X-Ray Phase Contrast Tomography Reveals Early Vascular Alterations and Neuronal Loss in a Multiple Sclerosis Model

Abstract The degenerative effects of multiple sclerosis at the level of the vascular and neuronal networks in the central nervous system are currently the object of intensive investigation. Preclinical studies have demonstrated the efficacy of mesenchymal stem cell (MSC) therapy in experimental auto...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	A. Cedola, A. Bravin, I. Bukreeva, M. Fratini, A. Pacureanu, A. Mittone, L. Massimi, P. Cloetens, P. Coan, G. Campi, R. Spanò, F. Brun, V. Grigoryev, V. Petrosino, C. Venturi, M. Mastrogiacomo, Nicole Kerlero de Rosbo, A. Uccelli
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6849489a53a4ad5b13dfa2935d58991
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a6849489a53a4ad5b13dfa2935d58991
record_format	dspace
spelling	oai:doaj.org-article:a6849489a53a4ad5b13dfa2935d589912021-12-02T11:41:22ZX-Ray Phase Contrast Tomography Reveals Early Vascular Alterations and Neuronal Loss in a Multiple Sclerosis Model10.1038/s41598-017-06251-72045-2322https://doaj.org/article/a6849489a53a4ad5b13dfa2935d589912017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06251-7https://doaj.org/toc/2045-2322Abstract The degenerative effects of multiple sclerosis at the level of the vascular and neuronal networks in the central nervous system are currently the object of intensive investigation. Preclinical studies have demonstrated the efficacy of mesenchymal stem cell (MSC) therapy in experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis, but the neuropathology of specific lesions in EAE and the effects of MSC treatment are under debate. Because conventional imaging techniques entail protocols that alter the tissues, limiting the reliability of the results, we have used non-invasive X-ray phase-contrast tomography to obtain an unprecedented direct 3D characterization of EAE lesions at micro-to-nano scales, with simultaneous imaging of the vascular and neuronal networks. We reveal EAE-mediated alterations down to the capillary network. Our findings shed light on how the disease and MSC treatment affect the tissues, and promote X-ray phase-contrast tomography as a powerful tool for studying neurovascular diseases and monitoring advanced therapies.A. CedolaA. BravinI. BukreevaM. FratiniA. PacureanuA. MittoneL. MassimiP. CloetensP. CoanG. CampiR. SpanòF. BrunV. GrigoryevV. PetrosinoC. VenturiM. MastrogiacomoNicole Kerlero de RosboA. UccelliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q A. Cedola A. Bravin I. Bukreeva M. Fratini A. Pacureanu A. Mittone L. Massimi P. Cloetens P. Coan G. Campi R. Spanò F. Brun V. Grigoryev V. Petrosino C. Venturi M. Mastrogiacomo Nicole Kerlero de Rosbo A. Uccelli X-Ray Phase Contrast Tomography Reveals Early Vascular Alterations and Neuronal Loss in a Multiple Sclerosis Model
description	Abstract The degenerative effects of multiple sclerosis at the level of the vascular and neuronal networks in the central nervous system are currently the object of intensive investigation. Preclinical studies have demonstrated the efficacy of mesenchymal stem cell (MSC) therapy in experimental autoimmune encephalomyelitis (EAE), the animal model for multiple sclerosis, but the neuropathology of specific lesions in EAE and the effects of MSC treatment are under debate. Because conventional imaging techniques entail protocols that alter the tissues, limiting the reliability of the results, we have used non-invasive X-ray phase-contrast tomography to obtain an unprecedented direct 3D characterization of EAE lesions at micro-to-nano scales, with simultaneous imaging of the vascular and neuronal networks. We reveal EAE-mediated alterations down to the capillary network. Our findings shed light on how the disease and MSC treatment affect the tissues, and promote X-ray phase-contrast tomography as a powerful tool for studying neurovascular diseases and monitoring advanced therapies.
format	article
author	A. Cedola A. Bravin I. Bukreeva M. Fratini A. Pacureanu A. Mittone L. Massimi P. Cloetens P. Coan G. Campi R. Spanò F. Brun V. Grigoryev V. Petrosino C. Venturi M. Mastrogiacomo Nicole Kerlero de Rosbo A. Uccelli
author_facet	A. Cedola A. Bravin I. Bukreeva M. Fratini A. Pacureanu A. Mittone L. Massimi P. Cloetens P. Coan G. Campi R. Spanò F. Brun V. Grigoryev V. Petrosino C. Venturi M. Mastrogiacomo Nicole Kerlero de Rosbo A. Uccelli
author_sort	A. Cedola
title	X-Ray Phase Contrast Tomography Reveals Early Vascular Alterations and Neuronal Loss in a Multiple Sclerosis Model
title_short	X-Ray Phase Contrast Tomography Reveals Early Vascular Alterations and Neuronal Loss in a Multiple Sclerosis Model
title_full	X-Ray Phase Contrast Tomography Reveals Early Vascular Alterations and Neuronal Loss in a Multiple Sclerosis Model
title_fullStr	X-Ray Phase Contrast Tomography Reveals Early Vascular Alterations and Neuronal Loss in a Multiple Sclerosis Model
title_full_unstemmed	X-Ray Phase Contrast Tomography Reveals Early Vascular Alterations and Neuronal Loss in a Multiple Sclerosis Model
title_sort	x-ray phase contrast tomography reveals early vascular alterations and neuronal loss in a multiple sclerosis model
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/a6849489a53a4ad5b13dfa2935d58991
work_keys_str_mv	AT acedola xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT abravin xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT ibukreeva xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT mfratini xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT apacureanu xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT amittone xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT lmassimi xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT pcloetens xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT pcoan xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT gcampi xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT rspano xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT fbrun xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT vgrigoryev xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT vpetrosino xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT cventuri xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT mmastrogiacomo xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT nicolekerleroderosbo xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel AT auccelli xrayphasecontrasttomographyrevealsearlyvascularalterationsandneuronallossinamultiplesclerosismodel
_version_	1718395416846794752

X-Ray Phase Contrast Tomography Reveals Early Vascular Alterations and Neuronal Loss in a Multiple Sclerosis Model

Ejemplares similares