Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics

Tsai, Zhang, Scholten et al. develop a surfactant- assisted one-pot sample preparation coupled with mass spectrometry method (SOP-MS) for label-free global single-cell proteomics. This method allows researchers to measure hundreds of proteins from single human cells, suggesting its utility for quant...

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Autores principales: Chia-Feng Tsai, Pengfei Zhang, David Scholten, Kendall Martin, Yi-Ting Wang, Rui Zhao, William B. Chrisler, Dhwani B. Patel, Maowei Dou, Yuzhi Jia, Carolina Reduzzi, Xia Liu, Ronald J. Moore, Kristin E. Burnum-Johnson, Miao-Hsia Lin, Chuan-Chih Hsu, Jon M. Jacobs, Jacob Kagan, Sudhir Srivastava, Karin D. Rodland, H. Steven Wiley, Wei-Jun Qian, Richard D. Smith, Ying Zhu, Massimo Cristofanilli, Tao Liu, Huiping Liu, Tujin Shi
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Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/a689739f342a49b381b96ffab619ff1e
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spelling oai:doaj.org-article:a689739f342a49b381b96ffab619ff1e2021-12-02T13:34:31ZSurfactant-assisted one-pot sample preparation for label-free single-cell proteomics10.1038/s42003-021-01797-92399-3642https://doaj.org/article/a689739f342a49b381b96ffab619ff1e2021-03-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-01797-9https://doaj.org/toc/2399-3642Tsai, Zhang, Scholten et al. develop a surfactant- assisted one-pot sample preparation coupled with mass spectrometry method (SOP-MS) for label-free global single-cell proteomics. This method allows researchers to measure hundreds of proteins from single human cells, suggesting its utility for quantitative single-cell proteomics.Chia-Feng TsaiPengfei ZhangDavid ScholtenKendall MartinYi-Ting WangRui ZhaoWilliam B. ChrislerDhwani B. PatelMaowei DouYuzhi JiaCarolina ReduzziXia LiuRonald J. MooreKristin E. Burnum-JohnsonMiao-Hsia LinChuan-Chih HsuJon M. JacobsJacob KaganSudhir SrivastavaKarin D. RodlandH. Steven WileyWei-Jun QianRichard D. SmithYing ZhuMassimo CristofanilliTao LiuHuiping LiuTujin ShiNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-12 (2021)
institution DOAJ
collection DOAJ
language EN
topic Biology (General)
QH301-705.5
spellingShingle Biology (General)
QH301-705.5
Chia-Feng Tsai
Pengfei Zhang
David Scholten
Kendall Martin
Yi-Ting Wang
Rui Zhao
William B. Chrisler
Dhwani B. Patel
Maowei Dou
Yuzhi Jia
Carolina Reduzzi
Xia Liu
Ronald J. Moore
Kristin E. Burnum-Johnson
Miao-Hsia Lin
Chuan-Chih Hsu
Jon M. Jacobs
Jacob Kagan
Sudhir Srivastava
Karin D. Rodland
H. Steven Wiley
Wei-Jun Qian
Richard D. Smith
Ying Zhu
Massimo Cristofanilli
Tao Liu
Huiping Liu
Tujin Shi
Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics
description Tsai, Zhang, Scholten et al. develop a surfactant- assisted one-pot sample preparation coupled with mass spectrometry method (SOP-MS) for label-free global single-cell proteomics. This method allows researchers to measure hundreds of proteins from single human cells, suggesting its utility for quantitative single-cell proteomics.
format article
author Chia-Feng Tsai
Pengfei Zhang
David Scholten
Kendall Martin
Yi-Ting Wang
Rui Zhao
William B. Chrisler
Dhwani B. Patel
Maowei Dou
Yuzhi Jia
Carolina Reduzzi
Xia Liu
Ronald J. Moore
Kristin E. Burnum-Johnson
Miao-Hsia Lin
Chuan-Chih Hsu
Jon M. Jacobs
Jacob Kagan
Sudhir Srivastava
Karin D. Rodland
H. Steven Wiley
Wei-Jun Qian
Richard D. Smith
Ying Zhu
Massimo Cristofanilli
Tao Liu
Huiping Liu
Tujin Shi
author_facet Chia-Feng Tsai
Pengfei Zhang
David Scholten
Kendall Martin
Yi-Ting Wang
Rui Zhao
William B. Chrisler
Dhwani B. Patel
Maowei Dou
Yuzhi Jia
Carolina Reduzzi
Xia Liu
Ronald J. Moore
Kristin E. Burnum-Johnson
Miao-Hsia Lin
Chuan-Chih Hsu
Jon M. Jacobs
Jacob Kagan
Sudhir Srivastava
Karin D. Rodland
H. Steven Wiley
Wei-Jun Qian
Richard D. Smith
Ying Zhu
Massimo Cristofanilli
Tao Liu
Huiping Liu
Tujin Shi
author_sort Chia-Feng Tsai
title Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics
title_short Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics
title_full Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics
title_fullStr Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics
title_full_unstemmed Surfactant-assisted one-pot sample preparation for label-free single-cell proteomics
title_sort surfactant-assisted one-pot sample preparation for label-free single-cell proteomics
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/a689739f342a49b381b96ffab619ff1e
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