Microscopic optical projection tomography in vivo.

We describe a versatile optical projection tomography system for rapid three-dimensional imaging of microscopic specimens in vivo. Our tomographic setup eliminates the in xy and z strongly asymmetric resolution, resulting from optical sectioning in conventional confocal microscopy. It allows for rob...

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Autores principales:	Matthias Rieckher, Udo Jochen Birk, Heiko Meyer, Jorge Ripoll, Nektarios Tavernarakis
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a67dd433d5f946e78f17410f1567a176
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spelling	oai:doaj.org-article:a67dd433d5f946e78f17410f1567a1762021-11-18T06:54:41ZMicroscopic optical projection tomography in vivo.1932-620310.1371/journal.pone.0018963https://doaj.org/article/a67dd433d5f946e78f17410f1567a1762011-04-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21559481/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203We describe a versatile optical projection tomography system for rapid three-dimensional imaging of microscopic specimens in vivo. Our tomographic setup eliminates the in xy and z strongly asymmetric resolution, resulting from optical sectioning in conventional confocal microscopy. It allows for robust, high resolution fluorescence as well as absorption imaging of live transparent invertebrate animals such as C. elegans. This system offers considerable advantages over currently available methods when imaging dynamic developmental processes and animal ageing; it permits monitoring of spatio-temporal gene expression and anatomical alterations with single-cell resolution, it utilizes both fluorescence and absorption as a source of contrast, and is easily adaptable for a range of small model organisms.Matthias RieckherUdo Jochen BirkHeiko MeyerJorge RipollNektarios TavernarakisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 4, p e18963 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Matthias Rieckher Udo Jochen Birk Heiko Meyer Jorge Ripoll Nektarios Tavernarakis Microscopic optical projection tomography in vivo.
description	We describe a versatile optical projection tomography system for rapid three-dimensional imaging of microscopic specimens in vivo. Our tomographic setup eliminates the in xy and z strongly asymmetric resolution, resulting from optical sectioning in conventional confocal microscopy. It allows for robust, high resolution fluorescence as well as absorption imaging of live transparent invertebrate animals such as C. elegans. This system offers considerable advantages over currently available methods when imaging dynamic developmental processes and animal ageing; it permits monitoring of spatio-temporal gene expression and anatomical alterations with single-cell resolution, it utilizes both fluorescence and absorption as a source of contrast, and is easily adaptable for a range of small model organisms.
format	article
author	Matthias Rieckher Udo Jochen Birk Heiko Meyer Jorge Ripoll Nektarios Tavernarakis
author_facet	Matthias Rieckher Udo Jochen Birk Heiko Meyer Jorge Ripoll Nektarios Tavernarakis
author_sort	Matthias Rieckher
title	Microscopic optical projection tomography in vivo.
title_short	Microscopic optical projection tomography in vivo.
title_full	Microscopic optical projection tomography in vivo.
title_fullStr	Microscopic optical projection tomography in vivo.
title_full_unstemmed	Microscopic optical projection tomography in vivo.
title_sort	microscopic optical projection tomography in vivo.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a67dd433d5f946e78f17410f1567a176
work_keys_str_mv	AT matthiasrieckher microscopicopticalprojectiontomographyinvivo AT udojochenbirk microscopicopticalprojectiontomographyinvivo AT heikomeyer microscopicopticalprojectiontomographyinvivo AT jorgeripoll microscopicopticalprojectiontomographyinvivo AT nektariostavernarakis microscopicopticalprojectiontomographyinvivo
_version_	1718424246010511360

Microscopic optical projection tomography in vivo.

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