Rapid evolution of pandemic noroviruses of the GII.4 lineage.

Over the last fifteen years there have been five pandemics of norovirus (NoV) associated gastroenteritis, and the period of stasis between each pandemic has been progressively shortening. NoV is classified into five genogroups, which can be further classified into 25 or more different human NoV geno...

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Autores principales:	Rowena A Bull, John-Sebastian Eden, William D Rawlinson, Peter A White
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a67bcddc1be44087b16019cd039bc050
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spelling	oai:doaj.org-article:a67bcddc1be44087b16019cd039bc0502021-11-25T05:48:12ZRapid evolution of pandemic noroviruses of the GII.4 lineage.1553-73661553-737410.1371/journal.ppat.1000831https://doaj.org/article/a67bcddc1be44087b16019cd039bc0502010-03-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20360972/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Over the last fifteen years there have been five pandemics of norovirus (NoV) associated gastroenteritis, and the period of stasis between each pandemic has been progressively shortening. NoV is classified into five genogroups, which can be further classified into 25 or more different human NoV genotypes; however, only one, genogroup II genotype 4 (GII.4), is associated with pandemics. Hence, GII.4 viruses have both a higher frequency in the host population and greater epidemiological fitness. The aim of this study was to investigate if the accuracy and rate of replication are contributing to the increased epidemiological fitness of the GII.4 strains. The replication and mutation rates were determined using in vitro RNA dependent RNA polymerase (RdRp) assays, and rates of evolution were determined by bioinformatics. GII.4 strains were compared to the second most reported genotype, recombinant GII.b/GII.3, the rarely detected GII.3 and GII.7 and as a control, hepatitis C virus (HCV). The predominant GII.4 strains had a higher mutation rate and rate of evolution compared to the less frequently detected GII.b, GII.3 and GII.7 strains. Furthermore, the GII.4 lineage had on average a 1.7-fold higher rate of evolution within the capsid sequence and a greater number of non-synonymous changes compared to other NoVs, supporting the theory that it is undergoing antigenic drift at a faster rate. Interestingly, the non-synonymous mutations for all three NoV genotypes were localised to common structural residues in the capsid, indicating that these sites are likely to be under immune selection. This study supports the hypothesis that the ability of the virus to generate genetic diversity is vital for viral fitness.Rowena A BullJohn-Sebastian EdenWilliam D RawlinsonPeter A WhitePublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 6, Iss 3, p e1000831 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Rowena A Bull John-Sebastian Eden William D Rawlinson Peter A White Rapid evolution of pandemic noroviruses of the GII.4 lineage.
description	Over the last fifteen years there have been five pandemics of norovirus (NoV) associated gastroenteritis, and the period of stasis between each pandemic has been progressively shortening. NoV is classified into five genogroups, which can be further classified into 25 or more different human NoV genotypes; however, only one, genogroup II genotype 4 (GII.4), is associated with pandemics. Hence, GII.4 viruses have both a higher frequency in the host population and greater epidemiological fitness. The aim of this study was to investigate if the accuracy and rate of replication are contributing to the increased epidemiological fitness of the GII.4 strains. The replication and mutation rates were determined using in vitro RNA dependent RNA polymerase (RdRp) assays, and rates of evolution were determined by bioinformatics. GII.4 strains were compared to the second most reported genotype, recombinant GII.b/GII.3, the rarely detected GII.3 and GII.7 and as a control, hepatitis C virus (HCV). The predominant GII.4 strains had a higher mutation rate and rate of evolution compared to the less frequently detected GII.b, GII.3 and GII.7 strains. Furthermore, the GII.4 lineage had on average a 1.7-fold higher rate of evolution within the capsid sequence and a greater number of non-synonymous changes compared to other NoVs, supporting the theory that it is undergoing antigenic drift at a faster rate. Interestingly, the non-synonymous mutations for all three NoV genotypes were localised to common structural residues in the capsid, indicating that these sites are likely to be under immune selection. This study supports the hypothesis that the ability of the virus to generate genetic diversity is vital for viral fitness.
format	article
author	Rowena A Bull John-Sebastian Eden William D Rawlinson Peter A White
author_facet	Rowena A Bull John-Sebastian Eden William D Rawlinson Peter A White
author_sort	Rowena A Bull
title	Rapid evolution of pandemic noroviruses of the GII.4 lineage.
title_short	Rapid evolution of pandemic noroviruses of the GII.4 lineage.
title_full	Rapid evolution of pandemic noroviruses of the GII.4 lineage.
title_fullStr	Rapid evolution of pandemic noroviruses of the GII.4 lineage.
title_full_unstemmed	Rapid evolution of pandemic noroviruses of the GII.4 lineage.
title_sort	rapid evolution of pandemic noroviruses of the gii.4 lineage.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/a67bcddc1be44087b16019cd039bc050
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Rapid evolution of pandemic noroviruses of the GII.4 lineage.

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