Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation

Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and...

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Autores principales: Mitchell Henry Wright, Joseph Adelskov, Anthony Carlson Greene
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Lenguaje:EN
Publicado: American Society for Microbiology 2017
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spelling oai:doaj.org-article:a6851cb1717a49bdbc17247e08bab6792021-11-15T15:04:07ZBacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation10.1128/jmbe.v18i2.13481935-78851935-7877https://doaj.org/article/a6851cb1717a49bdbc17247e08bab6792017-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v18i2.1348https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and discuss how this can be incorporated into microbiology laboratory courses for biology majors.Mitchell Henry WrightJoseph AdelskovAnthony Carlson GreeneAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 18, Iss 2 (2017)
institution DOAJ
collection DOAJ
language EN
topic Special aspects of education
LC8-6691
Biology (General)
QH301-705.5
spellingShingle Special aspects of education
LC8-6691
Biology (General)
QH301-705.5
Mitchell Henry Wright
Joseph Adelskov
Anthony Carlson Greene
Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation
description Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and discuss how this can be incorporated into microbiology laboratory courses for biology majors.
format article
author Mitchell Henry Wright
Joseph Adelskov
Anthony Carlson Greene
author_facet Mitchell Henry Wright
Joseph Adelskov
Anthony Carlson Greene
author_sort Mitchell Henry Wright
title Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation
title_short Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation
title_full Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation
title_fullStr Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation
title_full_unstemmed Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation
title_sort bacterial dna extraction using individual enzymes and phenol/chloroform separation
publisher American Society for Microbiology
publishDate 2017
url https://doaj.org/article/a6851cb1717a49bdbc17247e08bab679
work_keys_str_mv AT mitchellhenrywright bacterialdnaextractionusingindividualenzymesandphenolchloroformseparation
AT josephadelskov bacterialdnaextractionusingindividualenzymesandphenolchloroformseparation
AT anthonycarlsongreene bacterialdnaextractionusingindividualenzymesandphenolchloroformseparation
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