Cell-Free Supernatant of Odoribacter splanchnicus Isolated From Human Feces Exhibits Anti-colorectal Cancer Activity

The gut microbiota (GM) has been shown to be closely associated with the development of colorectal cancer (CRC). However, the involvement of GM is CRC has mainly been demonstrated by metagenomic profiling studies showing the compositional difference between the GM of healthy individuals and that of...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Byeong Seob Oh, Won Jung Choi, Ji-Sun Kim, Seoung Woo Ryu, Seung Yeob Yu, Jung-Sook Lee, Seung-Hwan Park, Se Won Kang, Jiyoung Lee, Won Yong Jung, Young-Min Kim, Jae-Ho Jeong, Ju Huck Lee
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	gut microbiota Odoribacter splanchnicus colorectal cancer cell-free supernatant apoptosis murine model Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/a68b317525c24e1ab6618b04593705a3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:a68b317525c24e1ab6618b04593705a3
record_format	dspace
spelling	oai:doaj.org-article:a68b317525c24e1ab6618b04593705a32021-11-17T11:03:22ZCell-Free Supernatant of Odoribacter splanchnicus Isolated From Human Feces Exhibits Anti-colorectal Cancer Activity1664-302X10.3389/fmicb.2021.736343https://doaj.org/article/a68b317525c24e1ab6618b04593705a32021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.736343/fullhttps://doaj.org/toc/1664-302XThe gut microbiota (GM) has been shown to be closely associated with the development of colorectal cancer (CRC). However, the involvement of GM is CRC has mainly been demonstrated by metagenomic profiling studies showing the compositional difference between the GM of healthy individuals and that of CRC patients and not by directly studying isolated gut microbes. Thus, to discover novel gut microbes involved in CRC, we isolated the GM from the feces of healthy individuals and evaluated its anti-CRC activity in vitro and in vivo. After GM isolation, cell-free supernatants (CFSs) were prepared from the isolated gut microorganisms to efficiently screen a large amount of the GM for anti-proliferative ability in vitro. Our results showed that the CFSs of 21 GM isolates had anti-proliferative activity against human colon cancer HCT 116 cells. Of these 21 GM isolates, GM07 was chosen for additional study because it had the highest anti-cancer activity against mouse colon cancer CT 26 cells in vitro and was further evaluated in a CT 26 allograft mouse model in vivo. GM07 was identified as Odoribacter splanchnicus through phylogenetic analysis based on 16S rRNA gene sequencing. Further investigation determined that the CFS of O. splanchnicus (OsCFS) induced anti-proliferative activity via apoptosis, but not cell cycle arrest. Moreover, GC/MS analysis suggested that the putative active molecule in OsCFS is malic acid. Finally, in the CRC mouse model, peri-tumoral injection of OsCFS significantly decreased CRC formation, compared to the control group. Altogether, these findings will provide valuable information for the discovery of potential probiotic candidates that inhibit CRC.Byeong Seob OhWon Jung ChoiJi-Sun KimSeoung Woo RyuSeung Yeob YuJung-Sook LeeSeung-Hwan ParkSe Won KangJiyoung LeeWon Yong JungYoung-Min KimJae-Ho JeongJu Huck LeeFrontiers Media S.A.articlegut microbiotaOdoribacter splanchnicuscolorectal cancercell-free supernatantapoptosismurine modelMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	gut microbiota Odoribacter splanchnicus colorectal cancer cell-free supernatant apoptosis murine model Microbiology QR1-502
spellingShingle	gut microbiota Odoribacter splanchnicus colorectal cancer cell-free supernatant apoptosis murine model Microbiology QR1-502 Byeong Seob Oh Won Jung Choi Ji-Sun Kim Seoung Woo Ryu Seung Yeob Yu Jung-Sook Lee Seung-Hwan Park Se Won Kang Jiyoung Lee Won Yong Jung Young-Min Kim Jae-Ho Jeong Ju Huck Lee Cell-Free Supernatant of Odoribacter splanchnicus Isolated From Human Feces Exhibits Anti-colorectal Cancer Activity
description	The gut microbiota (GM) has been shown to be closely associated with the development of colorectal cancer (CRC). However, the involvement of GM is CRC has mainly been demonstrated by metagenomic profiling studies showing the compositional difference between the GM of healthy individuals and that of CRC patients and not by directly studying isolated gut microbes. Thus, to discover novel gut microbes involved in CRC, we isolated the GM from the feces of healthy individuals and evaluated its anti-CRC activity in vitro and in vivo. After GM isolation, cell-free supernatants (CFSs) were prepared from the isolated gut microorganisms to efficiently screen a large amount of the GM for anti-proliferative ability in vitro. Our results showed that the CFSs of 21 GM isolates had anti-proliferative activity against human colon cancer HCT 116 cells. Of these 21 GM isolates, GM07 was chosen for additional study because it had the highest anti-cancer activity against mouse colon cancer CT 26 cells in vitro and was further evaluated in a CT 26 allograft mouse model in vivo. GM07 was identified as Odoribacter splanchnicus through phylogenetic analysis based on 16S rRNA gene sequencing. Further investigation determined that the CFS of O. splanchnicus (OsCFS) induced anti-proliferative activity via apoptosis, but not cell cycle arrest. Moreover, GC/MS analysis suggested that the putative active molecule in OsCFS is malic acid. Finally, in the CRC mouse model, peri-tumoral injection of OsCFS significantly decreased CRC formation, compared to the control group. Altogether, these findings will provide valuable information for the discovery of potential probiotic candidates that inhibit CRC.
format	article
author	Byeong Seob Oh Won Jung Choi Ji-Sun Kim Seoung Woo Ryu Seung Yeob Yu Jung-Sook Lee Seung-Hwan Park Se Won Kang Jiyoung Lee Won Yong Jung Young-Min Kim Jae-Ho Jeong Ju Huck Lee
author_facet	Byeong Seob Oh Won Jung Choi Ji-Sun Kim Seoung Woo Ryu Seung Yeob Yu Jung-Sook Lee Seung-Hwan Park Se Won Kang Jiyoung Lee Won Yong Jung Young-Min Kim Jae-Ho Jeong Ju Huck Lee
author_sort	Byeong Seob Oh
title	Cell-Free Supernatant of Odoribacter splanchnicus Isolated From Human Feces Exhibits Anti-colorectal Cancer Activity
title_short	Cell-Free Supernatant of Odoribacter splanchnicus Isolated From Human Feces Exhibits Anti-colorectal Cancer Activity
title_full	Cell-Free Supernatant of Odoribacter splanchnicus Isolated From Human Feces Exhibits Anti-colorectal Cancer Activity
title_fullStr	Cell-Free Supernatant of Odoribacter splanchnicus Isolated From Human Feces Exhibits Anti-colorectal Cancer Activity
title_full_unstemmed	Cell-Free Supernatant of Odoribacter splanchnicus Isolated From Human Feces Exhibits Anti-colorectal Cancer Activity
title_sort	cell-free supernatant of odoribacter splanchnicus isolated from human feces exhibits anti-colorectal cancer activity
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/a68b317525c24e1ab6618b04593705a3
work_keys_str_mv	AT byeongseoboh cellfreesupernatantofodoribactersplanchnicusisolatedfromhumanfecesexhibitsanticolorectalcanceractivity AT wonjungchoi cellfreesupernatantofodoribactersplanchnicusisolatedfromhumanfecesexhibitsanticolorectalcanceractivity AT jisunkim cellfreesupernatantofodoribactersplanchnicusisolatedfromhumanfecesexhibitsanticolorectalcanceractivity AT seoungwooryu cellfreesupernatantofodoribactersplanchnicusisolatedfromhumanfecesexhibitsanticolorectalcanceractivity AT seungyeobyu cellfreesupernatantofodoribactersplanchnicusisolatedfromhumanfecesexhibitsanticolorectalcanceractivity AT jungsooklee cellfreesupernatantofodoribactersplanchnicusisolatedfromhumanfecesexhibitsanticolorectalcanceractivity AT seunghwanpark cellfreesupernatantofodoribactersplanchnicusisolatedfromhumanfecesexhibitsanticolorectalcanceractivity AT sewonkang cellfreesupernatantofodoribactersplanchnicusisolatedfromhumanfecesexhibitsanticolorectalcanceractivity AT jiyounglee cellfreesupernatantofodoribactersplanchnicusisolatedfromhumanfecesexhibitsanticolorectalcanceractivity AT wonyongjung cellfreesupernatantofodoribactersplanchnicusisolatedfromhumanfecesexhibitsanticolorectalcanceractivity AT youngminkim cellfreesupernatantofodoribactersplanchnicusisolatedfromhumanfecesexhibitsanticolorectalcanceractivity AT jaehojeong cellfreesupernatantofodoribactersplanchnicusisolatedfromhumanfecesexhibitsanticolorectalcanceractivity AT juhucklee cellfreesupernatantofodoribactersplanchnicusisolatedfromhumanfecesexhibitsanticolorectalcanceractivity
_version_	1718425628981592064

Cell-Free Supernatant of Odoribacter splanchnicus Isolated From Human Feces Exhibits Anti-colorectal Cancer Activity

Ejemplares similares