Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation
Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and...
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American Society for Microbiology
2017
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oai:doaj.org-article:a6851cb1717a49bdbc17247e08bab6792021-11-15T15:04:07ZBacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation10.1128/jmbe.v18i2.13481935-78851935-7877https://doaj.org/article/a6851cb1717a49bdbc17247e08bab6792017-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v18i2.1348https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and discuss how this can be incorporated into microbiology laboratory courses for biology majors.Mitchell Henry WrightJoseph AdelskovAnthony Carlson GreeneAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 18, Iss 2 (2017) |
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Special aspects of education LC8-6691 Biology (General) QH301-705.5 |
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Special aspects of education LC8-6691 Biology (General) QH301-705.5 Mitchell Henry Wright Joseph Adelskov Anthony Carlson Greene Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation |
description |
Marmur (4) developed one of the first detailed comprehensive methods for purifying bacterial DNA. This procedure is now outdated, and can be difficult to follow for those with limited experience in molecular biology. Here, we provide a modernized, simplified protocol for extracting bacterial DNA and discuss how this can be incorporated into microbiology laboratory courses for biology majors. |
format |
article |
author |
Mitchell Henry Wright Joseph Adelskov Anthony Carlson Greene |
author_facet |
Mitchell Henry Wright Joseph Adelskov Anthony Carlson Greene |
author_sort |
Mitchell Henry Wright |
title |
Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation |
title_short |
Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation |
title_full |
Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation |
title_fullStr |
Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation |
title_full_unstemmed |
Bacterial DNA Extraction Using Individual Enzymes and Phenol/Chloroform Separation |
title_sort |
bacterial dna extraction using individual enzymes and phenol/chloroform separation |
publisher |
American Society for Microbiology |
publishDate |
2017 |
url |
https://doaj.org/article/a6851cb1717a49bdbc17247e08bab679 |
work_keys_str_mv |
AT mitchellhenrywright bacterialdnaextractionusingindividualenzymesandphenolchloroformseparation AT josephadelskov bacterialdnaextractionusingindividualenzymesandphenolchloroformseparation AT anthonycarlsongreene bacterialdnaextractionusingindividualenzymesandphenolchloroformseparation |
_version_ |
1718428266464804864 |