POLYLACTIC ACID NANOPARTICLES INFLUENCE ON IMMUNOGENICITY OF THE PROTEIN BOUND WITH THEM

We investigated immunogenic properties of proteins bound with nanoparticles. A process for producing spherical nanoparticles having size of 20 microns by polymerization of lactic acid and an optimal method of nanoparticle surface activation were described. Activated nanoparticles were used for coval...

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Autores principales:	D. S. Polyakov, O. I. Antimonova, R. G. Sakhabeev, N. A. Grudinina, A. E. Khodova, E. S. Sinitsyna, V. A. Korzhikov-Vlakh, T. B. Tennikova, M. M. Shavlovsky
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2017
Materias:	pla-based particles virus “traps” green fluorescent protein humoral immune response Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/a69334b063fa4624950961ebd0bde8df
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spelling	oai:doaj.org-article:a69334b063fa4624950961ebd0bde8df2021-11-22T07:09:48ZPOLYLACTIC ACID NANOPARTICLES INFLUENCE ON IMMUNOGENICITY OF THE PROTEIN BOUND WITH THEM2220-76192313-739810.15789/2220-7619-2017-2-123-129https://doaj.org/article/a69334b063fa4624950961ebd0bde8df2017-06-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/512https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398We investigated immunogenic properties of proteins bound with nanoparticles. A process for producing spherical nanoparticles having size of 20 microns by polymerization of lactic acid and an optimal method of nanoparticle surface activation were described. Activated nanoparticles were used for covalent binding of model fusion protein comprising sequences of human beta-2 microglobulin and green fluorescent protein. It is shown that the nanoparticles were able to bind 3 micrograms of the protein per 1 mg of the polymer. According to the results of confocal microscopy and electrophoresis the protein is firmly adsorbed on the surface of the granules. F1 (CBA x C57BL) mice were subjected to intraperitoneal immunization with fusion protein modified nanoparticles and equivalent mixture of unmodified nanoparticles and unbound fusion protein. Blood was taken at 2 weeks after three-time intraperitoneal immunization. Antibody level to model protein was determined in mouse sera using enzyme-linked immunosorbent assay. Each of experimental and control groups comprised 39 animals. The validity of the results was evaluated using the Mann–Whitney test. It is shown that the average antibody level in the control group was 1.8 times greater than that in the experimental group. The diffe rence was significant (p < 0.004). We discuss the significance of the results in terms of development traps capable to bind virus particles in blood and to provide immune response.D. S. PolyakovO. I. AntimonovaR. G. SakhabeevN. A. GrudininaA. E. KhodovaE. S. SinitsynaV. A. Korzhikov-VlakhT. B. TennikovaM. M. ShavlovskySankt-Peterburg : NIIÈM imeni Pasteraarticlepla-based particlesvirus “traps”green fluorescent proteinhumoral immune responseInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 7, Iss 2, Pp 123-129 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	pla-based particles virus “traps” green fluorescent protein humoral immune response Infectious and parasitic diseases RC109-216
spellingShingle	pla-based particles virus “traps” green fluorescent protein humoral immune response Infectious and parasitic diseases RC109-216 D. S. Polyakov O. I. Antimonova R. G. Sakhabeev N. A. Grudinina A. E. Khodova E. S. Sinitsyna V. A. Korzhikov-Vlakh T. B. Tennikova M. M. Shavlovsky POLYLACTIC ACID NANOPARTICLES INFLUENCE ON IMMUNOGENICITY OF THE PROTEIN BOUND WITH THEM
description	We investigated immunogenic properties of proteins bound with nanoparticles. A process for producing spherical nanoparticles having size of 20 microns by polymerization of lactic acid and an optimal method of nanoparticle surface activation were described. Activated nanoparticles were used for covalent binding of model fusion protein comprising sequences of human beta-2 microglobulin and green fluorescent protein. It is shown that the nanoparticles were able to bind 3 micrograms of the protein per 1 mg of the polymer. According to the results of confocal microscopy and electrophoresis the protein is firmly adsorbed on the surface of the granules. F1 (CBA x C57BL) mice were subjected to intraperitoneal immunization with fusion protein modified nanoparticles and equivalent mixture of unmodified nanoparticles and unbound fusion protein. Blood was taken at 2 weeks after three-time intraperitoneal immunization. Antibody level to model protein was determined in mouse sera using enzyme-linked immunosorbent assay. Each of experimental and control groups comprised 39 animals. The validity of the results was evaluated using the Mann–Whitney test. It is shown that the average antibody level in the control group was 1.8 times greater than that in the experimental group. The diffe rence was significant (p < 0.004). We discuss the significance of the results in terms of development traps capable to bind virus particles in blood and to provide immune response.
format	article
author	D. S. Polyakov O. I. Antimonova R. G. Sakhabeev N. A. Grudinina A. E. Khodova E. S. Sinitsyna V. A. Korzhikov-Vlakh T. B. Tennikova M. M. Shavlovsky
author_facet	D. S. Polyakov O. I. Antimonova R. G. Sakhabeev N. A. Grudinina A. E. Khodova E. S. Sinitsyna V. A. Korzhikov-Vlakh T. B. Tennikova M. M. Shavlovsky
author_sort	D. S. Polyakov
title	POLYLACTIC ACID NANOPARTICLES INFLUENCE ON IMMUNOGENICITY OF THE PROTEIN BOUND WITH THEM
title_short	POLYLACTIC ACID NANOPARTICLES INFLUENCE ON IMMUNOGENICITY OF THE PROTEIN BOUND WITH THEM
title_full	POLYLACTIC ACID NANOPARTICLES INFLUENCE ON IMMUNOGENICITY OF THE PROTEIN BOUND WITH THEM
title_fullStr	POLYLACTIC ACID NANOPARTICLES INFLUENCE ON IMMUNOGENICITY OF THE PROTEIN BOUND WITH THEM
title_full_unstemmed	POLYLACTIC ACID NANOPARTICLES INFLUENCE ON IMMUNOGENICITY OF THE PROTEIN BOUND WITH THEM
title_sort	polylactic acid nanoparticles influence on immunogenicity of the protein bound with them
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2017
url	https://doaj.org/article/a69334b063fa4624950961ebd0bde8df
work_keys_str_mv	AT dspolyakov polylacticacidnanoparticlesinfluenceonimmunogenicityoftheproteinboundwiththem AT oiantimonova polylacticacidnanoparticlesinfluenceonimmunogenicityoftheproteinboundwiththem AT rgsakhabeev polylacticacidnanoparticlesinfluenceonimmunogenicityoftheproteinboundwiththem AT nagrudinina polylacticacidnanoparticlesinfluenceonimmunogenicityoftheproteinboundwiththem AT aekhodova polylacticacidnanoparticlesinfluenceonimmunogenicityoftheproteinboundwiththem AT essinitsyna polylacticacidnanoparticlesinfluenceonimmunogenicityoftheproteinboundwiththem AT vakorzhikovvlakh polylacticacidnanoparticlesinfluenceonimmunogenicityoftheproteinboundwiththem AT tbtennikova polylacticacidnanoparticlesinfluenceonimmunogenicityoftheproteinboundwiththem AT mmshavlovsky polylacticacidnanoparticlesinfluenceonimmunogenicityoftheproteinboundwiththem
_version_	1718418060073762816

POLYLACTIC ACID NANOPARTICLES INFLUENCE ON IMMUNOGENICITY OF THE PROTEIN BOUND WITH THEM

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