Apoptotic Activity of New Oxisterigmatocystin Derivatives from the Marine-Derived Fungus Aspergillus nomius NC06

Sponge-derived fungi have recently attracted attention as an important source of interesting bioactive compounds. Aspergillus nomius NC06 was isolated from the marine sponge Neopetrosia chaliniformis. This fungus was cultured on rice medium and yielded four comp...

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Autores principales:	Muh. Ade Artasasta, Yanwirasti Yanwirasti, Muhammad Taher, Akmal Djamaan, Ni Putu Ariantari, Ru Angelie Edrada-Ebel, Dian Handayani
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	marine sponge-derived fungus <i>Neopetrosia chaliniformis</i> <i>Aspergillus nomius</i> oxisterigmatocystin cytotoxic activity HT 29 colon cancer cells Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/a69c28647e00496d8f753d911f5ef12c
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spelling	oai:doaj.org-article:a69c28647e00496d8f753d911f5ef12c2021-11-25T18:12:56ZApoptotic Activity of New Oxisterigmatocystin Derivatives from the Marine-Derived Fungus <i>Aspergillus nomius</i> NC0610.3390/md191106311660-3397https://doaj.org/article/a69c28647e00496d8f753d911f5ef12c2021-11-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/631https://doaj.org/toc/1660-3397Sponge-derived fungi have recently attracted attention as an important source of interesting bioactive compounds. <i>Aspergillus nomius</i> NC06 was isolated from the marine sponge <i>Neopetrosia chaliniformis</i>. This fungus was cultured on rice medium and yielded four compounds including three new oxisterigmatocystins, namely, J, K, and L (<b>1</b>, <b>2</b>, and <b>3</b>), and one known compound, aspergillicin A (<b>4</b>). Structures of the compounds were elucidated by 1D and 2D NMR spectroscopy and by high-resolution mass spectrometry. The isolated compounds were tested for cytotoxic activity against HT 29 colon cancer cells, where compounds <b>1</b>, <b>2</b>, and <b>4</b> exhibited IC<sub>50</sub> values of 6.28, 15.14, and 1.63 µM, respectively. Under the fluorescence microscope by using a double staining method, HT 29 cells were observed to be viable, apoptotic, and necrotic after treatment with the cytotoxic compounds <b>1</b>, <b>2</b>, and <b>4</b>. The result shows that compounds <b>1</b> and <b>2</b> were able to induce apoptosis and cell death in HT 29 cells.Muh. Ade ArtasastaYanwirasti YanwirastiMuhammad TaherAkmal DjamaanNi Putu AriantariRu Angelie Edrada-EbelDian HandayaniMDPI AGarticlemarine sponge-derived fungus<i>Neopetrosia chaliniformis</i><i>Aspergillus nomius</i>oxisterigmatocystincytotoxic activityHT 29 colon cancer cellsBiology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 631, p 631 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	marine sponge-derived fungus <i>Neopetrosia chaliniformis</i> <i>Aspergillus nomius</i> oxisterigmatocystin cytotoxic activity HT 29 colon cancer cells Biology (General) QH301-705.5
spellingShingle	marine sponge-derived fungus <i>Neopetrosia chaliniformis</i> <i>Aspergillus nomius</i> oxisterigmatocystin cytotoxic activity HT 29 colon cancer cells Biology (General) QH301-705.5 Muh. Ade Artasasta Yanwirasti Yanwirasti Muhammad Taher Akmal Djamaan Ni Putu Ariantari Ru Angelie Edrada-Ebel Dian Handayani Apoptotic Activity of New Oxisterigmatocystin Derivatives from the Marine-Derived Fungus <i>Aspergillus nomius</i> NC06
description	Sponge-derived fungi have recently attracted attention as an important source of interesting bioactive compounds. <i>Aspergillus nomius</i> NC06 was isolated from the marine sponge <i>Neopetrosia chaliniformis</i>. This fungus was cultured on rice medium and yielded four compounds including three new oxisterigmatocystins, namely, J, K, and L (<b>1</b>, <b>2</b>, and <b>3</b>), and one known compound, aspergillicin A (<b>4</b>). Structures of the compounds were elucidated by 1D and 2D NMR spectroscopy and by high-resolution mass spectrometry. The isolated compounds were tested for cytotoxic activity against HT 29 colon cancer cells, where compounds <b>1</b>, <b>2</b>, and <b>4</b> exhibited IC<sub>50</sub> values of 6.28, 15.14, and 1.63 µM, respectively. Under the fluorescence microscope by using a double staining method, HT 29 cells were observed to be viable, apoptotic, and necrotic after treatment with the cytotoxic compounds <b>1</b>, <b>2</b>, and <b>4</b>. The result shows that compounds <b>1</b> and <b>2</b> were able to induce apoptosis and cell death in HT 29 cells.
format	article
author	Muh. Ade Artasasta Yanwirasti Yanwirasti Muhammad Taher Akmal Djamaan Ni Putu Ariantari Ru Angelie Edrada-Ebel Dian Handayani
author_facet	Muh. Ade Artasasta Yanwirasti Yanwirasti Muhammad Taher Akmal Djamaan Ni Putu Ariantari Ru Angelie Edrada-Ebel Dian Handayani
author_sort	Muh. Ade Artasasta
title	Apoptotic Activity of New Oxisterigmatocystin Derivatives from the Marine-Derived Fungus <i>Aspergillus nomius</i> NC06
title_short	Apoptotic Activity of New Oxisterigmatocystin Derivatives from the Marine-Derived Fungus <i>Aspergillus nomius</i> NC06
title_full	Apoptotic Activity of New Oxisterigmatocystin Derivatives from the Marine-Derived Fungus <i>Aspergillus nomius</i> NC06
title_fullStr	Apoptotic Activity of New Oxisterigmatocystin Derivatives from the Marine-Derived Fungus <i>Aspergillus nomius</i> NC06
title_full_unstemmed	Apoptotic Activity of New Oxisterigmatocystin Derivatives from the Marine-Derived Fungus <i>Aspergillus nomius</i> NC06
title_sort	apoptotic activity of new oxisterigmatocystin derivatives from the marine-derived fungus <i>aspergillus nomius</i> nc06
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/a69c28647e00496d8f753d911f5ef12c
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Apoptotic Activity of New Oxisterigmatocystin Derivatives from the Marine-Derived Fungus <i>Aspergillus nomius</i> NC06

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