Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation

Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and...

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Auteurs principaux:	Mitchell Henry Wright, Joseph Adelskov, Anthony Carlson Greene
Format:	article
Langue:	EN
Publié:	American Society for Microbiology 2017
Sujets:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Accès en ligne:	https://doaj.org/article/a6851cb1717a49bdbc17247e08bab679
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spelling	oai:doaj.org-article:a6851cb1717a49bdbc17247e08bab6792021-11-15T15:04:07ZBacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation10.1128/jmbe.v18i2.13481935-78851935-7877https://doaj.org/article/a6851cb1717a49bdbc17247e08bab6792017-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v18i2.1348https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and discuss how this can be incorporated into microbiology laboratory courses for biology majors.Mitchell Henry WrightJoseph AdelskovAnthony Carlson GreeneAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 18, Iss 2 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Mitchell Henry Wright Joseph Adelskov Anthony Carlson Greene Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation
description	Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and discuss how this can be incorporated into microbiology laboratory courses for biology majors.
format	article
author	Mitchell Henry Wright Joseph Adelskov Anthony Carlson Greene
author_facet	Mitchell Henry Wright Joseph Adelskov Anthony Carlson Greene
author_sort	Mitchell Henry Wright
title	Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation
title_short	Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation
title_full	Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation
title_fullStr	Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation
title_full_unstemmed	Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation
title_sort	bacterial dna extraction using individual enzymes and phenol/chloroform separation
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/a6851cb1717a49bdbc17247e08bab679
work_keys_str_mv	AT mitchellhenrywright bacterialdnaextractionusingindividualenzymesandphenolchloroformseparation AT josephadelskov bacterialdnaextractionusingindividualenzymesandphenolchloroformseparation AT anthonycarlsongreene bacterialdnaextractionusingindividualenzymesandphenolchloroformseparation
_version_	1718428266464804864

Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation

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