Transcriptome analysis of male and female Sebastiscus marmoratus.

<h4>Background</h4>The rockfish Sebastiscus marmoratus, which is widely distributed in the East Sea and the South Sea of China, is a sensitive model for the toxic effects and mechanisms of marine contaminants. To gain a global view of the molecular mechanism(s) whereby gene expression ma...

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Autores principales:	Lingbin Sun, Chonggang Wang, Lixing Huang, Meifang Wu, Zhenghong Zuo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6897e75e03a490f868ffe477ae38d53
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spelling	oai:doaj.org-article:a6897e75e03a490f868ffe477ae38d532021-11-18T08:07:21ZTranscriptome analysis of male and female Sebastiscus marmoratus.1932-620310.1371/journal.pone.0050676https://doaj.org/article/a6897e75e03a490f868ffe477ae38d532012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23209808/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The rockfish Sebastiscus marmoratus, which is widely distributed in the East Sea and the South Sea of China, is a sensitive model for the toxic effects and mechanisms of marine contaminants. To gain a global view of the molecular mechanism(s) whereby gene expression may influence sexual dimorphism in S. marmoratus, and to develop a database for further toxicological studies, we performed a large-scale transcriptome study.<h4>Methodology/principal findings</h4>The Illumina DNA sequencing platform was employed to obtain 27,559,578 and 25,821,126 reads from two cDNA libraries generated from adult male and female S. marmoratus, respectively. Transcriptome de novo assembly was carried out with the short reads assembling program-SOAPdenovo. The reads assembled into 78,675 unigenes, of which 38,677 showed homology to existing protein sequences. Clusters of orthologous groups, gene orthology, and the Kyoto Encyclopedia of Genes and Genomes annotations were performed to better understand the functions of these unigenes. There were 1,209 potential sex differentially expressed unigenes, with 1,049 predicted to be differentially expressed in females and 160 in males. Fifteen randomly chosen unigenes were confirmed using real-time PCR as female or male predominantly expressed genes.<h4>Conclusions/significance</h4>This is the first report of an annotated transcriptome of S. marmoratus and identification of sex differentially expressed genes. These data will be of interest to researchers using this model. This work also provides an archive for future studies in molecular mechanisms of sexual dimorphism and evolution and can be used in comparative studies of other fish.Lingbin SunChonggang WangLixing HuangMeifang WuZhenghong ZuoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 11, p e50676 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lingbin Sun Chonggang Wang Lixing Huang Meifang Wu Zhenghong Zuo Transcriptome analysis of male and female Sebastiscus marmoratus.
description	<h4>Background</h4>The rockfish Sebastiscus marmoratus, which is widely distributed in the East Sea and the South Sea of China, is a sensitive model for the toxic effects and mechanisms of marine contaminants. To gain a global view of the molecular mechanism(s) whereby gene expression may influence sexual dimorphism in S. marmoratus, and to develop a database for further toxicological studies, we performed a large-scale transcriptome study.<h4>Methodology/principal findings</h4>The Illumina DNA sequencing platform was employed to obtain 27,559,578 and 25,821,126 reads from two cDNA libraries generated from adult male and female S. marmoratus, respectively. Transcriptome de novo assembly was carried out with the short reads assembling program-SOAPdenovo. The reads assembled into 78,675 unigenes, of which 38,677 showed homology to existing protein sequences. Clusters of orthologous groups, gene orthology, and the Kyoto Encyclopedia of Genes and Genomes annotations were performed to better understand the functions of these unigenes. There were 1,209 potential sex differentially expressed unigenes, with 1,049 predicted to be differentially expressed in females and 160 in males. Fifteen randomly chosen unigenes were confirmed using real-time PCR as female or male predominantly expressed genes.<h4>Conclusions/significance</h4>This is the first report of an annotated transcriptome of S. marmoratus and identification of sex differentially expressed genes. These data will be of interest to researchers using this model. This work also provides an archive for future studies in molecular mechanisms of sexual dimorphism and evolution and can be used in comparative studies of other fish.
format	article
author	Lingbin Sun Chonggang Wang Lixing Huang Meifang Wu Zhenghong Zuo
author_facet	Lingbin Sun Chonggang Wang Lixing Huang Meifang Wu Zhenghong Zuo
author_sort	Lingbin Sun
title	Transcriptome analysis of male and female Sebastiscus marmoratus.
title_short	Transcriptome analysis of male and female Sebastiscus marmoratus.
title_full	Transcriptome analysis of male and female Sebastiscus marmoratus.
title_fullStr	Transcriptome analysis of male and female Sebastiscus marmoratus.
title_full_unstemmed	Transcriptome analysis of male and female Sebastiscus marmoratus.
title_sort	transcriptome analysis of male and female sebastiscus marmoratus.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a6897e75e03a490f868ffe477ae38d53
work_keys_str_mv	AT lingbinsun transcriptomeanalysisofmaleandfemalesebastiscusmarmoratus AT chonggangwang transcriptomeanalysisofmaleandfemalesebastiscusmarmoratus AT lixinghuang transcriptomeanalysisofmaleandfemalesebastiscusmarmoratus AT meifangwu transcriptomeanalysisofmaleandfemalesebastiscusmarmoratus AT zhenghongzuo transcriptomeanalysisofmaleandfemalesebastiscusmarmoratus
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Transcriptome analysis of male and female Sebastiscus marmoratus.

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