The Candida albicans Ku70 modulates telomere length and structure by regulating both telomerase and recombination.

The heterodimeric Ku complex has been shown to participate in DNA repair and telomere regulation in a variety of organisms. Here we report a detailed characterization of the function of Ku70 in the diploid fungal pathogen Candida albicans. Both ku70 heterozygous and homozygous deletion mutants have...

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Autores principales:	Lidia Chico, Toni Ciudad, Min Hsu, Neal F Lue, Germán Larriba
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/a6a023e3682d4b13a61607351dc3c509
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spelling	oai:doaj.org-article:a6a023e3682d4b13a61607351dc3c5092021-11-18T06:47:28ZThe Candida albicans Ku70 modulates telomere length and structure by regulating both telomerase and recombination.1932-620310.1371/journal.pone.0023732https://doaj.org/article/a6a023e3682d4b13a61607351dc3c5092011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21886818/?tool=EBIhttps://doaj.org/toc/1932-6203The heterodimeric Ku complex has been shown to participate in DNA repair and telomere regulation in a variety of organisms. Here we report a detailed characterization of the function of Ku70 in the diploid fungal pathogen Candida albicans. Both ku70 heterozygous and homozygous deletion mutants have a wild-type colony and cellular morphology, and are not sensitive to MMS or UV light. Interestingly, we observed complex effects of KU70 gene dosage on telomere lengths, with the KU70/ku70 heterozygotes exhibiting slightly shorter telomeres, and the ku70 null strain exhibiting long and heterogeneous telomeres. Analysis of combination mutants suggests that the telomere elongation in the ku70 null mutant is due mostly to unregulated telomerase action. In addition, elevated levels of extrachromosomal telomeric circles were detected in the null mutant, consistent with activation of aberrant telomeric recombination. Altogether, our observations point to multiple mechanisms of the Ku complex in telomerase regulation and telomere protection in C. albicans, and reveal interesting similarities and differences in the mechanisms of the Ku complex in disparate systems.Lidia ChicoToni CiudadMin HsuNeal F LueGermán LarribaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 8, p e23732 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lidia Chico Toni Ciudad Min Hsu Neal F Lue Germán Larriba The Candida albicans Ku70 modulates telomere length and structure by regulating both telomerase and recombination.
description	The heterodimeric Ku complex has been shown to participate in DNA repair and telomere regulation in a variety of organisms. Here we report a detailed characterization of the function of Ku70 in the diploid fungal pathogen Candida albicans. Both ku70 heterozygous and homozygous deletion mutants have a wild-type colony and cellular morphology, and are not sensitive to MMS or UV light. Interestingly, we observed complex effects of KU70 gene dosage on telomere lengths, with the KU70/ku70 heterozygotes exhibiting slightly shorter telomeres, and the ku70 null strain exhibiting long and heterogeneous telomeres. Analysis of combination mutants suggests that the telomere elongation in the ku70 null mutant is due mostly to unregulated telomerase action. In addition, elevated levels of extrachromosomal telomeric circles were detected in the null mutant, consistent with activation of aberrant telomeric recombination. Altogether, our observations point to multiple mechanisms of the Ku complex in telomerase regulation and telomere protection in C. albicans, and reveal interesting similarities and differences in the mechanisms of the Ku complex in disparate systems.
format	article
author	Lidia Chico Toni Ciudad Min Hsu Neal F Lue Germán Larriba
author_facet	Lidia Chico Toni Ciudad Min Hsu Neal F Lue Germán Larriba
author_sort	Lidia Chico
title	The Candida albicans Ku70 modulates telomere length and structure by regulating both telomerase and recombination.
title_short	The Candida albicans Ku70 modulates telomere length and structure by regulating both telomerase and recombination.
title_full	The Candida albicans Ku70 modulates telomere length and structure by regulating both telomerase and recombination.
title_fullStr	The Candida albicans Ku70 modulates telomere length and structure by regulating both telomerase and recombination.
title_full_unstemmed	The Candida albicans Ku70 modulates telomere length and structure by regulating both telomerase and recombination.
title_sort	candida albicans ku70 modulates telomere length and structure by regulating both telomerase and recombination.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/a6a023e3682d4b13a61607351dc3c509
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The Candida albicans Ku70 modulates telomere length and structure by regulating both telomerase and recombination.

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