Apoptotic Activity of New Oxisterigmatocystin Derivatives from the Marine-Derived Fungus <i>Aspergillus nomius</i> NC06
Sponge-derived fungi have recently attracted attention as an important source of interesting bioactive compounds. <i>Aspergillus nomius</i> NC06 was isolated from the marine sponge <i>Neopetrosia chaliniformis</i>. This fungus was cultured on rice medium and yielded four comp...
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| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/a69c28647e00496d8f753d911f5ef12c |
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| Sumario: | Sponge-derived fungi have recently attracted attention as an important source of interesting bioactive compounds. <i>Aspergillus nomius</i> NC06 was isolated from the marine sponge <i>Neopetrosia chaliniformis</i>. This fungus was cultured on rice medium and yielded four compounds including three new oxisterigmatocystins, namely, J, K, and L (<b>1</b>, <b>2</b>, and <b>3</b>), and one known compound, aspergillicin A (<b>4</b>). Structures of the compounds were elucidated by 1D and 2D NMR spectroscopy and by high-resolution mass spectrometry. The isolated compounds were tested for cytotoxic activity against HT 29 colon cancer cells, where compounds <b>1</b>, <b>2</b>, and <b>4</b> exhibited IC<sub>50</sub> values of 6.28, 15.14, and 1.63 µM, respectively. Under the fluorescence microscope by using a double staining method, HT 29 cells were observed to be viable, apoptotic, and necrotic after treatment with the cytotoxic compounds <b>1</b>, <b>2</b>, and <b>4</b>. The result shows that compounds <b>1</b> and <b>2</b> were able to induce apoptosis and cell death in HT 29 cells. |
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