The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence

Curtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the...

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Autores principales:	Ivica Dimkić, Vibha Bhardwaj, Valeria Carpentieri-Pipolo, Nemanja Kuzmanović, Giuliano Degrassi
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a66d559b8cbd4bafa7dc1f5268458cb7
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spelling	oai:doaj.org-article:a66d559b8cbd4bafa7dc1f5268458cb72021-11-11T06:44:15ZThe chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence1932-6203https://doaj.org/article/a66d559b8cbd4bafa7dc1f5268458cb72021-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8565777/?tool=EBIhttps://doaj.org/toc/1932-6203Curtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm ≥ 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides. Therefore, Curtobacterium sp. GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.Ivica DimkićVibha BhardwajValeria Carpentieri-PipoloNemanja KuzmanovićGiuliano DegrassiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ivica Dimkić Vibha Bhardwaj Valeria Carpentieri-Pipolo Nemanja Kuzmanović Giuliano Degrassi The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence
description	Curtobacterium sp. GD1 was isolated from leaves of conventionally grown soybean in Brazil. It was noteworthy that among all bacteria previously isolated from the same origin, only Curtobacterium sp. GD1 showed a strong chitinase activity. The enzyme was secreted and its production was induced by the presence of colloidal chitin in the medium. The chitinase was partially purified and characterized: molecular weight was approximately 37 kDa and specific activity 90.8 U/mg. Furthermore, Curtobacterium sp. GD1 genome was sequenced and analyzed. Our isolate formed a phylogenetic cluster with four other Curtobacterium spp. strains, with ANIb/ANIm ≥ 98%, representing a new, still non described Curtobacterium species. The circular genome visualization and comparison of genome sequences of strains forming new cluster indicated that most regions within their genomes were highly conserved. The gene associated with chitinase production was identified and the distribution pattern of glycosyl hydrolases genes was assessed. Also, genes associated with catabolism of structural carbohydrates such as oligosaccharides, mixed polysaccharides, plant and animal polysaccharides, as well as genes or gene clusters associated with resistance to antibiotics, toxic compounds and auxin biosynthesis subsystem products were identified. The abundance of putative glycosyl hydrolases in the genome of Curtobacterium sp. GD1 suggests that it has the tools for the hydrolysis of different polysaccharides. Therefore, Curtobacterium sp. GD1 isolated from soybean might be a bioremediator, biocontrol agent, an elicitor of the plant defense responses or simply degrader.
format	article
author	Ivica Dimkić Vibha Bhardwaj Valeria Carpentieri-Pipolo Nemanja Kuzmanović Giuliano Degrassi
author_facet	Ivica Dimkić Vibha Bhardwaj Valeria Carpentieri-Pipolo Nemanja Kuzmanović Giuliano Degrassi
author_sort	Ivica Dimkić
title	The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence
title_short	The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence
title_full	The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence
title_fullStr	The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence
title_full_unstemmed	The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence
title_sort	chitinolytic activity of the curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/a66d559b8cbd4bafa7dc1f5268458cb7
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The chitinolytic activity of the Curtobacterium sp. isolated from field-grown soybean and analysis of its genome sequence

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