Generation, annotation and analysis of first large-scale expressed sequence tags from developing fiber of Gossypium barbadense L.

<h4>Background</h4>Cotton fiber is the world's leading natural fiber used in the manufacture of textiles. Gossypium is also the model plant in the study of polyploidization, evolution, cell elongation, cell wall development, and cellulose biosynthesis. G. barbadense L. is an ideal c...

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Autores principales: Daojun Yuan, Lili Tu, Xianlong Zhang
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Publicado: Public Library of Science (PLoS) 2011
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spelling oai:doaj.org-article:a65addcc085644b191ed49358d3eade62021-11-18T06:49:08ZGeneration, annotation and analysis of first large-scale expressed sequence tags from developing fiber of Gossypium barbadense L.1932-620310.1371/journal.pone.0022758https://doaj.org/article/a65addcc085644b191ed49358d3eade62011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21829504/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Cotton fiber is the world's leading natural fiber used in the manufacture of textiles. Gossypium is also the model plant in the study of polyploidization, evolution, cell elongation, cell wall development, and cellulose biosynthesis. G. barbadense L. is an ideal candidate for providing new genetic variations useful to improve fiber quality for its superior properties. However, little is known about fiber development mechanisms of G. barbadense and only a few molecular resources are available in GenBank.<h4>Methodology and principal findings</h4>In total, 10,979 high-quality expressed sequence tags (ESTs) were generated from a normalized fiber cDNA library of G. barbadense. The ESTs were clustered and assembled into 5852 unigenes, consisting of 1492 contigs and 4360 singletons. The blastx result showed 2165 unigenes with significant similarity to known genes and 2687 unigenes with significant similarity to genes of predicted proteins. Functional classification revealed that unigenes were abundant in the functions of binding, catalytic activity, and metabolic pathways of carbohydrate, amino acid, energy, and lipids. The function motif/domain-related cytoskeleton and redox homeostasis were enriched. Among the 5852 unigenes, 282 and 736 unigenes were identified as potential cell wall biosynthesis and transcription factors, respectively. Furthermore, the relationships among cotton species or between cotton and other model plant systems were analyzed. Some putative species-specific unigenes of G. barbadense were highlighted.<h4>Conclusions/significance</h4>The ESTs generated in this study are from the first large-scale EST project for G. barbadense and significantly enhance the number of G. barbadense ESTs in public databases. This knowledge will contribute to cotton improvements by studying fiber development mechanisms of G. barbadense, establishing a breeding program using marker-assisted selection, and discovering candidate genes related to important agronomic traits of cotton through oligonucleotide array. Our work will also provide important resources for comparative genomics, polyploidization, and genome evolution among Gossypium species.Daojun YuanLili TuXianlong ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 7, p e22758 (2011)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Daojun Yuan
Lili Tu
Xianlong Zhang
Generation, annotation and analysis of first large-scale expressed sequence tags from developing fiber of Gossypium barbadense L.
description <h4>Background</h4>Cotton fiber is the world's leading natural fiber used in the manufacture of textiles. Gossypium is also the model plant in the study of polyploidization, evolution, cell elongation, cell wall development, and cellulose biosynthesis. G. barbadense L. is an ideal candidate for providing new genetic variations useful to improve fiber quality for its superior properties. However, little is known about fiber development mechanisms of G. barbadense and only a few molecular resources are available in GenBank.<h4>Methodology and principal findings</h4>In total, 10,979 high-quality expressed sequence tags (ESTs) were generated from a normalized fiber cDNA library of G. barbadense. The ESTs were clustered and assembled into 5852 unigenes, consisting of 1492 contigs and 4360 singletons. The blastx result showed 2165 unigenes with significant similarity to known genes and 2687 unigenes with significant similarity to genes of predicted proteins. Functional classification revealed that unigenes were abundant in the functions of binding, catalytic activity, and metabolic pathways of carbohydrate, amino acid, energy, and lipids. The function motif/domain-related cytoskeleton and redox homeostasis were enriched. Among the 5852 unigenes, 282 and 736 unigenes were identified as potential cell wall biosynthesis and transcription factors, respectively. Furthermore, the relationships among cotton species or between cotton and other model plant systems were analyzed. Some putative species-specific unigenes of G. barbadense were highlighted.<h4>Conclusions/significance</h4>The ESTs generated in this study are from the first large-scale EST project for G. barbadense and significantly enhance the number of G. barbadense ESTs in public databases. This knowledge will contribute to cotton improvements by studying fiber development mechanisms of G. barbadense, establishing a breeding program using marker-assisted selection, and discovering candidate genes related to important agronomic traits of cotton through oligonucleotide array. Our work will also provide important resources for comparative genomics, polyploidization, and genome evolution among Gossypium species.
format article
author Daojun Yuan
Lili Tu
Xianlong Zhang
author_facet Daojun Yuan
Lili Tu
Xianlong Zhang
author_sort Daojun Yuan
title Generation, annotation and analysis of first large-scale expressed sequence tags from developing fiber of Gossypium barbadense L.
title_short Generation, annotation and analysis of first large-scale expressed sequence tags from developing fiber of Gossypium barbadense L.
title_full Generation, annotation and analysis of first large-scale expressed sequence tags from developing fiber of Gossypium barbadense L.
title_fullStr Generation, annotation and analysis of first large-scale expressed sequence tags from developing fiber of Gossypium barbadense L.
title_full_unstemmed Generation, annotation and analysis of first large-scale expressed sequence tags from developing fiber of Gossypium barbadense L.
title_sort generation, annotation and analysis of first large-scale expressed sequence tags from developing fiber of gossypium barbadense l.
publisher Public Library of Science (PLoS)
publishDate 2011
url https://doaj.org/article/a65addcc085644b191ed49358d3eade6
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AT lilitu generationannotationandanalysisoffirstlargescaleexpressedsequencetagsfromdevelopingfiberofgossypiumbarbadensel
AT xianlongzhang generationannotationandanalysisoffirstlargescaleexpressedsequencetagsfromdevelopingfiberofgossypiumbarbadensel
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