Influence of Light/Dark Cycles on Body Color, Hepatopancreas Metabolism, and Intestinal Microbiota Homeostasis in Litopenaeus vannamei

In aquatic animals, the light/dark cycle acts as an important biological factor that influences the entire life cycle. Until present, evidence regarding the regulation of physiological metabolic process under different light/dark cycles is limited in Litopenaeus vannamei. In this study, we mainly in...

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Autores principales:	Lefei Jiao, Tianmeng Dai, Xinyue Tao, Jingjing Lu, Qicun Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	light/dark cycles multi-omics techniques microbiota homeostasis circadian biological rhythm crustacean physiological metabolism Science Q General. Including nature conservation, geographical distribution QH1-199.5
Acceso en línea:	https://doaj.org/article/a67dc2cb82944d0d9fcb04c064b16a53
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spelling	oai:doaj.org-article:a67dc2cb82944d0d9fcb04c064b16a532021-12-01T13:38:40ZInfluence of Light/Dark Cycles on Body Color, Hepatopancreas Metabolism, and Intestinal Microbiota Homeostasis in Litopenaeus vannamei2296-774510.3389/fmars.2021.750384https://doaj.org/article/a67dc2cb82944d0d9fcb04c064b16a532021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmars.2021.750384/fullhttps://doaj.org/toc/2296-7745In aquatic animals, the light/dark cycle acts as an important biological factor that influences the entire life cycle. Until present, evidence regarding the regulation of physiological metabolic process under different light/dark cycles is limited in Litopenaeus vannamei. In this study, we mainly investigated the effects of different light/dark cycles (12 h light/12 h dark, 0 h light/24 h dark) on the hepatopancreas metabolism and intestinal microbiota homeostasis in L. vannamei using multiomics techniques. One interesting finding was that the body color of L. vannamei became darker after dark treatment for 8 weeks. Further hepatopancreas transcriptome analysis identified down-regulated genes involved in regulating nutrition metabolism, body-color formation, diurnal rhythm, immune function, hormone levels, and posttranslational modifications. The intestinal microbiota analysis showed that dark treatment-induced alterations in intestinal bacterial abundances in L. vannamei, such as decreased (P < 0.05) relative abundance of Formosa, Demequina, Lutimonas and increased (P < 0.05) relative abundance of Ruegeria, Vibrio, Actibacter, Roseovarius, Ilumatobacter, and Kriegella at the genus level. The microbiota functional analysis demonstrated that the dark treatment mainly increased susceptibility of pathogens, decreased nutrition metabolism, and influenced circadian rhythm. This study indicated for the first time that constant darkness treatment darkened the body color and altered hepatopancreas metabolism and intestinal microbiota homeostasis in L. vannamei, which might give potential clues for improving the productive capacities by changing light/dark cycles in shrimp farming.Lefei JiaoTianmeng DaiXinyue TaoJingjing LuQicun ZhouFrontiers Media S.A.articlelight/dark cyclesmulti-omics techniquesmicrobiota homeostasiscircadian biological rhythmcrustaceanphysiological metabolismScienceQGeneral. Including nature conservation, geographical distributionQH1-199.5ENFrontiers in Marine Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	light/dark cycles multi-omics techniques microbiota homeostasis circadian biological rhythm crustacean physiological metabolism Science Q General. Including nature conservation, geographical distribution QH1-199.5
spellingShingle	light/dark cycles multi-omics techniques microbiota homeostasis circadian biological rhythm crustacean physiological metabolism Science Q General. Including nature conservation, geographical distribution QH1-199.5 Lefei Jiao Tianmeng Dai Xinyue Tao Jingjing Lu Qicun Zhou Influence of Light/Dark Cycles on Body Color, Hepatopancreas Metabolism, and Intestinal Microbiota Homeostasis in Litopenaeus vannamei
description	In aquatic animals, the light/dark cycle acts as an important biological factor that influences the entire life cycle. Until present, evidence regarding the regulation of physiological metabolic process under different light/dark cycles is limited in Litopenaeus vannamei. In this study, we mainly investigated the effects of different light/dark cycles (12 h light/12 h dark, 0 h light/24 h dark) on the hepatopancreas metabolism and intestinal microbiota homeostasis in L. vannamei using multiomics techniques. One interesting finding was that the body color of L. vannamei became darker after dark treatment for 8 weeks. Further hepatopancreas transcriptome analysis identified down-regulated genes involved in regulating nutrition metabolism, body-color formation, diurnal rhythm, immune function, hormone levels, and posttranslational modifications. The intestinal microbiota analysis showed that dark treatment-induced alterations in intestinal bacterial abundances in L. vannamei, such as decreased (P < 0.05) relative abundance of Formosa, Demequina, Lutimonas and increased (P < 0.05) relative abundance of Ruegeria, Vibrio, Actibacter, Roseovarius, Ilumatobacter, and Kriegella at the genus level. The microbiota functional analysis demonstrated that the dark treatment mainly increased susceptibility of pathogens, decreased nutrition metabolism, and influenced circadian rhythm. This study indicated for the first time that constant darkness treatment darkened the body color and altered hepatopancreas metabolism and intestinal microbiota homeostasis in L. vannamei, which might give potential clues for improving the productive capacities by changing light/dark cycles in shrimp farming.
format	article
author	Lefei Jiao Tianmeng Dai Xinyue Tao Jingjing Lu Qicun Zhou
author_facet	Lefei Jiao Tianmeng Dai Xinyue Tao Jingjing Lu Qicun Zhou
author_sort	Lefei Jiao
title	Influence of Light/Dark Cycles on Body Color, Hepatopancreas Metabolism, and Intestinal Microbiota Homeostasis in Litopenaeus vannamei
title_short	Influence of Light/Dark Cycles on Body Color, Hepatopancreas Metabolism, and Intestinal Microbiota Homeostasis in Litopenaeus vannamei
title_full	Influence of Light/Dark Cycles on Body Color, Hepatopancreas Metabolism, and Intestinal Microbiota Homeostasis in Litopenaeus vannamei
title_fullStr	Influence of Light/Dark Cycles on Body Color, Hepatopancreas Metabolism, and Intestinal Microbiota Homeostasis in Litopenaeus vannamei
title_full_unstemmed	Influence of Light/Dark Cycles on Body Color, Hepatopancreas Metabolism, and Intestinal Microbiota Homeostasis in Litopenaeus vannamei
title_sort	influence of light/dark cycles on body color, hepatopancreas metabolism, and intestinal microbiota homeostasis in litopenaeus vannamei
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a67dc2cb82944d0d9fcb04c064b16a53
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Influence of Light/Dark Cycles on Body Color, Hepatopancreas Metabolism, and Intestinal Microbiota Homeostasis in Litopenaeus vannamei

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