Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO2 nanotube layers fabricated by lyophilization following trehalose addition

Xiaochen Zhang,1 Zhiyuan Zhang,1 Gang Shen,2 Jun Zhao2 1Department of Oral and Maxillofacial Surgery, 2Department of Orthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of...

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Autores principales:	Zhang X, Zhang Z, Shen G, Zhao J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2016
Materias:	Osteogenesis Anti-inflammation TiO2 nanotube layers Lenti-BMP-2 Lyophilization Trehalose Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a660e676b0974e64a809a1133fa8c596
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spelling	oai:doaj.org-article:a660e676b0974e64a809a1133fa8c5962021-12-02T05:02:05ZEnhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO2 nanotube layers fabricated by lyophilization following trehalose addition1178-2013https://doaj.org/article/a660e676b0974e64a809a1133fa8c5962016-01-01T00:00:00Zhttps://www.dovepress.com/enhanced-osteogenic-activity-and-anti-inflammatory-properties-of-lenti-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Xiaochen Zhang,1 Zhiyuan Zhang,1 Gang Shen,2 Jun Zhao2 1Department of Oral and Maxillofacial Surgery, 2Department of Orthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China Abstract: To enhance biocompatibility and osseointegration between titanium implants and surrounding bone tissue, numerous efforts have been made to modify the surface topography and composition of Ti implants. In this paper, Lenti-BMP-2-loaded TiO2 nanotube coatings were fabricated by lyophilization in the presence of trehalose to functionalize the surface. We characterized TiO2 nanotube layers in terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration in vivo. The anodized TiO2 nanotube surfaces exhibited an amorphous glassy matrix perpendicular to the Ti surface. Both Lenti-BMP-2 and trehalose showed sustained release over the course of 8 days. Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation. In addition, Lyo-Tre-Lenti-BMP-2 nanotubes effectively inhibited lipopolysaccharide-induced interleukin-1β and tumor necrosis factor-α production. In vivo, the formulation also promoted osseointegration. This study presents a promising new method for surface-modifying biomedical Ti-based implants to simultaneously enhance their osteogenic potential and anti-inflammatory properties, which can better satisfy clinical needs. Keywords: osteogenesis, anti-inflammation, TiO2 nanotube layers, Lenti-BMP-2, lyophilization, trehalose Zhang XZhang ZShen GZhao JDove Medical PressarticleOsteogenesisAnti-inflammationTiO2 nanotube layersLenti-BMP-2LyophilizationTrehaloseMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2016, Iss Issue 1, Pp 429-439 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Osteogenesis Anti-inflammation TiO2 nanotube layers Lenti-BMP-2 Lyophilization Trehalose Medicine (General) R5-920
spellingShingle	Osteogenesis Anti-inflammation TiO2 nanotube layers Lenti-BMP-2 Lyophilization Trehalose Medicine (General) R5-920 Zhang X Zhang Z Shen G Zhao J Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO2 nanotube layers fabricated by lyophilization following trehalose addition
description	Xiaochen Zhang,1 Zhiyuan Zhang,1 Gang Shen,2 Jun Zhao2 1Department of Oral and Maxillofacial Surgery, 2Department of Orthodontics, College of Stomatology, Ninth People’s Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, People’s Republic of China Abstract: To enhance biocompatibility and osseointegration between titanium implants and surrounding bone tissue, numerous efforts have been made to modify the surface topography and composition of Ti implants. In this paper, Lenti-BMP-2-loaded TiO2 nanotube coatings were fabricated by lyophilization in the presence of trehalose to functionalize the surface. We characterized TiO2 nanotube layers in terms of the following: surface morphology; Lenti-BMP-2 and trehalose release; their ability to induce osteogenesis, proliferation, and anti-inflammation in vitro; and osseointegration in vivo. The anodized TiO2 nanotube surfaces exhibited an amorphous glassy matrix perpendicular to the Ti surface. Both Lenti-BMP-2 and trehalose showed sustained release over the course of 8 days. Results from real-time quantitative polymerase chain reaction studies demonstrated that lyophilized Lenti-BMP-2/TiO2 nanotubes constructed with trehalose (Lyo-Tre-Lenti-BMP-2) significantly promoted osteogenic differentiation of bone marrow stromal cells but not their proliferation. In addition, Lyo-Tre-Lenti-BMP-2 nanotubes effectively inhibited lipopolysaccharide-induced interleukin-1β and tumor necrosis factor-α production. In vivo, the formulation also promoted osseointegration. This study presents a promising new method for surface-modifying biomedical Ti-based implants to simultaneously enhance their osteogenic potential and anti-inflammatory properties, which can better satisfy clinical needs. Keywords: osteogenesis, anti-inflammation, TiO2 nanotube layers, Lenti-BMP-2, lyophilization, trehalose
format	article
author	Zhang X Zhang Z Shen G Zhao J
author_facet	Zhang X Zhang Z Shen G Zhao J
author_sort	Zhang X
title	Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO2 nanotube layers fabricated by lyophilization following trehalose addition
title_short	Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO2 nanotube layers fabricated by lyophilization following trehalose addition
title_full	Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO2 nanotube layers fabricated by lyophilization following trehalose addition
title_fullStr	Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO2 nanotube layers fabricated by lyophilization following trehalose addition
title_full_unstemmed	Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO2 nanotube layers fabricated by lyophilization following trehalose addition
title_sort	enhanced osteogenic activity and anti-inflammatory properties of lenti-bmp-2-loaded tio2 nanotube layers fabricated by lyophilization following trehalose addition
publisher	Dove Medical Press
publishDate	2016
url	https://doaj.org/article/a660e676b0974e64a809a1133fa8c596
work_keys_str_mv	AT zhangx enhancedosteogenicactivityandantiinflammatorypropertiesoflentibmp2loadedtio2nanotubelayersfabricatedbylyophilizationfollowingtrehaloseaddition AT zhangz enhancedosteogenicactivityandantiinflammatorypropertiesoflentibmp2loadedtio2nanotubelayersfabricatedbylyophilizationfollowingtrehaloseaddition AT sheng enhancedosteogenicactivityandantiinflammatorypropertiesoflentibmp2loadedtio2nanotubelayersfabricatedbylyophilizationfollowingtrehaloseaddition AT zhaoj enhancedosteogenicactivityandantiinflammatorypropertiesoflentibmp2loadedtio2nanotubelayersfabricatedbylyophilizationfollowingtrehaloseaddition
_version_	1718400784241000448

Enhanced osteogenic activity and anti-inflammatory properties of Lenti-BMP-2-loaded TiO2 nanotube layers fabricated by lyophilization following trehalose addition

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