Tumorigenic mesenchymal clusters are less sensitive to moderate osmotic stresses due to low amounts of junctional E-cadherin

Abstract The migration of tumorigenic cells is a critical step for metastatic breast cancer progression. Although the role of the extracellular matrix in breast cancer cell migration has been extensively described, the effect of osmotic stress on the migration of tumor breast cohorts remains unclear...

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Autores principales:	Danahe Mohammed, Chan Young Park, Jeffrey J. Fredberg, David A. Weitz
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a67a8a4ddc1e4d82b479eebee690dd40
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spelling	oai:doaj.org-article:a67a8a4ddc1e4d82b479eebee690dd402021-12-02T16:27:45ZTumorigenic mesenchymal clusters are less sensitive to moderate osmotic stresses due to low amounts of junctional E-cadherin10.1038/s41598-021-95740-x2045-2322https://doaj.org/article/a67a8a4ddc1e4d82b479eebee690dd402021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95740-xhttps://doaj.org/toc/2045-2322Abstract The migration of tumorigenic cells is a critical step for metastatic breast cancer progression. Although the role of the extracellular matrix in breast cancer cell migration has been extensively described, the effect of osmotic stress on the migration of tumor breast cohorts remains unclear. Most of our understanding on the effect of osmotic stresses on cell migration comes from studies at the level of the single cell in isolation and does not take cell–cell interactions into account. Here, we study the impact of moderate osmotic stress on the migration of cell clusters composed of either non-tumorigenic or tumorigenic cells. We observe a decrease in migration distance and speed for non-tumorigenic cells but not for tumorigenic ones. To explain these differences, we investigate how osmotic stress impacts the mechanical properties of cell clusters and affects their volumes. Our findings show that tumorigenic mesenchymal cells are less sensitive to osmotic stress than non-tumorigenic cells and suggest that this difference is associated with a lower expression of E-cadherin. Using EGTA treatments, we confirm that the establishment of cell–cell adhesive interactions is a key component of the behavior of cell clusters in response to osmotic stress. This study provides evidence on the low sensitivity of mesenchymal tumorigenic clusters to moderate osmotic stress and highlights the importance of cadherin-based junctions in the response to osmotic stress.Danahe MohammedChan Young ParkJeffrey J. FredbergDavid A. WeitzNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Danahe Mohammed Chan Young Park Jeffrey J. Fredberg David A. Weitz Tumorigenic mesenchymal clusters are less sensitive to moderate osmotic stresses due to low amounts of junctional E-cadherin
description	Abstract The migration of tumorigenic cells is a critical step for metastatic breast cancer progression. Although the role of the extracellular matrix in breast cancer cell migration has been extensively described, the effect of osmotic stress on the migration of tumor breast cohorts remains unclear. Most of our understanding on the effect of osmotic stresses on cell migration comes from studies at the level of the single cell in isolation and does not take cell–cell interactions into account. Here, we study the impact of moderate osmotic stress on the migration of cell clusters composed of either non-tumorigenic or tumorigenic cells. We observe a decrease in migration distance and speed for non-tumorigenic cells but not for tumorigenic ones. To explain these differences, we investigate how osmotic stress impacts the mechanical properties of cell clusters and affects their volumes. Our findings show that tumorigenic mesenchymal cells are less sensitive to osmotic stress than non-tumorigenic cells and suggest that this difference is associated with a lower expression of E-cadherin. Using EGTA treatments, we confirm that the establishment of cell–cell adhesive interactions is a key component of the behavior of cell clusters in response to osmotic stress. This study provides evidence on the low sensitivity of mesenchymal tumorigenic clusters to moderate osmotic stress and highlights the importance of cadherin-based junctions in the response to osmotic stress.
format	article
author	Danahe Mohammed Chan Young Park Jeffrey J. Fredberg David A. Weitz
author_facet	Danahe Mohammed Chan Young Park Jeffrey J. Fredberg David A. Weitz
author_sort	Danahe Mohammed
title	Tumorigenic mesenchymal clusters are less sensitive to moderate osmotic stresses due to low amounts of junctional E-cadherin
title_short	Tumorigenic mesenchymal clusters are less sensitive to moderate osmotic stresses due to low amounts of junctional E-cadherin
title_full	Tumorigenic mesenchymal clusters are less sensitive to moderate osmotic stresses due to low amounts of junctional E-cadherin
title_fullStr	Tumorigenic mesenchymal clusters are less sensitive to moderate osmotic stresses due to low amounts of junctional E-cadherin
title_full_unstemmed	Tumorigenic mesenchymal clusters are less sensitive to moderate osmotic stresses due to low amounts of junctional E-cadherin
title_sort	tumorigenic mesenchymal clusters are less sensitive to moderate osmotic stresses due to low amounts of junctional e-cadherin
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/a67a8a4ddc1e4d82b479eebee690dd40
work_keys_str_mv	AT danahemohammed tumorigenicmesenchymalclustersarelesssensitivetomoderateosmoticstressesduetolowamountsofjunctionalecadherin AT chanyoungpark tumorigenicmesenchymalclustersarelesssensitivetomoderateosmoticstressesduetolowamountsofjunctionalecadherin AT jeffreyjfredberg tumorigenicmesenchymalclustersarelesssensitivetomoderateosmoticstressesduetolowamountsofjunctionalecadherin AT davidaweitz tumorigenicmesenchymalclustersarelesssensitivetomoderateosmoticstressesduetolowamountsofjunctionalecadherin
_version_	1718384009532145664

Tumorigenic mesenchymal clusters are less sensitive to moderate osmotic stresses due to low amounts of junctional E-cadherin

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