Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations

The quality control of probiotic products is the focus of numerous organizations worldwide. Several studies have highlighted the poor microbiological quality of many commercial probiotic formulations in terms of the identity of the contained microorganisms, viability, and purity, thus precluding the...

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Autores principales:	Diletta Mazzantini, Francesco Celandroni, Marco Calvigioni, Adelaide Panattoni, Roberto Labella, Emilia Ghelardi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	probiotics quality viable cells identification gastrointestinal behavior Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/a6724944e3f74eb083603ace93f1635d
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spelling	oai:doaj.org-article:a6724944e3f74eb083603ace93f1635d2021-11-25T17:35:33ZMicrobiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations10.3390/foods101127812304-8158https://doaj.org/article/a6724944e3f74eb083603ace93f1635d2021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2781https://doaj.org/toc/2304-8158The quality control of probiotic products is the focus of numerous organizations worldwide. Several studies have highlighted the poor microbiological quality of many commercial probiotic formulations in terms of the identity of the contained microorganisms, viability, and purity, thus precluding the expected health benefits and representing a potential health risk for consumers. In this paper, we analyzed the contents of two probiotic formulations, one composed of an encapsulated mixture of lactobacilli and bifidobacteria, and one by a lyophilized yeast. The microorganisms contained in the products were quantified and identified using up-to-date methodologies, such as MALDI-TOF MS and metagenomic analysis. Moreover, as acid and bile tolerance is included among the criteria used to select probiotic microorganisms, in vitro tests were performed to evaluate the behavior of the formulations in conditions mimicking the harsh gastric environment and the intestinal fluids. Our results indicate the high quality of the formulations in terms of the enumeration and identification of the contained organisms, as well as the absence of contaminants. Moreover, both products tolerated the acidic conditions well, with encapsulation providing further protection for the microorganisms. A good tolerance to the simulated artificial intestinal conditions was also evidenced for both preparations.Diletta MazzantiniFrancesco CelandroniMarco CalvigioniAdelaide PanattoniRoberto LabellaEmilia GhelardiMDPI AGarticleprobioticsqualityviable cellsidentificationgastrointestinal behaviorChemical technologyTP1-1185ENFoods, Vol 10, Iss 2781, p 2781 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	probiotics quality viable cells identification gastrointestinal behavior Chemical technology TP1-1185
spellingShingle	probiotics quality viable cells identification gastrointestinal behavior Chemical technology TP1-1185 Diletta Mazzantini Francesco Celandroni Marco Calvigioni Adelaide Panattoni Roberto Labella Emilia Ghelardi Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations
description	The quality control of probiotic products is the focus of numerous organizations worldwide. Several studies have highlighted the poor microbiological quality of many commercial probiotic formulations in terms of the identity of the contained microorganisms, viability, and purity, thus precluding the expected health benefits and representing a potential health risk for consumers. In this paper, we analyzed the contents of two probiotic formulations, one composed of an encapsulated mixture of lactobacilli and bifidobacteria, and one by a lyophilized yeast. The microorganisms contained in the products were quantified and identified using up-to-date methodologies, such as MALDI-TOF MS and metagenomic analysis. Moreover, as acid and bile tolerance is included among the criteria used to select probiotic microorganisms, in vitro tests were performed to evaluate the behavior of the formulations in conditions mimicking the harsh gastric environment and the intestinal fluids. Our results indicate the high quality of the formulations in terms of the enumeration and identification of the contained organisms, as well as the absence of contaminants. Moreover, both products tolerated the acidic conditions well, with encapsulation providing further protection for the microorganisms. A good tolerance to the simulated artificial intestinal conditions was also evidenced for both preparations.
format	article
author	Diletta Mazzantini Francesco Celandroni Marco Calvigioni Adelaide Panattoni Roberto Labella Emilia Ghelardi
author_facet	Diletta Mazzantini Francesco Celandroni Marco Calvigioni Adelaide Panattoni Roberto Labella Emilia Ghelardi
author_sort	Diletta Mazzantini
title	Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations
title_short	Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations
title_full	Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations
title_fullStr	Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations
title_full_unstemmed	Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations
title_sort	microbiological quality and resistance to an artificial gut environment of two probiotic formulations
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a6724944e3f74eb083603ace93f1635d
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Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations

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