Differential effects of Bartonella henselae on human and feline macro- and micro-vascular endothelial cells.

Bartonella henselae, a zoonotic agent, induces tumors of endothelial cells (ECs), namely bacillary angiomatosis and peliosis in immunosuppressed humans but not in cats. In vitro studies on ECs represent to date the only way to explore the interactions between Bartonella henselae and vascular endothe...

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Autores principales:	Moez Berrich, Claudine Kieda, Catherine Grillon, Martine Monteil, Nathalie Lamerant, Julie Gavard, Henri Jean Boulouis, Nadia Haddad
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a665835a276f4cc183583de9bbe29e2b
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spelling	oai:doaj.org-article:a665835a276f4cc183583de9bbe29e2b2021-11-18T06:53:06ZDifferential effects of Bartonella henselae on human and feline macro- and micro-vascular endothelial cells.1932-620310.1371/journal.pone.0020204https://doaj.org/article/a665835a276f4cc183583de9bbe29e2b2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21637717/?tool=EBIhttps://doaj.org/toc/1932-6203Bartonella henselae, a zoonotic agent, induces tumors of endothelial cells (ECs), namely bacillary angiomatosis and peliosis in immunosuppressed humans but not in cats. In vitro studies on ECs represent to date the only way to explore the interactions between Bartonella henselae and vascular endothelium. However, no comparative study of the interactions between Bartonella henselae and human (incidental host) ECs vs feline (reservoir host) ECs has been carried out because of the absence of any available feline endothelial cell lines.To this purpose, we have developed nine feline EC lines which allowed comparing the effects of Bartonella strains on human and feline micro-vascular ECs representative of the infection development sites such as skin, versus macro-vascular ECs, such as umbilical vein.Our model revealed intrinsic differences between human (Human Skin Microvascular ECs -HSkMEC and Human Umbilical Vein ECs - iHUVEC) and feline ECs susceptibility to Bartonella henselae infection.While no effect was observed on the feline ECs upon Bartonella henselae infection, the human ones displayed accelerated angiogenesis and wound healing.Noticeable differences were demonstrated between human micro- and macro-vasculature derived ECs both in terms of pseudo-tube formation and healing. Interestingly, Bartonella henselae effects on human ECs were also elicited by soluble factors.Neither Bartonella henselae-infected Human Skin Microvascular ECs clinically involved in bacillary angiomatosis, nor feline ECs increased cAMP production, as opposed to HUVEC.Bartonella henselae could stimulate the activation of Vascular Endothelial Growth Factor Receptor-2 (VEGFR-2) in homologous cellular systems and trigger VEGF production by HSkMECs only, but not iHUVEC or any feline ECs tested.These results may explain the decreased pathogenic potential of Bartonella henselae infection for cats as compared to humans and strongly suggest that an autocrine secretion of VEGF by human skin endothelial cells might induce their growth and ultimately lead to bacillary angiomatosis formation.Moez BerrichClaudine KiedaCatherine GrillonMartine MonteilNathalie LamerantJulie GavardJulie GavardHenri Jean BoulouisNadia HaddadPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e20204 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Moez Berrich Claudine Kieda Catherine Grillon Martine Monteil Nathalie Lamerant Julie Gavard Julie Gavard Henri Jean Boulouis Nadia Haddad Differential effects of Bartonella henselae on human and feline macro- and micro-vascular endothelial cells.
description	Bartonella henselae, a zoonotic agent, induces tumors of endothelial cells (ECs), namely bacillary angiomatosis and peliosis in immunosuppressed humans but not in cats. In vitro studies on ECs represent to date the only way to explore the interactions between Bartonella henselae and vascular endothelium. However, no comparative study of the interactions between Bartonella henselae and human (incidental host) ECs vs feline (reservoir host) ECs has been carried out because of the absence of any available feline endothelial cell lines.To this purpose, we have developed nine feline EC lines which allowed comparing the effects of Bartonella strains on human and feline micro-vascular ECs representative of the infection development sites such as skin, versus macro-vascular ECs, such as umbilical vein.Our model revealed intrinsic differences between human (Human Skin Microvascular ECs -HSkMEC and Human Umbilical Vein ECs - iHUVEC) and feline ECs susceptibility to Bartonella henselae infection.While no effect was observed on the feline ECs upon Bartonella henselae infection, the human ones displayed accelerated angiogenesis and wound healing.Noticeable differences were demonstrated between human micro- and macro-vasculature derived ECs both in terms of pseudo-tube formation and healing. Interestingly, Bartonella henselae effects on human ECs were also elicited by soluble factors.Neither Bartonella henselae-infected Human Skin Microvascular ECs clinically involved in bacillary angiomatosis, nor feline ECs increased cAMP production, as opposed to HUVEC.Bartonella henselae could stimulate the activation of Vascular Endothelial Growth Factor Receptor-2 (VEGFR-2) in homologous cellular systems and trigger VEGF production by HSkMECs only, but not iHUVEC or any feline ECs tested.These results may explain the decreased pathogenic potential of Bartonella henselae infection for cats as compared to humans and strongly suggest that an autocrine secretion of VEGF by human skin endothelial cells might induce their growth and ultimately lead to bacillary angiomatosis formation.
format	article
author	Moez Berrich Claudine Kieda Catherine Grillon Martine Monteil Nathalie Lamerant Julie Gavard Julie Gavard Henri Jean Boulouis Nadia Haddad
author_facet	Moez Berrich Claudine Kieda Catherine Grillon Martine Monteil Nathalie Lamerant Julie Gavard Julie Gavard Henri Jean Boulouis Nadia Haddad
author_sort	Moez Berrich
title	Differential effects of Bartonella henselae on human and feline macro- and micro-vascular endothelial cells.
title_short	Differential effects of Bartonella henselae on human and feline macro- and micro-vascular endothelial cells.
title_full	Differential effects of Bartonella henselae on human and feline macro- and micro-vascular endothelial cells.
title_fullStr	Differential effects of Bartonella henselae on human and feline macro- and micro-vascular endothelial cells.
title_full_unstemmed	Differential effects of Bartonella henselae on human and feline macro- and micro-vascular endothelial cells.
title_sort	differential effects of bartonella henselae on human and feline macro- and micro-vascular endothelial cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a665835a276f4cc183583de9bbe29e2b
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Differential effects of Bartonella henselae on human and feline macro- and micro-vascular endothelial cells.

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