Antimicrobial and osteogenic effect of Ag-implanted titanium with a nanostructured surface

Yanhua Zheng1, Jinbo Li2, Xuanyong Liu2, Jiao Sun11Shanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People&rsquo;s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, People&rsquo;s Republic of China; 2State Key...

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Autores principales:	Zheng Y, Li J, Liu X,&nbsp;Sun J
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/a6800611f6a04eed8a553fbe5db7f7ae
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spelling	oai:doaj.org-article:a6800611f6a04eed8a553fbe5db7f7ae2021-12-02T02:49:28ZAntimicrobial and osteogenic effect of Ag-implanted titanium with a nanostructured surface1176-91141178-2013https://doaj.org/article/a6800611f6a04eed8a553fbe5db7f7ae2012-02-01T00:00:00Zhttp://www.dovepress.com/antimicrobial-and-osteogenic-effect-of-ag-implanted-titanium-with-a-na-a9313https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Yanhua Zheng1, Jinbo Li2, Xuanyong Liu2, Jiao Sun11Shanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People&rsquo;s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, People&rsquo;s Republic of China; 2State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, People&rsquo;s Republic of ChinaAbstract: Ag-implanted titanium with a nanostructured surface was prepared by hydrothermal treatment with H2O2 followed by Ag plasma immersion ion implantation. Streptococcus mutans, Porphyromonas gingivalis and Candida albicans were chosen for antimicrobial tests. Genes related to microbial structure or adhesion, namely glucan-binding proteins B (GbpB), fimbria protein A (FimA), and agglutinin-like sequence4 (Als4), were examined. The osteoblast&rsquo;s attachment, viability, and quantitative analysis of osteogenic gene expression (Alp, Ocn, RunX2) on titanium surfaces were evaluated. Scanning electron microscopy (SEM) revealed that Ag nanoparticles of approximately 10 nm were incorporated on the nanostructured surface of titanium after Ag plasma immersion ion implantation. Trials showed that 93.99% of S. mutans, 93.57% of P. g, and 89.78% of C. albicans were killed on the Ag-implanted titanium with a nanostructured surface. Gene expressions from the three microorganisms confirmed the antimicrobial activities of the Ag-implanted titanium with a nanostructured surface. Furthermore, the adhesive images and viability assays indicated that the Ag-implanted titanium with a nanostructured surface did not impair osteoblasts. The expressions of osteoblast phenotype genes in cells grown on the Ag-implanted titanium surface were significantly increased. The results of this study suggest that the Ag-implanted titanium with a nanostructured surface displays good antimicrobial properties, reducing gene expressions of microorganisms, and excellent cell adhesion and osteogenic effects.Keywords: titanium, nanostructured, silver, antimicrobial, osteogenicZheng YLi JLiu X,&nbsp;Sun JDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 875-884 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zheng Y Li J Liu X,&nbsp;Sun J Antimicrobial and osteogenic effect of Ag-implanted titanium with a nanostructured surface
description	Yanhua Zheng1, Jinbo Li2, Xuanyong Liu2, Jiao Sun11Shanghai Biomaterials Research and Testing Center, Shanghai Key Laboratory of Stomatology, Ninth People&rsquo;s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, People&rsquo;s Republic of China; 2State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai 200050, People&rsquo;s Republic of ChinaAbstract: Ag-implanted titanium with a nanostructured surface was prepared by hydrothermal treatment with H2O2 followed by Ag plasma immersion ion implantation. Streptococcus mutans, Porphyromonas gingivalis and Candida albicans were chosen for antimicrobial tests. Genes related to microbial structure or adhesion, namely glucan-binding proteins B (GbpB), fimbria protein A (FimA), and agglutinin-like sequence4 (Als4), were examined. The osteoblast&rsquo;s attachment, viability, and quantitative analysis of osteogenic gene expression (Alp, Ocn, RunX2) on titanium surfaces were evaluated. Scanning electron microscopy (SEM) revealed that Ag nanoparticles of approximately 10 nm were incorporated on the nanostructured surface of titanium after Ag plasma immersion ion implantation. Trials showed that 93.99% of S. mutans, 93.57% of P. g, and 89.78% of C. albicans were killed on the Ag-implanted titanium with a nanostructured surface. Gene expressions from the three microorganisms confirmed the antimicrobial activities of the Ag-implanted titanium with a nanostructured surface. Furthermore, the adhesive images and viability assays indicated that the Ag-implanted titanium with a nanostructured surface did not impair osteoblasts. The expressions of osteoblast phenotype genes in cells grown on the Ag-implanted titanium surface were significantly increased. The results of this study suggest that the Ag-implanted titanium with a nanostructured surface displays good antimicrobial properties, reducing gene expressions of microorganisms, and excellent cell adhesion and osteogenic effects.Keywords: titanium, nanostructured, silver, antimicrobial, osteogenic
format	article
author	Zheng Y Li J Liu X,&nbsp;Sun J
author_facet	Zheng Y Li J Liu X,&nbsp;Sun J
author_sort	Zheng Y
title	Antimicrobial and osteogenic effect of Ag-implanted titanium with a nanostructured surface
title_short	Antimicrobial and osteogenic effect of Ag-implanted titanium with a nanostructured surface
title_full	Antimicrobial and osteogenic effect of Ag-implanted titanium with a nanostructured surface
title_fullStr	Antimicrobial and osteogenic effect of Ag-implanted titanium with a nanostructured surface
title_full_unstemmed	Antimicrobial and osteogenic effect of Ag-implanted titanium with a nanostructured surface
title_sort	antimicrobial and osteogenic effect of ag-implanted titanium with a nanostructured surface
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/a6800611f6a04eed8a553fbe5db7f7ae
work_keys_str_mv	AT zhengy antimicrobialandosteogeniceffectofagimplantedtitaniumwithananostructuredsurface AT lij antimicrobialandosteogeniceffectofagimplantedtitaniumwithananostructuredsurface AT liuxampnbspsunj antimicrobialandosteogeniceffectofagimplantedtitaniumwithananostructuredsurface
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Antimicrobial and osteogenic effect of Ag-implanted titanium with a nanostructured surface

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