Investigation of the Expression of Inflammatory Markers in Oral Biofilm Samples in Patients with Systemic Scleroderma and the Association with Clinical Periodontal Parameters—A Preliminary Study

Background: Systemic scleroderma (SSc) has multiple orofacial effects. The aim of this study was to analyze the expression of inflammatory mediators in biofilm samples. It was hypothesized that different expression levels and clinical associations might be drawn. Methods: A total of 39 biofilm sampl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Mayte Buchbender, Amelie Lugenbühl, Jakob Fehlhofer, Christian Kirschneck, Jutta Ries, Rainer Lutz, Michael Sticherling, Marco Rainer Kesting
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	biofilm samples expression levels of cytokines periodontal parameters systemic scleroderma (SSc) Science Q
Acceso en línea:	https://doaj.org/article/a6645ad5db5b47a2b8eb3e87222e34f2
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a6645ad5db5b47a2b8eb3e87222e34f2
record_format	dspace
spelling	oai:doaj.org-article:a6645ad5db5b47a2b8eb3e87222e34f22021-11-25T18:10:38ZInvestigation of the Expression of Inflammatory Markers in Oral Biofilm Samples in Patients with Systemic Scleroderma and the Association with Clinical Periodontal Parameters—A Preliminary Study10.3390/life111111452075-1729https://doaj.org/article/a6645ad5db5b47a2b8eb3e87222e34f22021-10-01T00:00:00Zhttps://www.mdpi.com/2075-1729/11/11/1145https://doaj.org/toc/2075-1729Background: Systemic scleroderma (SSc) has multiple orofacial effects. The aim of this study was to analyze the expression of inflammatory mediators in biofilm samples. It was hypothesized that different expression levels and clinical associations might be drawn. Methods: A total of 39 biofilm samples from group 1 = SSc and group 2 = healthy control were examined for the expression levels of interleukin (IL)-2,-6, and -10; matrix metalloprotease (MMP)-9; and surface antigens CD90 and CD34 by quantitative real-time PCR and clinical parameters. Relative quantitative (RQ) gene expression was determined using the ∆∆CT method. Results: The mean bleeding on probing values (<i>p</i> = 0.006), clinical attachment loss (CAL) (<i>p</i> = 0.009), gingival recession (<i>p</i> = 0.020), limited mouth opening (<i>p</i> = 0.001) and cervical tooth defects (<i>p</i> = 0.011) were significantly higher in group 1. RQ expressions of IL-2 and CD34 were significantly lower, IL-6, MMP-9, and CD90 were significantly higher. There was a significant positive correlation of IL-6/MMP-9 and negative correlation of mouth opening/CAL and IL-6/CAL. Conclusion: Different expression levels of IL-2, IL-6, MMP-9, CD34 and CD90 were detected in biofilm samples from patients with SSc compared to control. An immunological correlation to the clinical parameters of mouth opening and CAL was shown; thus, we conclude that SSc might have an impact on periodontal tissues.Mayte BuchbenderAmelie LugenbühlJakob FehlhoferChristian KirschneckJutta RiesRainer LutzMichael SticherlingMarco Rainer KestingMDPI AGarticlebiofilm samplesexpression levels of cytokinesperiodontal parameterssystemic scleroderma (SSc)ScienceQENLife, Vol 11, Iss 1145, p 1145 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	biofilm samples expression levels of cytokines periodontal parameters systemic scleroderma (SSc) Science Q
spellingShingle	biofilm samples expression levels of cytokines periodontal parameters systemic scleroderma (SSc) Science Q Mayte Buchbender Amelie Lugenbühl Jakob Fehlhofer Christian Kirschneck Jutta Ries Rainer Lutz Michael Sticherling Marco Rainer Kesting Investigation of the Expression of Inflammatory Markers in Oral Biofilm Samples in Patients with Systemic Scleroderma and the Association with Clinical Periodontal Parameters—A Preliminary Study
description	Background: Systemic scleroderma (SSc) has multiple orofacial effects. The aim of this study was to analyze the expression of inflammatory mediators in biofilm samples. It was hypothesized that different expression levels and clinical associations might be drawn. Methods: A total of 39 biofilm samples from group 1 = SSc and group 2 = healthy control were examined for the expression levels of interleukin (IL)-2,-6, and -10; matrix metalloprotease (MMP)-9; and surface antigens CD90 and CD34 by quantitative real-time PCR and clinical parameters. Relative quantitative (RQ) gene expression was determined using the ∆∆CT method. Results: The mean bleeding on probing values (<i>p</i> = 0.006), clinical attachment loss (CAL) (<i>p</i> = 0.009), gingival recession (<i>p</i> = 0.020), limited mouth opening (<i>p</i> = 0.001) and cervical tooth defects (<i>p</i> = 0.011) were significantly higher in group 1. RQ expressions of IL-2 and CD34 were significantly lower, IL-6, MMP-9, and CD90 were significantly higher. There was a significant positive correlation of IL-6/MMP-9 and negative correlation of mouth opening/CAL and IL-6/CAL. Conclusion: Different expression levels of IL-2, IL-6, MMP-9, CD34 and CD90 were detected in biofilm samples from patients with SSc compared to control. An immunological correlation to the clinical parameters of mouth opening and CAL was shown; thus, we conclude that SSc might have an impact on periodontal tissues.
format	article
author	Mayte Buchbender Amelie Lugenbühl Jakob Fehlhofer Christian Kirschneck Jutta Ries Rainer Lutz Michael Sticherling Marco Rainer Kesting
author_facet	Mayte Buchbender Amelie Lugenbühl Jakob Fehlhofer Christian Kirschneck Jutta Ries Rainer Lutz Michael Sticherling Marco Rainer Kesting
author_sort	Mayte Buchbender
title	Investigation of the Expression of Inflammatory Markers in Oral Biofilm Samples in Patients with Systemic Scleroderma and the Association with Clinical Periodontal Parameters—A Preliminary Study
title_short	Investigation of the Expression of Inflammatory Markers in Oral Biofilm Samples in Patients with Systemic Scleroderma and the Association with Clinical Periodontal Parameters—A Preliminary Study
title_full	Investigation of the Expression of Inflammatory Markers in Oral Biofilm Samples in Patients with Systemic Scleroderma and the Association with Clinical Periodontal Parameters—A Preliminary Study
title_fullStr	Investigation of the Expression of Inflammatory Markers in Oral Biofilm Samples in Patients with Systemic Scleroderma and the Association with Clinical Periodontal Parameters—A Preliminary Study
title_full_unstemmed	Investigation of the Expression of Inflammatory Markers in Oral Biofilm Samples in Patients with Systemic Scleroderma and the Association with Clinical Periodontal Parameters—A Preliminary Study
title_sort	investigation of the expression of inflammatory markers in oral biofilm samples in patients with systemic scleroderma and the association with clinical periodontal parameters—a preliminary study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a6645ad5db5b47a2b8eb3e87222e34f2
work_keys_str_mv	AT maytebuchbender investigationoftheexpressionofinflammatorymarkersinoralbiofilmsamplesinpatientswithsystemicsclerodermaandtheassociationwithclinicalperiodontalparametersapreliminarystudy AT amelielugenbuhl investigationoftheexpressionofinflammatorymarkersinoralbiofilmsamplesinpatientswithsystemicsclerodermaandtheassociationwithclinicalperiodontalparametersapreliminarystudy AT jakobfehlhofer investigationoftheexpressionofinflammatorymarkersinoralbiofilmsamplesinpatientswithsystemicsclerodermaandtheassociationwithclinicalperiodontalparametersapreliminarystudy AT christiankirschneck investigationoftheexpressionofinflammatorymarkersinoralbiofilmsamplesinpatientswithsystemicsclerodermaandtheassociationwithclinicalperiodontalparametersapreliminarystudy AT juttaries investigationoftheexpressionofinflammatorymarkersinoralbiofilmsamplesinpatientswithsystemicsclerodermaandtheassociationwithclinicalperiodontalparametersapreliminarystudy AT rainerlutz investigationoftheexpressionofinflammatorymarkersinoralbiofilmsamplesinpatientswithsystemicsclerodermaandtheassociationwithclinicalperiodontalparametersapreliminarystudy AT michaelsticherling investigationoftheexpressionofinflammatorymarkersinoralbiofilmsamplesinpatientswithsystemicsclerodermaandtheassociationwithclinicalperiodontalparametersapreliminarystudy AT marcorainerkesting investigationoftheexpressionofinflammatorymarkersinoralbiofilmsamplesinpatientswithsystemicsclerodermaandtheassociationwithclinicalperiodontalparametersapreliminarystudy
_version_	1718411516355543040

Investigation of the Expression of Inflammatory Markers in Oral Biofilm Samples in Patients with Systemic Scleroderma and the Association with Clinical Periodontal Parameters—A Preliminary Study

Ejemplares similares