fam20C participates in the shell formation in the pearl oyster, Pinctada fucata

Abstract Kinase-family with sequence similarity 20, member C (Fam20C) is a protein kinase, which can phosphorylate biomineralization related proteins in vertebrate animals. However, the function of Fam20C in invertebrate animals especially the role in biomineralization is still unknown. Herein, we c...

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Autores principales:	Jinzhe Du, Chuang Liu, Guangrui Xu, Jun Xie, Liping Xie, Rongqing Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6614e02080f420d8e37058201e7cdb0
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spelling	oai:doaj.org-article:a6614e02080f420d8e37058201e7cdb02021-12-02T11:41:02Zfam20C participates in the shell formation in the pearl oyster, Pinctada fucata10.1038/s41598-018-21797-w2045-2322https://doaj.org/article/a6614e02080f420d8e37058201e7cdb02018-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-21797-whttps://doaj.org/toc/2045-2322Abstract Kinase-family with sequence similarity 20, member C (Fam20C) is a protein kinase, which can phosphorylate biomineralization related proteins in vertebrate animals. However, the function of Fam20C in invertebrate animals especially the role in biomineralization is still unknown. Herein, we cloned the cDNA of fam20C from the pearl oyster, Pinctada fucata. It is showed that the expression of fam20C in the mantle edge was much higher than other tissues. In situ hybridization showed that fam20C was expressed mostly in the outer epithelial cells of the middle fold, indicating it may play important roles in the shell formation. Besides, fam20C expression increased greatly in the D-shape stage of pearl oyster development, when the shell was first formed. During the shell repair process, the expression level of fam20C increased 1.5 times at 6 h after shell notching. Knockdown of fam20C in vivo by RNA interference resulted in abnormally stacking of calcium carbonate crystals at the edges of nacre tablets, showing direct evidence that fam20C participates in the shell formation. This study provides an insight into the role of kinase protein in the shell formation in mollusk and broaden our understanding of biomineralization mechanism.Jinzhe DuChuang LiuGuangrui XuJun XieLiping XieRongqing ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jinzhe Du Chuang Liu Guangrui Xu Jun Xie Liping Xie Rongqing Zhang fam20C participates in the shell formation in the pearl oyster, Pinctada fucata
description	Abstract Kinase-family with sequence similarity 20, member C (Fam20C) is a protein kinase, which can phosphorylate biomineralization related proteins in vertebrate animals. However, the function of Fam20C in invertebrate animals especially the role in biomineralization is still unknown. Herein, we cloned the cDNA of fam20C from the pearl oyster, Pinctada fucata. It is showed that the expression of fam20C in the mantle edge was much higher than other tissues. In situ hybridization showed that fam20C was expressed mostly in the outer epithelial cells of the middle fold, indicating it may play important roles in the shell formation. Besides, fam20C expression increased greatly in the D-shape stage of pearl oyster development, when the shell was first formed. During the shell repair process, the expression level of fam20C increased 1.5 times at 6 h after shell notching. Knockdown of fam20C in vivo by RNA interference resulted in abnormally stacking of calcium carbonate crystals at the edges of nacre tablets, showing direct evidence that fam20C participates in the shell formation. This study provides an insight into the role of kinase protein in the shell formation in mollusk and broaden our understanding of biomineralization mechanism.
format	article
author	Jinzhe Du Chuang Liu Guangrui Xu Jun Xie Liping Xie Rongqing Zhang
author_facet	Jinzhe Du Chuang Liu Guangrui Xu Jun Xie Liping Xie Rongqing Zhang
author_sort	Jinzhe Du
title	fam20C participates in the shell formation in the pearl oyster, Pinctada fucata
title_short	fam20C participates in the shell formation in the pearl oyster, Pinctada fucata
title_full	fam20C participates in the shell formation in the pearl oyster, Pinctada fucata
title_fullStr	fam20C participates in the shell formation in the pearl oyster, Pinctada fucata
title_full_unstemmed	fam20C participates in the shell formation in the pearl oyster, Pinctada fucata
title_sort	fam20c participates in the shell formation in the pearl oyster, pinctada fucata
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/a6614e02080f420d8e37058201e7cdb0
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fam20C participates in the shell formation in the pearl oyster, Pinctada fucata

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