Stromal protein Ecm1 regulates ureteric bud patterning and branching.

The interactions between the nephrogenic mesenchyme and the ureteric bud during kidney development are well documented. While recent studies have shed some light on the importance of the stroma during renal development, many of the signals generated in the stroma, the genetic pathways and interactio...

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Autores principales:	Suneeta S Paroly, Fengwei Wang, Lee Spraggon, Joseph Merregaert, Ekatherina Batourina, Benjamin Tycko, Kai M Schmidt-Ott, Sean Grimmond, Melissa Little, Cathy Mendelsohn
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a68c4ca108624634a444f21b3100d0dc
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spelling	oai:doaj.org-article:a68c4ca108624634a444f21b3100d0dc2021-11-18T08:39:24ZStromal protein Ecm1 regulates ureteric bud patterning and branching.1932-620310.1371/journal.pone.0084155https://doaj.org/article/a68c4ca108624634a444f21b3100d0dc2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24391906/?tool=EBIhttps://doaj.org/toc/1932-6203The interactions between the nephrogenic mesenchyme and the ureteric bud during kidney development are well documented. While recent studies have shed some light on the importance of the stroma during renal development, many of the signals generated in the stroma, the genetic pathways and interaction networks involving the stroma are yet to be identified. Our previous studies demonstrate that retinoids are crucial for branching of the ureteric bud and for patterning of the cortical stroma. In the present study we demonstrate that autocrine retinoic acid (RA) signaling in stromal cells is critical for their survival and patterning, and show that Extracellular matrix 1, Ecm1, a gene that in humans causes irritable bowel syndrome and lipoid proteinosis, is a novel RA-regulated target in the developing kidney, which is secreted from the cortical stromal cells surrounding the cap mesenchyme and ureteric bud. Our studies suggest that Ecm1 is required in the ureteric bud for regulating the distribution of Ret which is normally restricted to the tips, as inhibition of Ecm1 results in an expanded domain of Ret expression and reduced numbers of branches. We propose a model in which retinoid signaling in the stroma activates expression of Ecm1, which in turn down-regulates Ret expression in the ureteric bud cleft, where bifurcation normally occurs and normal branching progresses.Suneeta S ParolyFengwei WangLee SpraggonJoseph MerregaertEkatherina BatourinaBenjamin TyckoKai M Schmidt-OttSean GrimmondMelissa LittleCathy MendelsohnPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e84155 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Suneeta S Paroly Fengwei Wang Lee Spraggon Joseph Merregaert Ekatherina Batourina Benjamin Tycko Kai M Schmidt-Ott Sean Grimmond Melissa Little Cathy Mendelsohn Stromal protein Ecm1 regulates ureteric bud patterning and branching.
description	The interactions between the nephrogenic mesenchyme and the ureteric bud during kidney development are well documented. While recent studies have shed some light on the importance of the stroma during renal development, many of the signals generated in the stroma, the genetic pathways and interaction networks involving the stroma are yet to be identified. Our previous studies demonstrate that retinoids are crucial for branching of the ureteric bud and for patterning of the cortical stroma. In the present study we demonstrate that autocrine retinoic acid (RA) signaling in stromal cells is critical for their survival and patterning, and show that Extracellular matrix 1, Ecm1, a gene that in humans causes irritable bowel syndrome and lipoid proteinosis, is a novel RA-regulated target in the developing kidney, which is secreted from the cortical stromal cells surrounding the cap mesenchyme and ureteric bud. Our studies suggest that Ecm1 is required in the ureteric bud for regulating the distribution of Ret which is normally restricted to the tips, as inhibition of Ecm1 results in an expanded domain of Ret expression and reduced numbers of branches. We propose a model in which retinoid signaling in the stroma activates expression of Ecm1, which in turn down-regulates Ret expression in the ureteric bud cleft, where bifurcation normally occurs and normal branching progresses.
format	article
author	Suneeta S Paroly Fengwei Wang Lee Spraggon Joseph Merregaert Ekatherina Batourina Benjamin Tycko Kai M Schmidt-Ott Sean Grimmond Melissa Little Cathy Mendelsohn
author_facet	Suneeta S Paroly Fengwei Wang Lee Spraggon Joseph Merregaert Ekatherina Batourina Benjamin Tycko Kai M Schmidt-Ott Sean Grimmond Melissa Little Cathy Mendelsohn
author_sort	Suneeta S Paroly
title	Stromal protein Ecm1 regulates ureteric bud patterning and branching.
title_short	Stromal protein Ecm1 regulates ureteric bud patterning and branching.
title_full	Stromal protein Ecm1 regulates ureteric bud patterning and branching.
title_fullStr	Stromal protein Ecm1 regulates ureteric bud patterning and branching.
title_full_unstemmed	Stromal protein Ecm1 regulates ureteric bud patterning and branching.
title_sort	stromal protein ecm1 regulates ureteric bud patterning and branching.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/a68c4ca108624634a444f21b3100d0dc
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_version_	1718421519367929856

Stromal protein Ecm1 regulates ureteric bud patterning and branching.

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