Baculovirus infection triggers a shift from amino acid starvation-induced autophagy to apoptosis.

Autophagy plays a central role in regulating important cellular functions such as cell survival during starvation and control of infectious pathogens. On the other hand, many pathogens have evolved mechanisms of inhibition of autophagy such as blockage of the formation of autophagosomes or the fusio...

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Autores principales:	Wei Wei, Zhongchao Gai, Hui Ai, Wenxian Wu, Yongbo Yang, Jianxin Peng, Huazhu Hong, Yi Li, Kaiyu Liu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a698227a967d46afb4541a70ac209eb1
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spelling	oai:doaj.org-article:a698227a967d46afb4541a70ac209eb12021-11-18T07:17:56ZBaculovirus infection triggers a shift from amino acid starvation-induced autophagy to apoptosis.1932-620310.1371/journal.pone.0037457https://doaj.org/article/a698227a967d46afb4541a70ac209eb12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22629397/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Autophagy plays a central role in regulating important cellular functions such as cell survival during starvation and control of infectious pathogens. On the other hand, many pathogens have evolved mechanisms of inhibition of autophagy such as blockage of the formation of autophagosomes or the fusion of autophagosomes with lysosomes. Baculoviruses are important insect pathogens for pest control, and autophagy activity increases significantly during insect metamorphosis. However, it is not clear whether baculovirus infection has effects on the increased autophagy. In the present study, we investigated the effects of the Autographa californica nucleopolyhedrovirus (AcMNPV) infection on autophagy in SL-HP cell line from Spodoptera litura induced under amino acid deprivation. The results revealed that AcMNPV infection did not inhibit autophagy but triggered apoptosis under starvation pressure. In the early stage of infection under starvation, mitochondrial dysfunction was detected, suggesting the organelles might be involved in cell apoptosis. The semi-quantitative PCR assay revealed that the expression of both p35 and ie-1 genes of AcMNPV had no significant difference between the starved and unstarved SL-HP cells. The western blot analysis showed that no cleavage of endogenous Atg6 occurred during the process of apoptosis in SL-HP cells. These data demonstrated that some permissive insect cells may defend baculovirus infection via apoptosis under starvation and apoptosis is independent of the cleavage of Atg6 in SL-HP cells.Wei WeiZhongchao GaiHui AiWenxian WuYongbo YangJianxin PengHuazhu HongYi LiKaiyu LiuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37457 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Wei Wei Zhongchao Gai Hui Ai Wenxian Wu Yongbo Yang Jianxin Peng Huazhu Hong Yi Li Kaiyu Liu Baculovirus infection triggers a shift from amino acid starvation-induced autophagy to apoptosis.
description	Autophagy plays a central role in regulating important cellular functions such as cell survival during starvation and control of infectious pathogens. On the other hand, many pathogens have evolved mechanisms of inhibition of autophagy such as blockage of the formation of autophagosomes or the fusion of autophagosomes with lysosomes. Baculoviruses are important insect pathogens for pest control, and autophagy activity increases significantly during insect metamorphosis. However, it is not clear whether baculovirus infection has effects on the increased autophagy. In the present study, we investigated the effects of the Autographa californica nucleopolyhedrovirus (AcMNPV) infection on autophagy in SL-HP cell line from Spodoptera litura induced under amino acid deprivation. The results revealed that AcMNPV infection did not inhibit autophagy but triggered apoptosis under starvation pressure. In the early stage of infection under starvation, mitochondrial dysfunction was detected, suggesting the organelles might be involved in cell apoptosis. The semi-quantitative PCR assay revealed that the expression of both p35 and ie-1 genes of AcMNPV had no significant difference between the starved and unstarved SL-HP cells. The western blot analysis showed that no cleavage of endogenous Atg6 occurred during the process of apoptosis in SL-HP cells. These data demonstrated that some permissive insect cells may defend baculovirus infection via apoptosis under starvation and apoptosis is independent of the cleavage of Atg6 in SL-HP cells.
format	article
author	Wei Wei Zhongchao Gai Hui Ai Wenxian Wu Yongbo Yang Jianxin Peng Huazhu Hong Yi Li Kaiyu Liu
author_facet	Wei Wei Zhongchao Gai Hui Ai Wenxian Wu Yongbo Yang Jianxin Peng Huazhu Hong Yi Li Kaiyu Liu
author_sort	Wei Wei
title	Baculovirus infection triggers a shift from amino acid starvation-induced autophagy to apoptosis.
title_short	Baculovirus infection triggers a shift from amino acid starvation-induced autophagy to apoptosis.
title_full	Baculovirus infection triggers a shift from amino acid starvation-induced autophagy to apoptosis.
title_fullStr	Baculovirus infection triggers a shift from amino acid starvation-induced autophagy to apoptosis.
title_full_unstemmed	Baculovirus infection triggers a shift from amino acid starvation-induced autophagy to apoptosis.
title_sort	baculovirus infection triggers a shift from amino acid starvation-induced autophagy to apoptosis.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/a698227a967d46afb4541a70ac209eb1
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Baculovirus infection triggers a shift from amino acid starvation-induced autophagy to apoptosis.

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