14afb51dd
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spelling	oai:doaj.org-article:a653464a3f694518b30b81d14afb51dd2021-11-18T07:20:37ZHigh genetic diversity among community-associated Staphylococcus aureus in Europe: results from a multicenter study.1932-620310.1371/journal.pone.0034768https://doaj.org/article/a653464a3f694518b30b81d14afb51dd2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22558099/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Several studies have addressed the epidemiology of community-associated Staphylococcus aureus (CA-SA) in Europe; nonetheless, a comprehensive perspective remains unclear. In this study, we aimed to describe the population structure of CA-SA and to shed light on the origin of methicillin-resistant S. aureus (MRSA) in this continent.<h4>Methods and findings</h4>A total of 568 colonization and infection isolates, comprising both MRSA and methicillin-susceptible S. aureus (MSSA), were recovered in 16 European countries, from community and community-onset infections. The genetic background of isolates was characterized by molecular typing techniques (spa typing, pulsed-field gel electrophoresis and multilocus sequence typing) and the presence of PVL and ACME was tested by PCR. MRSA were further characterized by SCCmec typing. We found that 59% of all isolates were associated with community-associated clones. Most MRSA were related with USA300 (ST8-IVa and variants) (40%), followed by the European clone (ST80-IVc and derivatives) (28%) and the Taiwan clone (ST59-IVa and related clonal types) (15%). A total of 83% of MRSA carried Panton-Valentine leukocidin (PVL) and 14% carried the arginine catabolic mobile element (ACME). Surprisingly, we found a high genetic diversity among MRSA clonal types (ST-SCCmec), Simpson's index of diversity = 0.852 (0.788-0.916). Specifically, about half of the isolates carried novel associations between genetic background and SCCmec. Analysis by BURP showed that some CA-MSSA and CA-MRSA isolates were highly related, suggesting a probable local acquisition/loss of SCCmec.<h4>Conclusions</h4>Our results imply that CA-MRSA origin, epidemiology and population structure in Europe is very dissimilar from that of USA.Joana RoloMaria MiragaiaAgata Turlej-RogackaJoanna EmpelOns BouchamiNuno A FariaAna TavaresWaleria HryniewiczAd C FluitHermínia de LencastreCONCORD Working GroupPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e34768 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Joana Rolo Maria Miragaia Agata Turlej-Rogacka Joanna Empel Ons Bouchami Nuno A Faria Ana Tavares Waleria Hryniewicz Ad C Fluit Hermínia de Lencastre CONCORD Working Group High genetic diversity among community-associated Staphylococcus aureus in Europe: results from a multicenter study.
description	<h4>Background</h4>Several studies have addressed the epidemiology of community-associated Staphylococcus aureus (CA-SA) in Europe; nonetheless, a comprehensive perspective remains unclear. In this study, we aimed to describe the population structure of CA-SA and to shed light on the origin of methicillin-resistant S. aureus (MRSA) in this continent.<h4>Methods and findings</h4>A total of 568 colonization and infection isolates, comprising both MRSA and methicillin-susceptible S. aureus (MSSA), were recovered in 16 European countries, from community and community-onset infections. The genetic background of isolates was characterized by molecular typing techniques (spa typing, pulsed-field gel electrophoresis and multilocus sequence typing) and the presence of PVL and ACME was tested by PCR. MRSA were further characterized by SCCmec typing. We found that 59% of all isolates were associated with community-associated clones. Most MRSA were related with USA300 (ST8-IVa and variants) (40%), followed by the European clone (ST80-IVc and derivatives) (28%) and the Taiwan clone (ST59-IVa and related clonal types) (15%). A total of 83% of MRSA carried Panton-Valentine leukocidin (PVL) and 14% carried the arginine catabolic mobile element (ACME). Surprisingly, we found a high genetic diversity among MRSA clonal types (ST-SCCmec), Simpson's index of diversity = 0.852 (0.788-0.916). Specifically, about half of the isolates carried novel associations between genetic background and SCCmec. Analysis by BURP showed that some CA-MSSA and CA-MRSA isolates were highly related, suggesting a probable local acquisition/loss of SCCmec.<h4>Conclusions</h4>Our results imply that CA-MRSA origin, epidemiology and population structure in Europe is very dissimilar from that of USA.
format	article
author	Joana Rolo Maria Miragaia Agata Turlej-Rogacka Joanna Empel Ons Bouchami Nuno A Faria Ana Tavares Waleria Hryniewicz Ad C Fluit Hermínia de Lencastre CONCORD Working Group
author_facet	Joana Rolo Maria Miragaia Agata Turlej-Rogacka Joanna Empel Ons Bouchami Nuno A Faria Ana Tavares Waleria Hryniewicz Ad C Fluit Hermínia de Lencastre CONCORD Working Group
author_sort	Joana Rolo
title	High genetic diversity among community-associated Staphylococcus aureus in Europe: results from a multicenter study.
title_short	High genetic diversity among community-associated Staphylococcus aureus in Europe: results from a multicenter study.
title_full	High genetic diversity among community-associated Staphylococcus aureus in Europe: results from a multicenter study.
title_fullStr	High genetic diversity among community-associated Staphylococcus aureus in Europe: results from a multicenter study.
title_full_unstemmed	High genetic diversity among community-associated Staphylococcus aureus in Europe: results from a multicenter study.
title_sort	high genetic diversity among community-associated staphylococcus aureus in europe: results from a multicenter study.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a653464a3f694518b30b81d14afb51dd
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High genetic diversity among community-associated Staphylococcus aureus in Europe: results from a multicenter study.

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