Lessons from Epstein-Barr virus DNA detection in cerebrospinal fluid as a diagnostic tool for EBV-induced central nervous system dysfunction among HIV-positive patients

Polymerase chain reaction (PCR) analysis of Epstein-Barr virus (EBV) DNA in cerebrospinal fluid (CSF) remains vital for evaluating active EBV infection involving the central nervous system (CNS). CSF EBV DNA was often found in conjunction with other microbial infection affecting the CNS among patien...

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Autores principales:	Yanli Wang, Jun Yang, Ying Wen
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Epstein-Barr virus (EBV) detection Central nervous system Human immunodeficiency virus Acquired immune deficiency syndrome Cerebrospinal fluid Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/a66364cc481b484ca67b9c2ac7208c65
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spelling	oai:doaj.org-article:a66364cc481b484ca67b9c2ac7208c652021-11-14T04:30:27ZLessons from Epstein-Barr virus DNA detection in cerebrospinal fluid as a diagnostic tool for EBV-induced central nervous system dysfunction among HIV-positive patients0753-332210.1016/j.biopha.2021.112392https://doaj.org/article/a66364cc481b484ca67b9c2ac7208c652022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0753332221011781https://doaj.org/toc/0753-3322Polymerase chain reaction (PCR) analysis of Epstein-Barr virus (EBV) DNA in cerebrospinal fluid (CSF) remains vital for evaluating active EBV infection involving the central nervous system (CNS). CSF EBV DNA was often found in conjunction with other microbial infection affecting the CNS among patients infected with human immunodeficiency virus (HIV). Sometimes CSF EBV DNA is detectable in patients without neurological symptoms. This review focused on the clinical and laboratory features of CNS EBV infection among patients with HIV, and discussed various types of EBV-associated CNS infections, and predominant neoplasms involving CNS such as primary central nervous system lymphoma (PCNSL), CNS-non-Hodgkin's lymphoma, smooth muscle tumors and leiomyosarcomas, EBV encephalitis or myelitis, EBV meningitis and EBV coinfection with other causative agents were also included. Furthermore, the metagenomic next-generation sequencing technique with high sensitivity for the detection of pathogenic coinfection in the CSF were also reviewed. We concluded that CSF EBV-DNA detection with high sensitivity and specificity could be a useful diagnostic tool for CNS lymphoma among HIV patients; however, it is still unknown for other CNS diseases. We further summarized and conclude that positive CSF EBV-DNA detection combined with specific brain focal lesions could be a minimally invasive method to diagnose PCNSL. The occurrence of positive CSF EBV-DNA was influenced by PCR detection limit, PCR methods, immunocompromised status, the possible influence of anti-herpetic therapy and anti-HIV therapy, and the size and location of a tumor mass. Uniform PCR methods as vital diagnostic tools and optimal EBV-DNA load threshold need to be established.Yanli WangJun YangYing WenElsevierarticleEpstein-Barr virus (EBV) detectionCentral nervous systemHuman immunodeficiency virusAcquired immune deficiency syndromeCerebrospinal fluidTherapeutics. PharmacologyRM1-950ENBiomedicine & Pharmacotherapy, Vol 145, Iss , Pp 112392- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Epstein-Barr virus (EBV) detection Central nervous system Human immunodeficiency virus Acquired immune deficiency syndrome Cerebrospinal fluid Therapeutics. Pharmacology RM1-950
spellingShingle	Epstein-Barr virus (EBV) detection Central nervous system Human immunodeficiency virus Acquired immune deficiency syndrome Cerebrospinal fluid Therapeutics. Pharmacology RM1-950 Yanli Wang Jun Yang Ying Wen Lessons from Epstein-Barr virus DNA detection in cerebrospinal fluid as a diagnostic tool for EBV-induced central nervous system dysfunction among HIV-positive patients
description	Polymerase chain reaction (PCR) analysis of Epstein-Barr virus (EBV) DNA in cerebrospinal fluid (CSF) remains vital for evaluating active EBV infection involving the central nervous system (CNS). CSF EBV DNA was often found in conjunction with other microbial infection affecting the CNS among patients infected with human immunodeficiency virus (HIV). Sometimes CSF EBV DNA is detectable in patients without neurological symptoms. This review focused on the clinical and laboratory features of CNS EBV infection among patients with HIV, and discussed various types of EBV-associated CNS infections, and predominant neoplasms involving CNS such as primary central nervous system lymphoma (PCNSL), CNS-non-Hodgkin's lymphoma, smooth muscle tumors and leiomyosarcomas, EBV encephalitis or myelitis, EBV meningitis and EBV coinfection with other causative agents were also included. Furthermore, the metagenomic next-generation sequencing technique with high sensitivity for the detection of pathogenic coinfection in the CSF were also reviewed. We concluded that CSF EBV-DNA detection with high sensitivity and specificity could be a useful diagnostic tool for CNS lymphoma among HIV patients; however, it is still unknown for other CNS diseases. We further summarized and conclude that positive CSF EBV-DNA detection combined with specific brain focal lesions could be a minimally invasive method to diagnose PCNSL. The occurrence of positive CSF EBV-DNA was influenced by PCR detection limit, PCR methods, immunocompromised status, the possible influence of anti-herpetic therapy and anti-HIV therapy, and the size and location of a tumor mass. Uniform PCR methods as vital diagnostic tools and optimal EBV-DNA load threshold need to be established.
format	article
author	Yanli Wang Jun Yang Ying Wen
author_facet	Yanli Wang Jun Yang Ying Wen
author_sort	Yanli Wang
title	Lessons from Epstein-Barr virus DNA detection in cerebrospinal fluid as a diagnostic tool for EBV-induced central nervous system dysfunction among HIV-positive patients
title_short	Lessons from Epstein-Barr virus DNA detection in cerebrospinal fluid as a diagnostic tool for EBV-induced central nervous system dysfunction among HIV-positive patients
title_full	Lessons from Epstein-Barr virus DNA detection in cerebrospinal fluid as a diagnostic tool for EBV-induced central nervous system dysfunction among HIV-positive patients
title_fullStr	Lessons from Epstein-Barr virus DNA detection in cerebrospinal fluid as a diagnostic tool for EBV-induced central nervous system dysfunction among HIV-positive patients
title_full_unstemmed	Lessons from Epstein-Barr virus DNA detection in cerebrospinal fluid as a diagnostic tool for EBV-induced central nervous system dysfunction among HIV-positive patients
title_sort	lessons from epstein-barr virus dna detection in cerebrospinal fluid as a diagnostic tool for ebv-induced central nervous system dysfunction among hiv-positive patients
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/a66364cc481b484ca67b9c2ac7208c65
work_keys_str_mv	AT yanliwang lessonsfromepsteinbarrvirusdnadetectionincerebrospinalfluidasadiagnostictoolforebvinducedcentralnervoussystemdysfunctionamonghivpositivepatients AT junyang lessonsfromepsteinbarrvirusdnadetectionincerebrospinalfluidasadiagnostictoolforebvinducedcentralnervoussystemdysfunctionamonghivpositivepatients AT yingwen lessonsfromepsteinbarrvirusdnadetectionincerebrospinalfluidasadiagnostictoolforebvinducedcentralnervoussystemdysfunctionamonghivpositivepatients
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Lessons from Epstein-Barr virus DNA detection in cerebrospinal fluid as a diagnostic tool for EBV-induced central nervous system dysfunction among HIV-positive patients

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